Mouse Anti-Actin, smooth muscle (SMA) Recombinant Antibody (ZG-0015J)
CAT#: ZG-0015J
This product is a mouse antibody that recognizes Actin, smooth muscle (SMA).
Tested Data
IHC
Figure 1 Mouse Anti-Actin, smooth muscle (SMA) Recombinant Antibody (ZG-0015J) in IHC
Immunohistochemical analysis of paraffin-embedded Cardiac muscle. 1. Antibody was diluted at 1:200 (4°C overnight). 2, TRIS-EDTA of pH8.0 was used for antigen retrieval. 3, Secondary Antibody was diluted at 1:200 (room temperature, 30min)
IHC
Figure 2 Mouse Anti-Actin, smooth muscle (SMA) Recombinant Antibody (ZG-0015J) in IHC
Immunohistochemical analysis of paraffin-embedded Cardiac muscle. 1. Antibody was diluted at 1:200 (4°C overnight). 2, TRIS-EDTA of pH8.0 was used for antigen retrieval. 3, Secondary Antibody was diluted at 1:200 (room temperature, 30min)
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Specifications
- Immunogen
- Synthesized peptide derived from human Actin, smooth muscle (SMA)
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human Actin, smooth muscle (SMA)
- Species Reactivity
- Human, Mouse
- Applications
- IHC-P, IF (paraffin section)
Product Property
- Storage
- Store at 4°C for short term. For long term storage, store at-20°C, avoiding freeze/thaw cycles.
Applications
- Application Notes
- The antibody was validated for Immunohistochemistry-Paraffin, Immunofluorescence (paraffin section).
Target
- Alternative Names
- Actin, smooth muscle (SMA); Actin; Actin, smooth muscle
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Clean IHC-P Staining
We used this mouse anti-SMA antibody for immunohistochemistry on paraffin-embedded tissue sections. It produced crisp staining of smooth muscle actin in human samples. The antibody clearly delineates the muscle structure, which is essential for our research on mesenchymal cells.
Works For IF As Well
In addition to IHC, we validated this antibody for immunofluorescence on paraffin sections. The signal was strong and specific for Actin, smooth muscle (SMA). It shows good reactivity with both human and mouse tissues, making it a flexible reagent for our lab.
Q&As
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What species does this antibody react with?
A: This Mouse Anti-Actin, smooth muscle (SMA) Recombinant Antibody reacts with both Human and Mouse samples. It recognizes the SMA protein.
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What was the immunogen used to generate this antibody?
A: The antibody was generated using a synthesized peptide derived from human Actin, smooth muscle (SMA). It is validated for IHC-P and IF.
View the frequently asked questions answered by Creative Biolabs Support.
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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