Rabbit Anti-AKAP8 Recombinant Antibody (clone R05-7H8)
CAT#: VS3-FY1967
The R05-7H8 recombinant monoclonal antibody specifically targets human AKAP8. It cross-reacts with mouse, rat. It is suitable for use in a variety of assays, including WB, IHC-F, IHC-P, ICC/IF. Applications Tested: The AKAP95 antibody has been tested by western blot analysis of AKAP 95 in PC-12, 3T3 lysates and western blot analysis of AKAP 95 in Hela, A549, HL-60, U251 lysates.
Tested Data
Gene Expression
WB
Figure 1 Anti-AKAP8 Antibody (VS3-FY1967) in WB.
The AKAP 95 expression in PC-12, 3T3 lysates were detected using the anti-AKAP95 by western blot assay.
WB
Figure 2 Anti-AKAP8 Antibody (VS3-FY1967) in WB.
The AKAP 95 expression in Hela, A549, HL-60, U251 lysates were detected using the anti-AKAP 95 by western blot assay.
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Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4776/68_E2_2_selected.jpg
Normal Tissue
Figure 2 IHC staining of human pancreas
Immunohistochemical staining of human pancreas shows strong nuclear positivity in exocrine glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4776/155098_A_1_3_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Endothelial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear Glial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: nuclear Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4776/155098_B_7_5.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4776/155098_A_9_3.jpg
Normal Tissue
Figure 5 Liver
Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4776/155098_A_9_4.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity:>75% Location: nuclear Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4776/155098_A_9_5.jpg
Normal Tissue
Figure 7 Testis
Leydig cells Staining: High Intensity: Strong Quantity:>75% Pachytene spermatocytes Staining: Medium Intensity: Moderate Quantity:>75% Peritubular cells Staining: High Intensity: Strong Quantity: 75%-25% Preleptotene spermatocytes Staining: High Intensity: Strong Quantity: 75%-25% Round or early spermatids Staining: Medium Intensity: Strong Quantity: <25% Sertoli cells Staining: High Intensity: Strong Quantity:>75% Spermatogonia cells Staining: High Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4776/155098_A_5_6.jpg
Normal Tissue
Figure 8 Skin
Cells in basal layer Staining: High Intensity: Strong Quantity:>75% Cells in granular layer Staining: High Intensity: Strong Quantity:>75% Cells in spinous layer Staining: High Intensity: Strong Quantity:>75% Endothelial cells Staining: High Intensity: Strong Fibrohistiocytic cells Staining: High Intensity: Strong Langerhans cells Staining: High Intensity: Strong Quantity:>75% Lymphocytes Staining: High Intensity: Strong Melanocytes Staining: High Intensity: Strong Quantity:>75% Vascular mural cells Staining: High Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4776/155098_B_5_8.jpg
Normal Tissue
Figure 9 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4776/155098_A_8_8.jpg
RNA Expression
Figure 10 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000105127-AKAP8
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Specifications
- Immunogen
- Synthetic peptide of human AKAP 95
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat AKAP8
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R05-7H8
- Applications
- Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunofluorescence
- MW
- The calculated weight of the target protein is approximately 76 kDa, while the observed molecular weight stands at 95 kDa.
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Immunofluorescence: 1/50-1/200
Target
- Alternative Names
- A-Kinase Anchoring Protein 8; A Kinase (PRKA) Anchor Protein 8; A-Kinase Anchor Protein, 95kDa; AKAP 95; AKAP-8; AKAP95; A-Kinase Anchor Protein 95 KDa; A-Kinase Anchor Protein 8; AKAP-95
- Gene ID
- 10270
- UniProt ID
- O43823
- Sequence Similarities
- Belongs to the AKAP95 family.
- Cellular Localization
- Cytoplasm, Nucleus
- Post Translation Modifications
- Phosphorylated on tyrosine residues probably by SRC subfamily protein kinases; multiple phosphorylation is leading to dissociation from nuclear structures implicated in chromatin structural changes. (O43823-AKAP8_HUMAN)
Modification sites at PhosphoSitePlus (O43823)
Modification sites at neXtProt (NX_O43823)
Glycosylation from GlyGen (O43823) 5 sites, 2 O-linked glycans (5 sites)
- Protein Refseq
- NP_005849.1
- Function
- Acts as an anchor for a PKA-signaling complex onto mitotic chromosomes, which is required for maintenance of chromosomes in a condensed form throughout mitosis. Recruits condensin complex subunit NCAPD2 to chromosomes required for chromatin condensation; the function appears to be independent from PKA-anchoring.
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Related Signaling Pathways
cAMP Signaling Pathway
Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
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COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "AKAP8"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0543MZ | Mouse Anti-AKAP8 Recombinant Antibody (clone 3026D2) | WB | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0123 | Hi-Affi™ Rabbit Anti-AKAP8 Recombinant Antibody (clone DS123AB) | WB, IHC, ICC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY267 | Anti-AKAP8 Immunohistochemistry Kit | IHC | |
| VS-0525-XY268 | Anti-Mouse AKAP8 Immunohistochemistry Kit | IHC |
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