Rabbit Anti-AKR1C3 Recombinant Antibody (clone 4D12)
CAT#: ZG-0110U
Rabbit anti-AKR1C3 antibody (clone 4D12) displayed high specificity against the AKR1C3 protein of Human.
Tested Data
Gene Expression
IF
Figure 1 Rabbit Anti-AKR1C3 Antibody (ZG-0110U) in IF
Immunofluorescence staining of Hela Cells with ZG-0110U at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).
FC
Figure 2 Rabbit Anti-AKR1C3 Antibody (ZG-0110U) in FC
Overlay histogram showing A549 cells stained with ZG-0110U (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/1*106cells) for 1 h at 4°C.The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4°C. Control antibody (green line) was Rabbit IgG (1µg/1*106cells) used under the same conditions. Acquisition of>10,000 events was performed.
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Normal Tissue
Figure 1 Duodenum
High expression
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10874/25837_A_7_2_val_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear Glial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear Neuronal cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10874/25837_B_9_5.jpg
Normal Tissue
Figure 3 Small intestine
Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10874/25837_A_5_2.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10874/25837_A_7_3.jpg
Normal Tissue
Figure 5 Liver
Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10874/25837_A_8_4.jpg
Normal Tissue
Figure 6 Kidney
Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10874/25837_A_7_5.jpg
Normal Tissue
Figure 7 Testis
Leydig cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10874/25837_A_5_6.jpg
RNA Expression
Figure 8 RNA cell line category: Cell line enhanced (A549, ASC diff, Hep G2, OE19, RT4)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000196139-AKR1C3
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Specifications
- Immunogen
- A synthesized peptide derived from human AKR1C3
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human AKR1C3
- Species Reactivity
- Human
- Clone
- 4D12
- Applications
- ELISA, IF, FC
- Conjugate
- Unconjugated
- Related Disease
- Polycystic Ovary Syndrome and Prostate Disease.
Product Property
- Purification
- Affinity Purified
- Concentration
- See the COA
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, Immunofluorescence, Flow Cytometry.Specific conditions should be optimized by the end user.
IF:1:20-1:200, FC:1:20-1:200
Target
- Alternative Names
- DD3; DDX; PGFS; HAKRB; HAKRe; HA1753; HSD17B5; hluPGFS; aldo-keto reductase family 1 member C3; 3-alpha hydroxysteroid dehydrogenase, type II; 3-alpha-HSD type II, brain; chlordecone reductase homolog HAKRb; dihydrodiol dehydrogenase 3; dihydrodiol dehydrogenase X; indanol dehydrogenase; prostaglandin F synthase; testosterone 17-beta-dehydrogenase 5; trans-1,2-dihydrobenzene-1,2-diol dehydrogenase; type IIb 3-alpha hydroxysteroid dehydrogenase
- Gene ID
- 8644
- UniProt ID
- P42330
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2955z | Mouse Anti-AKR1C3 Recombinant Antibody (clone 35H5) | WB, ICC, IHC | Mouse IgG1 |
| MOB-0563MZ | Mouse Anti-AKR1C3 Recombinant Antibody (clone OQ7.H7.B7) | ELISA, WB | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC38 | CytoStream™ Rabbit Anti-AKR1C3 Recombinant Antibody (VS13-YC38) | WB, ICC, IF, IP, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY276 | Anti-AKR1C3 Immunohistochemistry Kit | IHC |
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