Rabbit Anti-ACLY Recombinant Antibody (clone 3A5) (CAT#: ZG-0526J)

Figure 1 Rabbit Anti-ACLY Antibody (ZG-0526J) in WB

Western Blot
Positive WB detected in: L02 whole cell lysate, K562 whole cell lysate, Hela whole cell lysate, Raji whole cell lysate, A549 whole cell lysate
All lanes: ACLY antibody at 1:1500
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 121, 120, 92 kDa
Observed band size: 120 kDa

Figure 2 Rabbit Anti-ACLY Antibody (ZG-0526J) in IF

Immunofluorescence staining of HepG2 Cells with ZG-0526J at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).

Figure 3 Rabbit Anti-ACLY Antibody (ZG-0526J) in FC

Overlay histogram showing Hela cells stained with ZG-0526J (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followedby the antibody (1µg/1*106cells) for 1 h at 4°C. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4°C. Control antibody (green line) was Rabbit IgG (1µg/1*106cells) used under the same conditions. Acquisition of>10,000 events was performed.