Rabbit Anti-ALOX5 Recombinant Antibody (clone R06-5B9) (CAT#: VS3-XY3258)
It is a recombinant rabbit antibody targeting the human arachidonate 5-lipoxygenase (ALOX5), which regulates bone mineralization and fat cell differentiation increases in induced pluripotent stem cells. This antibody reacts with human, mouse and rat ALOX5. It has been determined using Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Flow cytometry, Immunocytochemistry or Immunofluorescence.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line RT4
(Immunofluorescent staining of human cell line RT4 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/71285/1382_G11_2_selected.jpg
(Immunofluorescent staining of human cell line RT4 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/71285/1382_G11_2_selected.jpg
Figure 2 Spleen
(High expression)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/71285/160734_B_7_4_selected.jpg
(High expression)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/71285/160734_B_7_4_selected.jpg
Figure 3 Cerebral cortex
(Glial cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/71285/160734_B_7_5.jpg
(Glial cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/71285/160734_B_7_5.jpg
Figure 4 Colon
(Enterocytes - Mucosal lymphoid cells Staining: Medium Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/71285/160734_A_7_3.jpg
(Enterocytes - Mucosal lymphoid cells Staining: Medium Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/71285/160734_A_7_3.jpg
Figure 5 Bone marrow
(Hematopoietic cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/71285/160734_B_4_4.jpg
(Hematopoietic cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/71285/160734_B_4_4.jpg
Figure 6 Lymph node
(Germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear Non-germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/71285/160734_A_9_8.jpg
(Germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear Non-germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/71285/160734_A_9_8.jpg
Figure 7 RNA cell line category: Cell line enhanced (BEWO, Daudi, HMC-1, U-266/70, U-698)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000012779-ALOX5
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000012779-ALOX5
Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat ALOX5
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R06-5B9
- Applications
- WB, IHC-F, IHC-P, ICC, IF, FC
- Related Disease
- Asthma; Atherosclerosis Susceptibility
Product Property
- Clonality
- Monoclonal
- Aggregation
- < 5%
- Format
- Liquid
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% glycerol
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IHC: 1/50-1/100
IF: 1/50-1/200
FC: 1/50-1/100
Target
- Alternative Names
- 5-LO; 5LPG; LOG5; 5-LOX
- Gene ID
- 240
- UniProt ID
- P09917
- Long Name
- Arachidonate 5-lipoxygenase
- Cellular Localization
- Cytoplasm, Nucleus, Membrane
- Post Translation Modifications
- Serine phosphorylation by MAPKAPK2 is stimulated by arachidonic acid (PubMed:11844797, PubMed:18978352).
Phosphorylation on Ser-524 by PKA has an inhibitory effect (PubMed:15280375).
Phosphorylation on Ser-272 prevents export from the nucleus (PubMed:11844797, PubMed:18978352).
- Protein Refseq
- XP_047280892.1; XP_047280890.1
- Function
- Catalyzes the oxygenation of arachidonate ((5Z,8Z,11Z,14Z)-eicosatetraenoate) to 5-hydroperoxyeicosatetraenoate (5-HPETE) followed by the dehydration to 5,6- epoxyeicosatetraenoate (Leukotriene A4/LTA4), the first two steps in the biosynthesis of leukotrienes.
Also catalyzes the oxygenation of arachidonate into 8-hydroperoxyicosatetraenoate (8-HPETE) and 12-hydroperoxyicosatetraenoate (12-HPETE).
Recommended Products
Isotype Control
Recommended Dilution Buffer
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "ALOX5"
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2235z | Mouse Anti-ALOX5 Recombinant Antibody (clone 9F9) | WB, IHC | Mouse IgG1 |
| MRO-1621-CN | Rabbit Anti-ALOX5 Polyclonal Antibody (MRO-1621-CN) | WB | Rabbit IgG |
| ZG-0006U | Rabbit Anti-ALOX5 Recombinant Antibody (clone 1D4) | ELISA, IHC | Rabbit IgG |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0152 | Hi-Affi™ Rabbit Anti-ALOX5 Recombinant Antibody (clone DS152AB) | WB, IHC-P, IF | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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