Rabbit Anti-AKAP8 Recombinant Antibody (clone R05-7H8) (CAT#: VS3-FY1967)
The R05-7H8 recombinant monoclonal antibody specifically targets human AKAP8. It cross-reacts with mouse, rat. It is suitable for use in a variety of assays, including WB, IHC-F, IHC-P, ICC/IF. Applications Tested: The AKAP95 antibody has been tested by western blot analysis of AKAP 95 in PC-12, 3T3 lysates and western blot analysis of AKAP 95 in Hela, A549, HL-60, U251 lysates.
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WB
Figure 1 Anti-AKAP8 Antibody (VS3-FY1967) in WB.
The AKAP 95 expression in PC-12, 3T3 lysates were detected using the anti-AKAP95 by western blot assay.
WB
Figure 2 Anti-AKAP8 Antibody (VS3-FY1967) in WB.
The AKAP 95 expression in Hela, A549, HL-60, U251 lysates were detected using the anti-AKAP 95 by western blot assay.
Specifications
- Immunogen
- Synthetic peptide of human AKAP 95
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat AKAP8
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R05-7H8
- Applications
- Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunofluorescence
- MW
- The calculated weight of the target protein is approximately 76 kDa, while the observed molecular weight stands at 95 kDa.
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Immunofluorescence: 1/50-1/200
Target
- Alternative Names
- A-Kinase Anchoring Protein 8; A Kinase (PRKA) Anchor Protein 8; A-Kinase Anchor Protein, 95kDa; AKAP 95; AKAP-8; AKAP95; A-Kinase Anchor Protein 95 KDa; A-Kinase Anchor Protein 8; AKAP-95
- Gene ID
- 10270
- UniProt ID
- O43823
- Sequence Similarities
- Belongs to the AKAP95 family.
- Cellular Localization
- Cytoplasm, Nucleus
- Post Translation Modifications
- Phosphorylated on tyrosine residues probably by SRC subfamily protein kinases; multiple phosphorylation is leading to dissociation from nuclear structures implicated in chromatin structural changes. (O43823-AKAP8_HUMAN)
Modification sites at PhosphoSitePlus (O43823)
Modification sites at neXtProt (NX_O43823)
Glycosylation from GlyGen (O43823) 5 sites, 2 O-linked glycans (5 sites)
- Protein Refseq
- NP_005849.1
- Function
- Acts as an anchor for a PKA-signaling complex onto mitotic chromosomes, which is required for maintenance of chromosomes in a condensed form throughout mitosis. Recruits condensin complex subunit NCAPD2 to chromosomes required for chromatin condensation; the function appears to be independent from PKA-anchoring.
Recommended Products
Isotype Control
Secondary Antibody
Related Resources
cAMP Signaling Pathway
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IgG Antibody Products
Rabbit Monoclonal Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0543MZ | Mouse Anti-AKAP8 Recombinant Antibody (clone 3026D2) | WB | Mouse IgG1 |
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