Mouse Anti-ACTB Recombinant Antibody (clone 1D12E12B5)
CAT#: ZG-0296U
Mouse anti-ACTB antibody (clone 1D12E12B5) displayed high specificity against the ACTB protein of Human, Mouse, Rat and Rabbit.
Tested Data
Gene Expression
WB
Figure 1 Mouse Anti-ACTB Antibody (ZG-0296U) in WB
Positive WB detected in: 293T whole cell lysate, Hela whole cell lysate, HepG2 whole cell lysate, NIH/3T3 whole cell lysate, K562 whole cell lysate, Rat spleen tissue, Rabbit kidney tissue All lanes: ACTB antibody at 1: 5000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 42 KDa
Observed band size: 42 KDa
Exposure time: 5min
IHC
Figure 2 Mouse Anti-ACTB Antibody (ZG-0296U) in IHC
IHC image of ZG-0296U diluted at 1:500 and staining in paraffin-embedded breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at 37°C Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB.
IF
Figure 3 Mouse Anti-ACTB Antibody (ZG-0296U) in IF
Immunofluorescence staining of Hela cells with ZG-0296U at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Mouse IgG (H+L).
FC
Figure 4 Mouse Anti-ACTB Antibody (ZG-0296U) in FC
Overlay Peak curve showing Hela cells stained with ZG-0296U (red line) at 1:200. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1µg/1*106cells) for 1 h at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4°C. Isotype control antibody (green line) was mouse IgG2b (1µg/1*106cells) used under the same conditions. Acquisition of>10,000 events was performed.
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Normal Tissue
Figure 1 IHC staining of human tonsil
Immunohistochemical staining of human tonsil shows strong cytoplasmic positivity in reaction center cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2621/ihc_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Endothelial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/41271/103139_B_7_5.jpg
Normal Tissue
Figure 3 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Leydig cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2621/6575_A_6_6.jpg
Normal Tissue
Figure 4 Lymph node
Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/41271/103139_A_7_8.jpg
Normal Tissue
Figure 5 Colon
Enterocytes - Microvilli Staining: High Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/41271/103139_A_8_3.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/41271/103139_A_9_5.jpg
RNA Expression
Figure 7 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000075624-ACTB
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Specifications
- Immunogen
- A synthesized peptide derived from human Beta-Actin (1-50AA)
- Host Species
- Mouse
- Type
- Mouse IgG2b
- Specificity
- ACTB
- Species Reactivity
- Human, Mouse, Rat, Rabbit
- Clone
- 1D12E12B5
- Applications
- ELISA, WB, IHC, IF, FC, IP
- Conjugate
- Unconjugated
- Related Disease
- Dystonia, Juvenile-Onset and Baraitser-Winter Syndrome 1.
Product Property
- Purification
- Protein A purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- PBS, pH 7.4, 0.03% Proclin 300, 50% glycerol.
- Preservative
- 0.03% Proclin 300
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry, Immunoprecipitation. Specific conditions should be optimized by the end user.
WB:1:5000-1:80000, IHC: 1:500-1:1000, IF: 1:50-1:200, FC: 1:100-1:300, IP: 1ul-5ul
Target
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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