Rabbit Anti-ACO2 Recombinant Antibody (clone R07-1F4)
CAT#: VS3-FY1963
This rabbit recombinant monoclonal antibody has been developed to specifically bind to human ACO2. It has been shown to cross-react with mouse and rat. This antibody is highly versatile and can be effectively utilized in a range of assays, such as western blot, immunohistochemistry-frozen, immunohistochemistry-paraffin, as well as immunocytochemistry/immunofluorescence. The recommended dilutions for use are as follows: western blot (dilution 1/500-1/1000), immunohistochemistry (dilution 1/50-1/100), immunofluorescence (dilution 1/50-1/200).
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1097/if_selected.jpg
Normal Tissue
Figure 2 High expression in heart muscle
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1097/2509_B_6_6_val_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Glial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1097/2509_B_9_5.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1097/2509_A_7_3.jpg
Normal Tissue
Figure 5 Liver
Cholangiocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1097/2509_A_7_4.jpg
Normal Tissue
Figure 6 Kidney
Bowman's capsule Staining: High Intensity: Strong Quantity:>75% Cells in glomeruli Staining: High Intensity: Strong Quantity:>75% Collecting ducts Staining: High Intensity: Strong Quantity:>75% Distal tubules Staining: High Intensity: Strong Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1097/2509_A_7_5.jpg
Normal Tissue
Figure 7 Testis
Leydig cells Staining: High Intensity: Strong Quantity:>75% Pachytene spermatocytes Staining: High Intensity: Strong Quantity: 75%-25% Sertoli cells Staining: High Intensity: Strong Quantity:>75% Spermatogonia cells Staining: High Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1097/2509_A_5_6.jpg
Normal Tissue
Figure 8 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1097/2509_A_8_8.jpg
RNA Expression
Figure 9 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000100412-ACO2
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ICC
Figure 1 Anti-ACO2 Antibody (VS3-FY1963) in ICC.
Immunocytochemistry staining of Aconitase 2 (green) in CEM using the anti-Aconitase 2 antibody. Nuclear DNA was labeled with DAPI (shown in blue).
WB
Figure 2 Anti-ACO2 Antibody (VS3-FY1963) in WB.
The Aconitase 2 expression in 3T3, mouse liver lysates were detected using the anti-Aconitase 2 by western blot assay.
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Specifications
- Immunogen
- Synthetic peptide of human aconitase 2
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat ACO2
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R07-1F4
- Applications
- Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunofluorescence
- MW
- The calculated weight of the target protein is approximately 85 kDa, while the observed molecular weight stands at 85 kDa.
- Related Disease
- Infantile Cerebellar-Retinal Degeneration and Optic Atrophy 9. Among its related pathways are TCA cycle III (animals) and Peroxisomal lipid metabolism
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Immunofluorescence: 1/50-1/200
Target
- Alternative Names
- Aconitase 2; Aconitate Hydratase, Mitochondrial; Aconitase 2, Mitochondrial; Mitochondrial Aconitase; Citrate Hydro-Lyase; EC 4.2.1.3; Epididymis Secretory Sperm Binding Protein Li 284; Aconitase
- Gene ID
- 50
- UniProt ID
- Q99798
- Sequence Similarities
- Belongs to the aconitase/IPM isomerase family.
- Cellular Localization
- Mitochondrion
- Post Translation Modifications
- Forms covalent cross-links mediated by transglutaminase TGM2, between a glutamine and the epsilon-amino group of a lysine residue, forming homopolymers and heteropolymers. (Q99798-ACON_HUMAN)
Modification sites at PhosphoSitePlus (Q99798)
Modification sites at neXtProt (NX_Q99798)
Glycosylation from GlyGen (Q99798) 1 site, 1 O-linked glycan (1 site)
- Protein Refseq
- XP_054181635.1; NP_001089.1
- Function
- Catalyzes the isomerization of citrate to isocitrate via cis-aconitate.
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "ACO2"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0040 | Hi-Affi™ Rabbit Anti-ACO2 Recombinant Antibody (clone DS40AB) | WB, IHC, ICC, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-0023-CN | Rabbit Anti-ACO2 Recombinant Antibody (clone JE40-83) | WB, IF, IHC, IP | Rabbit IgG |
| ZG-0066U | Rabbit Anti-ACO2 Recombinant Antibody (clone 8H4) | ELISA, IHC | Rabbit IgG |
| VS3-WK1122 | Mouse Anti-ACO2 Recombinant Antibody (VS3-WK1122) | WB, ELISA | Mouse IgG1 |
| VS3-FY17 | Recombinant Rabbit Anti-ACO2 Antibody (clone R07-7A1) | WB, IHC-F, IHC-P, ICC, IF, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC608 | AbPlus™ Anti-ACO2 Magnetic Beads (VS-0724-YC608) | IP, Protein Purification | |
| VS-0724-YC609 | AbPlus™ Anti-Aco2 Magnetic Beads (VS-0724-YC609) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY5 | Mouse Anti-NHP ACO2 Recombinant Antibody (clone 7G4) | WB, IHC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY22 | Anti-ACO2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY80 | Anti-Mouse ACO2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY79 | Anti-Human ACO2 Immunohistochemistry Kit | IHC |
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