Rabbit Anti-ACO2 Recombinant Antibody (clone R07-1F4) (CAT#: VS3-FY1963)
This rabbit recombinant monoclonal antibody has been developed to specifically bind to human ACO2. It has been shown to cross-react with mouse and rat. This antibody is highly versatile and can be effectively utilized in a range of assays, such as western blot, immunohistochemistry-frozen, immunohistochemistry-paraffin, as well as immunocytochemistry/immunofluorescence. The recommended dilutions for use are as follows: western blot (dilution 1/500-1/1000), immunohistochemistry (dilution 1/50-1/100), immunofluorescence (dilution 1/50-1/200).
Specific Inquiry
ICC
Figure 1 Anti-ACO2 Antibody (VS3-FY1963) in ICC.
Immunocytochemistry staining of Aconitase 2 (green) in CEM using the anti-Aconitase 2 antibody. Nuclear DNA was labeled with DAPI (shown in blue).
WB
Figure 2 Anti-ACO2 Antibody (VS3-FY1963) in WB.
The Aconitase 2 expression in 3T3, mouse liver lysates were detected using the anti-Aconitase 2 by western blot assay.
Specifications
- Immunogen
- Synthetic peptide of human aconitase 2
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat ACO2
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R07-1F4
- Applications
- Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunofluorescence
- MW
- The calculated weight of the target protein is approximately 85 kDa, while the observed molecular weight stands at 85 kDa.
- Related Disease
- Infantile Cerebellar-Retinal Degeneration and Optic Atrophy 9. Among its related pathways are TCA cycle III (animals) and Peroxisomal lipid metabolism
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Immunofluorescence: 1/50-1/200
Target
- Alternative Names
- Aconitase 2; Aconitate Hydratase, Mitochondrial; Aconitase 2, Mitochondrial; Mitochondrial Aconitase; Citrate Hydro-Lyase; EC 4.2.1.3; Epididymis Secretory Sperm Binding Protein Li 284; Aconitase
- Gene ID
- 50
- UniProt ID
- Q99798
- Sequence Similarities
- Belongs to the aconitase/IPM isomerase family.
- Cellular Localization
- Mitochondrion
- Post Translation Modifications
- Forms covalent cross-links mediated by transglutaminase TGM2, between a glutamine and the epsilon-amino group of a lysine residue, forming homopolymers and heteropolymers. (Q99798-ACON_HUMAN)
Modification sites at PhosphoSitePlus (Q99798)
Modification sites at neXtProt (NX_Q99798)
Glycosylation from GlyGen (Q99798) 1 site, 1 O-linked glycan (1 site)
- Protein Refseq
- XP_054181635.1; NP_001089.1
- Function
- Catalyzes the isomerization of citrate to isocitrate via cis-aconitate.
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Rabbit Monoclonal Antibody Products
IgG Antibody Products
Antibody Magnetic Beads
Non-Human Primate (NHP) Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0040 | Hi-Affi™ Rabbit Anti-ACO2 Recombinant Antibody (clone DS40AB) | WB, IHC, ICC, IP | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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