Rabbit Anti-AHSG Recombinant Antibody (clone R03-5A4)
CAT#: VS3-FY1717
The R03-5A4 recombinant monoclonal antibody that specifically targets human AHSG protein, a calculated weight of 39 kDa. It cross-reacts with mouse.
The following recommendations serve as a starting point for assay optimization. The actual working concentration may vary and should be determined by the user. The R03-5A4 is recommended for detection of target protein by western blotting (dilution range 1:500-1:1000), immunoprecipitation (dilution range 1:20).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line Hep G2
Immunofluorescent staining of human cell line Hep G2 shows localization to the Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1524/1662_G1_1_selected.jpg
Normal Tissue
Figure 2 IHC staining of human blood vessel
Immunohistochemical staining of human blood vessel shows strong positivity of blood plasma.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/26209/ihc_selected.jpg
Normal Tissue
Figure 3 Liver
Hepatocytes Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1525/4384_A_8_4.jpg
Normal Tissue
Figure 4 Kidney
Cells in tubules Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1525/4384_A_9_5.jpg
Normal Tissue
Figure 5 Bone marrow
Hematopoietic cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1525/4384_B_5_4.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enriched (Hep G2)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000145192-AHSG
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Specifications
- Immunogen
- Synthetic peptide of human fetuin A
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Mouse AHSG
- Species Reactivity
- Human, Mouse
- Clone
- R03-5A4
- Applications
- Western Blot, Immunoprecipitation
- MW
- The calculated weight of the target protein is approximately 39 kDa, while the observed molecular weight stands at 45-50 kDa.
- Related Disease
- Alopecia-Intellectual Disability Syndrome 1 and Alopecia-Intellectual Disability Syndrome
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunoprecipitation: 1/20
Target
- Alternative Names
- Alpha 2-HS Glycoprotein; Ba-Alpha-2-Glycoprotein; Alpha-2-Z-Globulin; Fetuin-A; FETUA; Alpha-2-HS-Glycoprotein
- Gene ID
- 197
- UniProt ID
- P02765
- Sequence Similarities
- Belongs to the fetuin family.
- Cellular Localization
- Secreted
- Post Translation Modifications
- Phosphorylated by FAM20C in the extracellular medium.
- Protein Refseq
- NP_001341500.1; NP_001341501.1; NP_001341502.1; NP_001613.2
- Function
- Promotes endocytosis, possesses opsonic properties and influences the mineral phase of bone. Shows affinity for calcium and barium ions.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "AHSG"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1879z | Mouse Anti-AHSG Recombinant Antibody (clone 25A9) | ELISA, WB, IP, FuncS | Mouse IgG1 |
| MOB-0588MZ | Mouse Anti-AHSG Recombinant Antibody (clone 26) | ELISA, WB | Mouse IgG1 |
| MOB-262YJL | Rabbit Anti-AHSG Recombinant Antibody (clone CBA247) | ELISA | Rabbit IgG |
| ZG-0162U | Mouse Anti-AHSG Recombinant Antibody (clone 4A1B5) | ELISA, IHC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0109 | Hi-Affi™ Rabbit Anti-AHSG Recombinant Antibody (clone DS109AB) | WB, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY66 | Anti-AHSG Immunohistochemistry Kit | IHC | |
| VS-0525-XY238 | Anti-Human AHSG Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0625-YC9 | Recombinant Anti-AHSG Eliminating Antibody, pH-Sensitive (CBA247 var.) | Antigen-Sweeping In Vivo. | Rabbit IgG |
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