Mouse Anti-ACTB Recombinant Antibody (clone 1D12E12B5) (CAT#: ZG-0296U)

Figure 1 Mouse Anti-ACTB Antibody (ZG-0296U) in WB

Positive WB detected in: 293T whole cell lysate, Hela whole cell lysate, HepG2 whole cell lysate, NIH/3T3 whole cell lysate, K562 whole cell lysate, Rat spleen tissue, Rabbit kidney tissue All lanes: ACTB antibody at 1: 5000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 42 KDa
Observed band size: 42 KDa
Exposure time: 5min

Figure 2 Mouse Anti-ACTB Antibody (ZG-0296U) in IHC

IHC image of ZG-0296U diluted at 1:500 and staining in paraffin-embedded breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at 37°C Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB.

Figure 3 Mouse Anti-ACTB Antibody (ZG-0296U) in IF

Immunofluorescence staining of Hela cells with ZG-0296U at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Mouse IgG (H+L).

Figure 4 Mouse Anti-ACTB Antibody (ZG-0296U) in FC

Overlay Peak curve showing Hela cells stained with ZG-0296U (red line) at 1:200. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1µg/1*106cells) for 1 h at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4°C. Isotype control antibody (green line) was mouse IgG2b (1µg/1*106cells) used under the same conditions. Acquisition of>10,000 events was performed.