Mouse Anti-ACTN1 Recombinant Antibody (clone 4A12) (CAT#: VS3-CJ617)
This product is a mouse antibody that recognizes human, mouse, and rat ACTN1.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & focal adhesion sites.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4303/669_H5_2_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & focal adhesion sites.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4303/669_H5_2_selected.jpg
Figure 2 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to actin filaments.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/6035/if_selected.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to actin filaments.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/6035/if_selected.jpg
Figure 3 Testis
(Elongated or late spermatids Staining: Medium Intensity: Moderate Quantity:>75% Pachytene spermatocytes Staining: Medium Intensity: Moderate Quantity:>75% Round or early spermatids Staining: Medium Intensity: Moderate Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/6035/18297_A_6_6.jpg
(Elongated or late spermatids Staining: Medium Intensity: Moderate Quantity:>75% Pachytene spermatocytes Staining: Medium Intensity: Moderate Quantity:>75% Round or early spermatids Staining: Medium Intensity: Moderate Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/6035/18297_A_6_6.jpg
Figure 4 Smooth muscle
(Smooth muscle cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/6035/18495_B_5_7.jpg
(Smooth muscle cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/6035/18495_B_5_7.jpg
Figure 5 Lymph node
(Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/6035/18297_A_7_8.jpg
(Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/6035/18297_A_7_8.jpg
Figure 6 Colon
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/6035/18297_A_8_3.jpg
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/6035/18297_A_8_3.jpg
Figure 7 Liver
(Cholangiocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/6035/18297_A_8_4.jpg
(Cholangiocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/6035/18297_A_8_4.jpg
Figure 8 Kidney
(Cells in glomeruli Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/6035/18297_A_9_5.jpg
(Cells in glomeruli Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/6035/18297_A_9_5.jpg
Figure 9 Cerebral cortex
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/6035/18495_B_8_5.jpg
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/6035/18495_B_8_5.jpg
Figure 10 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000072110-ACTN1
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000072110-ACTN1
Specifications
- Immunogen
- Recombinant protein of a-actinin of ACTN1
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human, Mouse, Rat ACTN1
- Species Reactivity
- Human, Mouse, Rat
- Clone
- 4A12
- Applications
- IF, IHC
- Conjugate
- Unconjugated
Product Property
- Purification
- Immunogen affinity chromatography
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Concentration
- 1 mg/mL (lot specific)
- Buffer
- 50% Glycerol, 0.5% BSA, PBS.
- Preservative
- 0.02% Sodium Azide
- Storage
- Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Immunofluorescence, Immunohistochemistry (1:100-1:200).
Target
- Alternative Names
- BDPLT15
- Gene ID
- 87
- UniProt ID
- P12814
- Sequence Similarities
- Belongs to the alpha-actinin family.
- Cellular Localization
- Cell junction, Cell membrane, Cell projection, Cytoplasm, Cytoskeleton, Membrane
- Protein Refseq
- NP_001093.1; NP_001123476.1; NP_001123477.1
- Function
- F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein.
Recommended Products
Isotype Control
Secondary Antibody
Recommended Dilution Buffer
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "ACTN1"
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3334z | Mouse Anti-ACTN1 Recombinant Antibody (clone 1H2) | WB, ELISA, FC | Mouse IgG2a, κ |
| MOB-0593MZ | Mouse Anti-ACTN1 Recombinant Antibody (clone 0.U.03) | IF, IHC, WB | Mouse IgG1 |
| MRO-1640-CN | Rabbit Anti-ACTN1 Polyclonal Antibody (MRO-1640-CN) | WB, IF, IHC, FC | Rabbit IgG |
| VS3-QX21 | Mouse Anti-ACTN1 Recombinant Antibody (clone 1A5-7D1-9H10) | IHC | Mouse IgG1 |
| VS4-WK17 | Mouse Anti-ACTN1 Recombinant Antibody (VS4-WK17) | ELISA, IHC | Mouse IgG |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0054 | Hi-Affi™ Rabbit Anti-ACTN1 Recombinant Antibody (clone DS54AB) | FC, IF, IP, WB | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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