Rabbit Anti-AKR1C3 Recombinant Antibody (clone 4D12) (CAT#: ZG-0110U)
Rabbit anti-AKR1C3 antibody (clone 4D12) displayed high specificity against the AKR1C3 protein of Human.
Specific Inquiry
IF
Figure 1 Rabbit Anti-AKR1C3 Antibody (ZG-0110U) in IF
Immunofluorescence staining of Hela Cells with ZG-0110U at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).
FC
Figure 2 Rabbit Anti-AKR1C3 Antibody (ZG-0110U) in FC
Overlay histogram showing A549 cells stained with ZG-0110U (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/1*106cells) for 1 h at 4°C.The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4°C. Control antibody (green line) was Rabbit IgG (1µg/1*106cells) used under the same conditions. Acquisition of>10,000 events was performed.
Specifications
- Immunogen
- A synthesized peptide derived from human AKR1C3
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human AKR1C3
- Species Reactivity
- Human
- Clone
- 4D12
- Applications
- ELISA, IF, FC
- Conjugate
- Unconjugated
- Related Disease
- Polycystic Ovary Syndrome and Prostate Disease.
Product Property
- Purification
- Affinity Purified
- Concentration
- See the COA
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, Immunofluorescence, Flow Cytometry.Specific conditions should be optimized by the end user.
IF:1:20-1:200, FC:1:20-1:200
Target
- Alternative Names
- DD3; DDX; PGFS; HAKRB; HAKRe; HA1753; HSD17B5; hluPGFS; aldo-keto reductase family 1 member C3; 3-alpha hydroxysteroid dehydrogenase, type II; 3-alpha-HSD type II, brain; chlordecone reductase homolog HAKRb; dihydrodiol dehydrogenase 3; dihydrodiol dehydrogenase X; indanol dehydrogenase; prostaglandin F synthase; testosterone 17-beta-dehydrogenase 5; trans-1,2-dihydrobenzene-1,2-diol dehydrogenase; type IIb 3-alpha hydroxysteroid dehydrogenase
- Gene ID
- 8644
- UniProt ID
- P42330
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2955z | Mouse Anti-AKR1C3 Recombinant Antibody (clone 35H5) | WB, ICC, IHC | Mouse IgG1 |
| MOB-0563MZ | Mouse Anti-AKR1C3 Recombinant Antibody (clone OQ7.H7.B7) | ELISA, WB | Mouse IgG1 |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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