Mouse Anti-ACLY Recombinant Antibody (VS4-WK9)
CAT#: VS4-WK9
This product is a mouse monoclonal antibody that recognizes ACLY and can be used in ELISA, WB, ICC, FC.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/28758/if_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human kidney
Immunohistochemical staining of human kidney shows distinct nuclear and cytoplasmic positivity in cells in tubules.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/7783/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 3 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/22434/395_D3_1_blue_red_green.jpg
Subcellular Location and Protein Expression
Figure 4 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/22434/399_D3_1_blue_red_green.jpg
Normal Tissue
Figure 5 Colon
Peripheral nerve/ganglion
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/22959/49962_A_9_3.jpg
Normal Tissue
Figure 6 Pancreas
Pancreatic endocrine cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/22959/49962_A_3_3.jpg
Normal Tissue
Figure 7 Kidney
Cells in tubules
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/22959/49962_A_7_5.jpg
Normal Tissue
Figure 8 Testis
Cells in seminiferous ducts
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous nuclear
Leydig cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/22959/49962_A_6_6.jpg
RNA Expression
Figure 9 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000131473-ACLY
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Specifications
- Immunogen
- Recombinant fragment, corresponding to amino acids 734aa-983aa of human ACLY (C-terminus).
- Host Species
- Mouse
- Derivation
- Mouse
- Type
- Mouse IgG2a
- Specificity
- Human ACLY
- Species Reactivity
- Human, Mouse, Rat, Pig
- Applications
- ELISA, WB, ICC, FC
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 0.1M Tris-Glycine (pH 7.4), 150 mM NaCl, and 50% glycerol.
- Preservative
- 0.2% sodium azide
- Storage
- Store at -20°C or -80°C. Avoid repeated freeze.
- Shipping
- Ice packs
Applications
- Application Notes
- Recommended dilution: WB:1:500-1:5000, ICC:1:50-1:200
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "ACLY"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NAB-17-VHH | Recombinant Anti-human ACLY VHH Single Domain Antibody | IHC, IP, FC, Neut, FUNC | Llama VHH |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0038 | Hi-Affi™ Rabbit Anti-ACLY Recombinant Antibody (clone DS38AB) | WB, FC, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-0140-CN | Rabbit Anti-ACLY Recombinant Antibody (clone CBACN-054) | WB, IF, IHC, IP, FC | Rabbit IgG |
| VS3-WK681 | Rabbit Anti-ACLY Recombinant Antibody (clone JU80-30) | WB, ELISA | Rabbit IgG |
| VS3-CJ98 | Mouse Anti-ACLY Recombinant Antibody (VS3-CJ98) | WB, ELISA | Mouse IgG |
| VS3-FY16 | Recombinant Rabbit Anti-ACLY Antibody (clone R02-9B5) | WB, ICC, IF, IP | Rabbit IgG |
| VS7-HM16 | Mouse Anti-ACLY Recombinant Antibody (clone CBL010HM) | WB, IF, ICC, FCM | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC691 | AbPlus™ Anti-ACLY Magnetic Beads (VS-0724-YC691) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY21 | Anti-ACLY Immunohistochemistry Kit | IHC | |
| VS-0525-XY78 | Anti-Mouse ACLY Immunohistochemistry Kit | IHC | |
| VS-0525-XY77 | Anti-Human ACLY Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC17 | CytoStream™ Mouse Anti-ACLY Recombinant Antibody (VS13-YC17) | WB, IF, FC | Mouse IgG2a |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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