Rabbit Anti-ACLY Recombinant Antibody (VS3-CJ1034)
CAT#: VS3-CJ1034
This product is a rabbit antibody that recognizes human, mouse, and rat ACLY.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/28758/if_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human kidney
Immunohistochemical staining of human kidney shows distinct nuclear and cytoplasmic positivity in cells in tubules.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/7783/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 3 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/22434/395_D3_1_blue_red_green.jpg
Subcellular Location and Protein Expression
Figure 4 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/22434/399_D3_1_blue_red_green.jpg
Normal Tissue
Figure 5 Colon
Peripheral nerve/ganglion
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/22959/49962_A_9_3.jpg
Normal Tissue
Figure 6 Pancreas
Pancreatic endocrine cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/22959/49962_A_3_3.jpg
Normal Tissue
Figure 7 Kidney
Cells in tubules
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/22959/49962_A_7_5.jpg
Normal Tissue
Figure 8 Testis
Cells in seminiferous ducts
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous nuclear
Leydig cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/22959/49962_A_6_6.jpg
RNA Expression
Figure 9 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000131473-ACLY
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Specifications
- Immunogen
- Recombinant protein
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse, Rat ACLY
- Species Reactivity
- Human, Mouse, Rat
- Applications
- WB, ICC, IF, IHC, IP, FC
- Conjugate
- Unconjugated
Product Property
- Purification
- Protein A affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Buffer
- 40% Glycerol, 1% BSA, TBS, pH7.4.
- Preservative
- 0.05% Sodium Azide
- Storage
- Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Flow Cytometry.
Target
- Alternative Names
- ACL; ATPCL; CLATP
- Gene ID
- 47
- UniProt ID
- P53396
- Sequence Similarities
- In the N-terminal section: belongs to the succinate/malate CoA ligase beta subunit family.
In the C-terminal section: belongs to the succinate/malate CoA ligase alpha subunit family.
- Cellular Localization
- Cytoplasm
- Post Translation Modifications
- Phosphorylated by PKA and GSK3 in a sequential manner; phosphorylation results in activation of its activity (PubMed:10653665).
Phosphorylation on Thr-447 and Ser-451 depends on the phosphorylation state of Ser-455 (By similarity).
Phosphorylation on Ser-455 is decreased by prior phosphorylation on the other 2 residues (By similarity).
ISGylated.
Acetylated at Lys-540, Lys-546 and Lys-554 by KAT2B/PCAF (PubMed:23932781).
Acetylation is promoted by glucose and stabilizes the protein, probably by preventing ubiquitination at the same sites (PubMed:23932781).
Acetylation promotes de novo lipid synthesis (PubMed:23932781).
Deacetylated by SIRT2.
Ubiquitinated at Lys-540, Lys-546 and Lys-554 by the BCR(KLHL25) E3 ubiquitin ligase complex and UBR4, leading to its degradation (PubMed:23932781, PubMed:27664236, PubMed:34491895).
Ubiquitination is probably inhibited by acetylation at same site (PubMed:23932781).
BCR(KLHL25)-mediated degradation of ACLY promotes fatty acid oxidation and is required for differentiation of inducible regulatory T (iTreg) cells (PubMed:34491895).
- Protein Refseq
- NP_001087.2; NP_001290203.1; NP_001290204.1
- Function
- Catalyzes the cleavage of citrate into oxaloacetate and acetyl-CoA, the latter serving as common substrate for de novo cholesterol and fatty acid synthesis.
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "ACLY"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NAB-17-VHH | Recombinant Anti-human ACLY VHH Single Domain Antibody | IHC, IP, FC, Neut, FUNC | Llama VHH |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0038 | Hi-Affi™ Rabbit Anti-ACLY Recombinant Antibody (clone DS38AB) | WB, FC, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-0140-CN | Rabbit Anti-ACLY Recombinant Antibody (clone CBACN-054) | WB, IF, IHC, IP, FC | Rabbit IgG |
| MRO-1666-CN | Rabbit Anti-ACLY Polyclonal Antibody (MRO-1666-CN) | WB, IF, IHC, FC | Rabbit IgG |
| VS3-QX6 | Mouse Anti-ACLY Recombinant Antibody (clone 5F8D11) | WB, IHC, ICC, FC, ELISA | Mouse IgG1 |
| VS3-FY16 | Recombinant Rabbit Anti-ACLY Antibody (clone R02-9B5) | WB, ICC, IF, IP | Rabbit IgG |
| VS7-HM16 | Mouse Anti-ACLY Recombinant Antibody (clone CBL010HM) | WB, IF, ICC, FCM | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC691 | AbPlus™ Anti-ACLY Magnetic Beads (VS-0724-YC691) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY21 | Anti-ACLY Immunohistochemistry Kit | IHC | |
| VS-0525-XY78 | Anti-Mouse ACLY Immunohistochemistry Kit | IHC | |
| VS-0525-XY77 | Anti-Human ACLY Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC17 | CytoStream™ Mouse Anti-ACLY Recombinant Antibody (VS13-YC17) | WB, IF, FC | Mouse IgG2a |
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