Rabbit Anti-AIFM1 Recombinant Antibody (VS3-CJ194)
CAT#: VS3-CJ194
This product is a rabbit antibody that recognizes AIFM1.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/30611/1912_B2_32_selected.jpg
Normal Tissue
Figure 2 Kidney
Bowman's capsule Staining: High Intensity: Strong Quantity: 75%-25% Collecting ducts Staining: Medium Intensity: Strong Quantity: <25% Distal tubules Staining: High Intensity: Strong Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3764/10710_A_9_5.jpg
Normal Tissue
Figure 3 Testis
Leydig cells Staining: High Intensity: Strong Quantity:>75% Sertoli cells Staining: High Intensity: Strong Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3764/10710_A_5_6.jpg
Normal Tissue
Figure 4 Adrenal gland
Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3764/10710_B_4_5.jpg
Normal Tissue
Figure 5 Liver
Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3764/10710_A_9_4.jpg
RNA Expression
Figure 6 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000156709-AIFM1
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Specifications
- Immunogen
- Recombinant protein
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat AIFM1
- Species Reactivity
- Human, Mouse, Rat
- Applications
- WB, ICC, IHC, IP, FC
- Conjugate
- Unconjugated
Product Property
- Purification
- Protein A affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- 40% Glycerol, 1% BSA, TBS, pH7.4.
- Preservative
- 0.05% sodium azide
- Storage
- Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- This antibody has been tested for use in Western Blot, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Flow Cytometry.
Target
- Alternative Names
- AIF; CMT2D; CMTX4; COWCK; COXPD6; NADMR; NAMSD; PDCD8; DFNX5; apoptosis inducing factor; mitochondria associated 1; apoptosis inducing factor mitochondria associated 1; AUNX1; SEMDHL
- Gene ID
- 3131
- UniProt ID
- O95831
- Sequence Similarities
- Belongs to the FAD-dependent oxidoreductase family.
- Cellular Localization
- Cytoplasm, Membrane, Mitochondrion, Mitochondrion inner membrane, Nucleus
- Post Translation Modifications
- Under normal conditions, a 54-residue N-terminal segment is first proteolytically removed during or just after translocation into the mitochondrial intermembrane space (IMS) by the mitochondrial processing peptidase (MPP) to form the inner-membrane-anchored mature form (AIFmit). During apoptosis, it is further proteolytically processed at amino-acid position 101 leading to the generation of the mature form, which is confined to the mitochondrial IMS in a soluble form (AIFsol). AIFsol is released to the cytoplasm in response to specific death signals, and translocated to the nucleus, where it induces nuclear apoptosis in a caspase-independent manner.
Ubiquitination by XIAP/BIRC4 does not lead to proteasomal degradation. Ubiquitination at Lys-255 by XIAP/BIRC4 blocks its ability to bind DNA and induce chromatin degradation, thereby inhibiting its ability to induce cell death.
- Protein Refseq
- NP_001124318.2; NP_001124319.1; NP_004199.1
- Function
- Functions both as NADH oxidoreductase and as regulator of apoptosis (PubMed:20362274, PubMed:23217327, PubMed:17094969, PubMed:33168626).
In response to apoptotic stimuli, it is released from the mitochondrion intermembrane space into the cytosol and to the nucleus, where it functions as a proapoptotic factor in a caspase-independent pathway (PubMed:20362274).
Release into the cytoplasm is mediated upon binding to poly-ADP-ribose chains (By similarity).
The soluble form (AIFsol) found in the nucleus induces 'parthanatos' i.e. caspase-independent fragmentation of chromosomal DNA (PubMed:20362274).
Binds to DNA in a sequence-independent manner (PubMed:27178839).
Interacts with EIF3G, and thereby inhibits the EIF3 machinery and protein synthesis, and activates caspase-7 to amplify apoptosis (PubMed:17094969).
Plays a critical role in caspase-independent, pyknotic cell death in hydrogen peroxide-exposed cells (PubMed:19418225).
In contrast, participates in normal mitochondrial metabolism. Plays an important role in the regulation of respiratory chain biogenesis by interacting with CHCHD4 and controlling CHCHD4 mitochondrial import (PubMed:26004228).
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "AIFM1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2902z | Mouse Anti-AIFM1 Recombinant Antibody (clone 28D2) | ELISA, WB, ICC, FC, IF | Mouse IgG2a, κ |
| MRO-0038-CN | Rabbit Anti-AIFM1 Recombinant Antibody (clone CBACN-014) | WB, IF, IHC, IP, FC | Rabbit IgG |
| ZG-0267F | Mouse Anti-AIFM1 Recombinant Antibody (ZG-0267F) | WB, ICC | Mouse IgG |
| ZG-0537J | Rabbit Anti-AIFM1 Recombinant Antibody (clone 4B2) | ELISA, WB, IHC | Rabbit IgG |
| VS3-QX25 | Mouse Anti-AIFM1 Recombinant Antibody (clone 8H1-B10-A12) | WB, IF | Mouse IgG2a |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0112 | Hi-Affi™ Rabbit Anti-AIFM1 Recombinant Antibody (clone DS112AB) | WB, IP, IHC, ICC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY9 | AbPlus™ Anti-AIFM1 Magnetic Beads (VS-0424-XY9) | IP, Protein Purification | |
| VS-0724-YC1250 | AbPlus™ Anti-AIFM1 Magnetic Beads (VS-0724-YC1250) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY67 | Anti-AIFM1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY244 | Anti-Mouse AIFM1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY243 | Anti-Human AIFM1 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC35 | CytoStream™ Rabbit Anti-AIFM1 Recombinant Antibody (VS13-YC35) | WB, ICC, IF, IHC-P, IP, FC | Rabbit IgG |
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