Rabbit Anti-ACLY Recombinant Antibody (VS3-CJ1034) (CAT#: VS3-CJ1034)

• Immunogen
• Recombinant protein

• Host Species
• Rabbit

• Type
• Rabbit IgG

• Specificity
• Human, Mouse, Rat ACLY

• Species Reactivity
• Human, Mouse, Rat

• Applications
• WB, ICC, IF, IHC, IP, FC

• Conjugate
• Unconjugated

• Purification
• Protein A affinity purified

• Purity
• >95% as determined by SDS-PAGE

• Format
• Liquid

• Buffer
• 40% Glycerol, 1% BSA, TBS, pH7.4.

• Preservative
• 0.05% Sodium Azide

• Storage
• Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.

• Application Notes
• This antibody has been tested for use in Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Flow Cytometry.

• Alternative Names
• ACL; ATPCL; CLATP

• Gene ID
• 47

• UniProt ID
• P53396

• Sequence Similarities
• In the N-terminal section: belongs to the succinate/malate CoA ligase beta subunit family.
  In the C-terminal section: belongs to the succinate/malate CoA ligase alpha subunit family.

• Cellular Localization
• Cytoplasm

• Post Translation Modifications
• Phosphorylated by PKA and GSK3 in a sequential manner; phosphorylation results in activation of its activity (PubMed:10653665).
  Phosphorylation on Thr-447 and Ser-451 depends on the phosphorylation state of Ser-455 (By similarity).
  Phosphorylation on Ser-455 is decreased by prior phosphorylation on the other 2 residues (By similarity).
  ISGylated.
  Acetylated at Lys-540, Lys-546 and Lys-554 by KAT2B/PCAF (PubMed:23932781).
  Acetylation is promoted by glucose and stabilizes the protein, probably by preventing ubiquitination at the same sites (PubMed:23932781).
  Acetylation promotes de novo lipid synthesis (PubMed:23932781).
  Deacetylated by SIRT2.
  Ubiquitinated at Lys-540, Lys-546 and Lys-554 by the BCR(KLHL25) E3 ubiquitin ligase complex and UBR4, leading to its degradation (PubMed:23932781, PubMed:27664236, PubMed:34491895).
  Ubiquitination is probably inhibited by acetylation at same site (PubMed:23932781).
  BCR(KLHL25)-mediated degradation of ACLY promotes fatty acid oxidation and is required for differentiation of inducible regulatory T (iTreg) cells (PubMed:34491895).

• Protein Refseq
• NP_001087.2; NP_001290203.1; NP_001290204.1

• Function
• Catalyzes the cleavage of citrate into oxaloacetate and acetyl-CoA, the latter serving as common substrate for de novo cholesterol and fatty acid synthesis.

Isotype Control

• MOB-208CQ
• Rabbit IgG Isotype Control Antibody

Secondary Antibody

• 2ND-057CQ
• Donkey Anti-Rabbit IgG (H&L) Antibody

• 2ND-059CQ
• Goat Anti-Rabbit IgG (H&L) Antibody

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