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  • Rabbit Anti-ACLY Recombinant Antibody (VS3-CJ1034)

Rabbit Anti-ACLY Recombinant Antibody (VS3-CJ1034) (CAT#: VS3-CJ1034)

This product is a rabbit antibody that recognizes human, mouse, and rat ACLY.


See more Engineered Antibody products for ACLY
Specific Inquiry
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  • Conjugation:
  • Endotoxin:
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  • Gene Expression
  • Datasheet
  • MSDS
  • COA
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line A-431
Figure 1 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/28758/if_selected.jpg
Figure 2 IHC staining of human kidney
Figure 2 IHC staining of human kidney
(Immunohistochemical staining of human kidney shows distinct nuclear and cytoplasmic positivity in cells in tubules.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/7783/ihc_selected.jpg
Figure 3 IF staining of human cell line U-2 OS
Figure 3 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/22434/395_D3_1_blue_red_green.jpg
Figure 4 IF staining of human cell line U-251 MG
Figure 4 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/22434/399_D3_1_blue_red_green.jpg
Normal Tissue
Figure 5 Colon
Figure 5 Colon
(Peripheral nerve/ganglion
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/22959/49962_A_9_3.jpg
Figure 6 Pancreas
Figure 6 Pancreas
(Pancreatic endocrine cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/22959/49962_A_3_3.jpg
Figure 7 Kidney
Figure 7 Kidney
(Cells in tubules
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/22959/49962_A_7_5.jpg
Figure 8 Testis
Figure 8 Testis
(Cells in seminiferous ducts
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous nuclear
Leydig cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/22959/49962_A_6_6.jpg
RNA Expression
Figure 9 RNA cell line category: Low cell line specificity
Figure 9 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000131473-ACLY

Specifications

  • Immunogen
  • Recombinant protein
  • Host Species
  • Rabbit
  • Type
  • Rabbit IgG
  • Specificity
  • Human, Mouse, Rat ACLY
  • Species Reactivity
  • Human, Mouse, Rat
  • Applications
  • WB, ICC, IF, IHC, IP, FC
  • Conjugate
  • Unconjugated

Product Property

  • Purification
  • Protein A affinity purified
  • Purity
  • >95% as determined by SDS-PAGE
  • Format
  • Liquid
  • Buffer
  • 40% Glycerol, 1% BSA, TBS, pH7.4.
  • Preservative
  • 0.05% Sodium Azide
  • Storage
  • Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • This antibody has been tested for use in Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Flow Cytometry.

Target

  • Alternative Names
  • ACL; ATPCL; CLATP
  • Sequence Similarities
  • In the N-terminal section: belongs to the succinate/malate CoA ligase beta subunit family.
    In the C-terminal section: belongs to the succinate/malate CoA ligase alpha subunit family.
  • Cellular Localization
  • Cytoplasm
  • Post Translation Modifications
  • Phosphorylated by PKA and GSK3 in a sequential manner; phosphorylation results in activation of its activity (PubMed:10653665).
    Phosphorylation on Thr-447 and Ser-451 depends on the phosphorylation state of Ser-455 (By similarity).
    Phosphorylation on Ser-455 is decreased by prior phosphorylation on the other 2 residues (By similarity).
    ISGylated.
    Acetylated at Lys-540, Lys-546 and Lys-554 by KAT2B/PCAF (PubMed:23932781).
    Acetylation is promoted by glucose and stabilizes the protein, probably by preventing ubiquitination at the same sites (PubMed:23932781).
    Acetylation promotes de novo lipid synthesis (PubMed:23932781).
    Deacetylated by SIRT2.
    Ubiquitinated at Lys-540, Lys-546 and Lys-554 by the BCR(KLHL25) E3 ubiquitin ligase complex and UBR4, leading to its degradation (PubMed:23932781, PubMed:27664236, PubMed:34491895).
    Ubiquitination is probably inhibited by acetylation at same site (PubMed:23932781).
    BCR(KLHL25)-mediated degradation of ACLY promotes fatty acid oxidation and is required for differentiation of inducible regulatory T (iTreg) cells (PubMed:34491895).
  • Function
  • Catalyzes the cleavage of citrate into oxaloacetate and acetyl-CoA, the latter serving as common substrate for de novo cholesterol and fatty acid synthesis.

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Isotype Control

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "ACLY"

Single-domain Antibody

CAT Product Name Application Type
NAB-17-VHH Recombinant Anti-human ACLY VHH Single Domain Antibody IHC, IP, FC, Neut, FUNC Llama VHH

Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-0038 Hi-Affi™ Rabbit Anti-ACLY Recombinant Antibody (clone DS38AB) WB, FC, IP Rabbit IgG

Recombinant Antibody

CAT Product Name Application Type
MRO-0140-CN Rabbit Anti-ACLY Recombinant Antibody (clone CBACN-054) WB, IF, IHC, IP, FC Rabbit IgG
MRO-1666-CN Rabbit Anti-ACLY Polyclonal Antibody (MRO-1666-CN) WB, IF, IHC, FC Rabbit IgG
ZG-0526J Rabbit Anti-ACLY Recombinant Antibody (clone 3A5) ELISA, WB, IF, FC Rabbit IgG
VS3-WK681 Rabbit Anti-ACLY Recombinant Antibody (clone JU80-30) WB, ELISA Rabbit IgG
VS4-WK9 Mouse Anti-ACLY Recombinant Antibody (VS4-WK9) ELISA, WB, ICC, FC Mouse IgG2a

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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