Rabbit Anti-AKAP8 Recombinant Antibody (clone R05-7H8) (CAT#: VS3-FY1967)
The R05-7H8 recombinant monoclonal antibody specifically targets human AKAP8. It cross-reacts with mouse, rat. It is suitable for use in a variety of assays, including WB, IHC-F, IHC-P, ICC/IF. Applications Tested: The AKAP95 antibody has been tested by western blot analysis of AKAP 95 in PC-12, 3T3 lysates and western blot analysis of AKAP 95 in Hela, A549, HL-60, U251 lysates.
Specific Inquiry
WB
Figure 1 Anti-AKAP8 Antibody (VS3-FY1967) in WB.
The AKAP 95 expression in PC-12, 3T3 lysates were detected using the anti-AKAP95 by western blot assay.
WB
Figure 2 Anti-AKAP8 Antibody (VS3-FY1967) in WB.
The AKAP 95 expression in Hela, A549, HL-60, U251 lysates were detected using the anti-AKAP 95 by western blot assay.
Specifications
- Immunogen
- Synthetic peptide of human AKAP 95
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat AKAP8
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R05-7H8
- Applications
- Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunofluorescence
- MW
- The calculated weight of the target protein is approximately 76 kDa, while the observed molecular weight stands at 95 kDa.
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Immunofluorescence: 1/50-1/200
Target
- Alternative Names
- A-Kinase Anchoring Protein 8; A Kinase (PRKA) Anchor Protein 8; A-Kinase Anchor Protein, 95kDa; AKAP 95; AKAP-8; AKAP95; A-Kinase Anchor Protein 95 KDa; A-Kinase Anchor Protein 8; AKAP-95
- Gene ID
- 10270
- UniProt ID
- O43823
- Sequence Similarities
- Belongs to the AKAP95 family.
- Cellular Localization
- Cytoplasm, Nucleus
- Post Translation Modifications
- Phosphorylated on tyrosine residues probably by SRC subfamily protein kinases; multiple phosphorylation is leading to dissociation from nuclear structures implicated in chromatin structural changes. (O43823-AKAP8_HUMAN)
Modification sites at PhosphoSitePlus (O43823)
Modification sites at neXtProt (NX_O43823)
Glycosylation from GlyGen (O43823) 5 sites, 2 O-linked glycans (5 sites)
- Protein Refseq
- NP_005849.1
- Function
- Acts as an anchor for a PKA-signaling complex onto mitotic chromosomes, which is required for maintenance of chromosomes in a condensed form throughout mitosis. Recruits condensin complex subunit NCAPD2 to chromosomes required for chromatin condensation; the function appears to be independent from PKA-anchoring.
Recommended Products
Isotype Control
Secondary Antibody
Related Resources
cAMP Signaling Pathway
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "AKAP8"
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IgG Antibody Products
Rabbit Monoclonal Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0543MZ | Mouse Anti-AKAP8 Recombinant Antibody (clone 3026D2) | WB | Mouse IgG1 |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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