Mouse Anti-ACP3 Recombinant Antibody (clone PT2122) (CAT#: VS3-CJ670)

• Immunogen
• Synthesized peptide derived from human Prostatic Acid Phosphatase (PSAP)

• Host Species
• Mouse

• Type
• Mouse IgG1, κ

• Specificity
• Human ACP3

• Species Reactivity
• Human

• Clone
• PT2122

• Applications
• IHC-P

• Conjugate
• Unconjugated

• Purification
• Affinity Chromatography

• Purity
• >95% as determined by SDS-PAGE

• Format
• Liquid

• Buffer
• 50% Glycerol, 0.5% BSA, PBS.

• Preservative
• 0.02% Sodium Azide

• Storage
• Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.

• Application Notes
• This antibody has been tested for use in Immunohistochemistry-Parraffin (1:100-1:500).

• Alternative Names
• ACPP; 5'-NT; ACP-3; TM-PAP

• Gene ID
• 55

• UniProt ID
• P15309

• Sequence Similarities
• Belongs to the histidine acid phosphatase family.

• Cellular Localization
• Amyloid, Cell membrane, Cytoplasm, Lysosome, Membrane, Nucleus, Secreted

• Post Translation Modifications
• N-glycosylated. High mannose content, partially sialylated and fucosylated biantennary complex. Also fucosylated with partially sialylated triantennary complex oligosaccharides.
  Proteolytically cleaved in seminal fluid to produce several peptides. Peptide PAPf39, the most prominent, forms amyloid beta-sheet fibrils, SEVI (semen-derived enhancer of viral infection).

• Protein Refseq
• NP_001090.2; NP_001127666.1; NP_001278966.1

• Function
• A non-specific tyrosine phosphatase that dephosphorylates a diverse number of substrates under acidic conditions (pH 4-6) including alkyl, aryl, and acyl orthophosphate monoesters and phosphorylated proteins (PubMed:10506173, PubMed:15280042, PubMed:20498373, PubMed:9584846).
  Has lipid phosphatase activity and inactivates lysophosphatidic acid in seminal plasma (PubMed:10506173, PubMed:15280042).
  Isoform 2:
  Tyrosine phosphatase that acts as a tumor suppressor of prostate cancer through dephosphorylation of ERBB2 and deactivation of MAPK-mediated signaling (PubMed:20498373).
  In addition to its tyrosine phosphatase activity has ecto-5'-nucleotidase activity in dorsal root ganglion (DRG) neurons. Generates adenosine from AMP which acts as a pain suppressor (By similarity).
  PAPf39:
  (Microbial infection) Forms amyloid beta-sheet fibrils in semen. These fibrils, termed SEVI (semen-derived enhancer of viral infection) capture HIV virions, attach them to target cells and enhance infection (PubMed:18083097, PubMed:19451623, PubMed:19897482).
  SEVI amyloid fibrils are degraded by polyphenol epigallocatechin-3-gallate (EGCG), a constituent of green tea (PubMed:19451623).
  Target cell attachment and enhancement of HIV infection is inhibited by surfen (PubMed:19897482).
  Also similarly boosts XMRV (xenotropic murine leukemia virus-related virus) infection (PubMed:19403677).

Secondary Antibody

• 2RD-A1460-YJ
• Goat Anti-Mouse IgG1 Secondary antibody

• 2RD-A1562-YJ
• Rabbit Anti-Mouse IgG1 Secondary antibody (Clone RM106)

Recommended Dilution Buffer

• MRO-0253YJ
• Reconstitution Buffer PBS

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