Rabbit Anti-AGPS Recombinant Antibody (clone R04-3C4) (CAT#: VS3-FY1644)
This is a rabbit recombinant antibody that has the application function of IHC. The antibody is specifically engineered to detect human AGPS. Western blot demonstrated that R04-3C4 recognized a target protein with a molecular weight of 73 kDa.
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to peroxisomes.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/30209/320_H7_1_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to peroxisomes.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/30209/320_H7_1_selected.jpg
Figure 2 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoli & peroxisomes.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/30211/560_D9_1_selected.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoli & peroxisomes.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/30211/560_D9_1_selected.jpg
Figure 3 IHC staining of human caudate
(Immunohistochemical staining of human caudate shows strong cytoplasmic positivity in neuronal cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/30209/ihc_selected.jpg
(Immunohistochemical staining of human caudate shows strong cytoplasmic positivity in neuronal cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/30209/ihc_selected.jpg
Figure 4 IHC staining of human pancreas
(Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in subsets of islet cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/30211/ihc_selected.jpg
(Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in subsets of islet cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/30211/ihc_selected.jpg
Figure 5 Testis
(Sertoli cells Staining: Medium Intensity: Moderate Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/30210/66883_A_4_6.jpg
(Sertoli cells Staining: Medium Intensity: Moderate Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/30210/66883_A_4_6.jpg
Figure 6 Epididymis
(Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/30210/66883_A_1_6.jpg
(Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/30210/66883_A_1_6.jpg
Figure 7 Colon
(Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/30210/66883_A_8_3.jpg
(Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/30210/66883_A_8_3.jpg
Figure 8 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000018510-AGPS
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000018510-AGPS
Specifications
- Immunogen
- Recombinant human alkyl-DHAP synthase protein
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human AGPS
- Species Reactivity
- Human
- Clone
- R04-3C4
- Applications
- Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation
- MW
- The calculated weight of the target protein is approximately 73 kDa, while the observed molecular weight stands at 73 kDa.
- Related Disease
- Rhizomelic Chondrodysplasia Punctata, Type 3 and Rhizomelic Chondrodysplasia Punctata
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Immunoprecipitation: 1/20
Target
- Alternative Names
- Alkylglycerone Phosphate Synthase; Aging-Associated Gene 5 Protein; Alkyl-DHAP Synthase; EC 2.5.1.26; Alkyldihydroxyacetonephosphate Synthase, Peroxisomal; Alkylglycerone-Phosphate Synthase; Aging-Associated Protein 5
- Gene ID
- 8540
- UniProt ID
- O00116
- Sequence Similarities
- Belongs to the FAD-binding oxidoreductase/transferase type 4 family.
- Cellular Localization
- Membrane, Peroxisome
- Post Translation Modifications
- Acetylation, Phosphoprotein
- Protein Refseq
- NP_003650.1
- Function
- Catalyzes the exchange of the acyl chain in acyl-dihydroxyacetonephosphate (acyl-DHAP) for a long chain fatty alcohol, yielding the first ether linked intermediate, i.e. alkyl-dihydroxyacetonephosphate (alkyl-DHAP), in the pathway of ether lipid biosynthesis.
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IgG Antibodies
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2236z | Mouse Anti-AGPS Recombinant Antibody (clone 21B10) | WB, ELISA, IF, IP | Mouse IgG1, κ |
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