Rabbit Anti-AHSG Recombinant Antibody (clone R03-5A4) (CAT#: VS3-FY1717)
The R03-5A4 recombinant monoclonal antibody that specifically targets human AHSG protein, a calculated weight of 39 kDa. It cross-reacts with mouse.
The following recommendations serve as a starting point for assay optimization. The actual working concentration may vary and should be determined by the user. The R03-5A4 is recommended for detection of target protein by western blotting (dilution range 1:500-1:1000), immunoprecipitation (dilution range 1:20).
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line Hep G2
(Immunofluorescent staining of human cell line Hep G2 shows localization to the Golgi apparatus.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1524/1662_G1_1_selected.jpg
(Immunofluorescent staining of human cell line Hep G2 shows localization to the Golgi apparatus.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1524/1662_G1_1_selected.jpg
Figure 2 IHC staining of human blood vessel
(Immunohistochemical staining of human blood vessel shows strong positivity of blood plasma.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/26209/ihc_selected.jpg
(Immunohistochemical staining of human blood vessel shows strong positivity of blood plasma.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/26209/ihc_selected.jpg
Figure 3 Liver
(Hepatocytes Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1525/4384_A_8_4.jpg
(Hepatocytes Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1525/4384_A_8_4.jpg
Figure 4 Kidney
(Cells in tubules Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1525/4384_A_9_5.jpg
(Cells in tubules Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1525/4384_A_9_5.jpg
Figure 5 Bone marrow
(Hematopoietic cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1525/4384_B_5_4.jpg
(Hematopoietic cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1525/4384_B_5_4.jpg
Figure 6 RNA cell line category: Cell line enriched (Hep G2)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000145192-AHSG
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000145192-AHSG
Specifications
- Immunogen
- Synthetic peptide of human fetuin A
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Mouse AHSG
- Species Reactivity
- Human, Mouse
- Clone
- R03-5A4
- Applications
- Western Blot, Immunoprecipitation
- MW
- The calculated weight of the target protein is approximately 39 kDa, while the observed molecular weight stands at 45-50 kDa.
- Related Disease
- Alopecia-Intellectual Disability Syndrome 1 and Alopecia-Intellectual Disability Syndrome
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunoprecipitation: 1/20
Target
- Alternative Names
- Alpha 2-HS Glycoprotein; Ba-Alpha-2-Glycoprotein; Alpha-2-Z-Globulin; Fetuin-A; FETUA; Alpha-2-HS-Glycoprotein
- Gene ID
- 197
- UniProt ID
- P02765
- Sequence Similarities
- Belongs to the fetuin family.
- Cellular Localization
- Secreted
- Post Translation Modifications
- Phosphorylated by FAM20C in the extracellular medium.
- Protein Refseq
- NP_001341500.1; NP_001341501.1; NP_001341502.1; NP_001613.2
- Function
- Promotes endocytosis, possesses opsonic properties and influences the mineral phase of bone. Shows affinity for calcium and barium ions.
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "AHSG"
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1879z | Mouse Anti-AHSG Recombinant Antibody (clone 25A9) | ELISA, WB, IP, FuncS | Mouse IgG1 |
| MOB-0588MZ | Mouse Anti-AHSG Recombinant Antibody (clone 26) | ELISA, WB | Mouse IgG1 |
| MOB-262YJL | Rabbit Anti-AHSG Recombinant Antibody (clone CBA247) | ELISA | Rabbit IgG |
| ZG-0162U | Mouse Anti-AHSG Recombinant Antibody (clone 4A1B5) | ELISA, IHC | Mouse IgG1 |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0109 | Hi-Affi™ Rabbit Anti-AHSG Recombinant Antibody (clone DS109AB) | WB, IP | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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