Rabbit Anti-ACTR1B Recombinant Antibody (clone R05-1E3)

CAT#: VS3-FY1965

This rabbit recombinant antibody is specifically engineered to detect human ACTR1B. It is suitable for use in a variety of assays, including WB, IHC-F, IHC-P, ICC/IF, IP. We verify the QC result of the antibody through western blot (dilution 1/500-1/1000), immunohistochemistry (dilution 1/50-1/100), immunofluorescence (dilution 1/50-1/200), immunoprecipitation (dilution 1/20).

Tested Data
Figure 1 Anti-ACTR1B Antibody (VS3-FY1965) in ICC. Figure 2 Anti-ACTR1B Antibody (VS3-FY1965) in WB.

Specifications

  • Immunogen
  • Synthetic peptide of human ACTR1B
  • Host Species
  • Rabbit
  • Type
  • Rabbit IgG
  • Specificity
  • Human ACTR1B
  • Species Reactivity
  • Human
  • Clone
  • R05-1E3
  • Applications
  • Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunofluorescence, Immunoprecipitation
  • MW
  • The calculated weight of the target protein is approximately 42 kDa, while the observed molecular weight stands at 42 kDa.
  • Related Disease
  • Brachydactyly, Type A3 and Alcoholic Psychosis

Product Property

  • Purification
  • Affinity Purified
  • Format
  • Liquid
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Buffer
  • 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.05% BSA
  • Preservative
  • 0.01% Sodium azide
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • Western Blot: 1/500-1/1000
    Immunohistochemistry: 1/50-1/100
    Immunofluorescence: 1/50-1/200
    Immunoprecipitation: 1/20

Target

  • Alternative Names
  • ARP1 Actin Related Protein 1 Homolog B; ARP1 Actin-Related Protein 1 Homolog B, Centractin Beta; Actin-Related Protein 1B; Centractin Beta; CTRN2; ARP1B; ARP1 (Actin-Related Protein 1, Yeast) Homolog B (Centractin Beta); Beta-Centractin; PC3
  • Sequence Similarities
  • Belongs to the actin family. ARP1 subfamily.
  • Cellular Localization
  • Cytoplasm, Cytoskeleton
  • Post Translation Modifications
  • Modification sites at PhosphoSitePlus (P42025)
    Modification sites at neXtProt (NX_P42025)
    Glycosylation from GlyGen (P42025) 1 site, 1 O-linked glycan (1 site)
  • Function
  • Component of a multi-subunit complex involved in microtubule based vesicle motility. It is associated with the centrosome.
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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

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Protocol & Troubleshooting

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

Isotype Control

Secondary Antibody

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VS-0525-XY127 Anti-ACTR1B Immunohistochemistry Kit IHC
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