Rabbit Anti-ALOX5 Recombinant Antibody (clone R06-5B9)
CAT#: VS3-XY3258
It is a recombinant rabbit antibody targeting the human arachidonate 5-lipoxygenase (ALOX5), which regulates bone mineralization and fat cell differentiation increases in induced pluripotent stem cells. This antibody reacts with human, mouse and rat ALOX5. It has been determined using Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Flow cytometry, Immunocytochemistry or Immunofluorescence.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line RT4
Immunofluorescent staining of human cell line RT4 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/71285/1382_G11_2_selected.jpg
Normal Tissue
Figure 2 Spleen
High expression
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/71285/160734_B_7_4_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Glial cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/71285/160734_B_7_5.jpg
Normal Tissue
Figure 4 Colon
Enterocytes - Mucosal lymphoid cells Staining: Medium Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/71285/160734_A_7_3.jpg
Normal Tissue
Figure 5 Bone marrow
Hematopoietic cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/71285/160734_B_4_4.jpg
Normal Tissue
Figure 6 Lymph node
Germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear Non-germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/71285/160734_A_9_8.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enhanced (BEWO, Daudi, HMC-1, U-266/70, U-698)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000012779-ALOX5
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Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat ALOX5
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R06-5B9
- Applications
- WB, IHC-F, IHC-P, ICC, IF, FC
- Related Disease
- Asthma; Atherosclerosis Susceptibility
Product Property
- Clonality
- Monoclonal
- Aggregation
- < 5%
- Format
- Liquid
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% glycerol
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IHC: 1/50-1/100
IF: 1/50-1/200
FC: 1/50-1/100
Target
- Alternative Names
- 5-LO; 5LPG; LOG5; 5-LOX
- Gene ID
- 240
- UniProt ID
- P09917
- Long Name
- Arachidonate 5-lipoxygenase
- Cellular Localization
- Cytoplasm, Nucleus, Membrane
- Post Translation Modifications
- Serine phosphorylation by MAPKAPK2 is stimulated by arachidonic acid (PubMed:11844797, PubMed:18978352).
Phosphorylation on Ser-524 by PKA has an inhibitory effect (PubMed:15280375).
Phosphorylation on Ser-272 prevents export from the nucleus (PubMed:11844797, PubMed:18978352).
- Protein Refseq
- XP_047280892.1; XP_047280890.1
- Function
- Catalyzes the oxygenation of arachidonate ((5Z,8Z,11Z,14Z)-eicosatetraenoate) to 5-hydroperoxyeicosatetraenoate (5-HPETE) followed by the dehydration to 5,6- epoxyeicosatetraenoate (Leukotriene A4/LTA4), the first two steps in the biosynthesis of leukotrienes.
Also catalyzes the oxygenation of arachidonate into 8-hydroperoxyicosatetraenoate (8-HPETE) and 12-hydroperoxyicosatetraenoate (12-HPETE).
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "ALOX5"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2235z | Mouse Anti-ALOX5 Recombinant Antibody (clone 9F9) | WB, IHC | Mouse IgG1 |
| MRO-1621-CN | Rabbit Anti-ALOX5 Polyclonal Antibody (MRO-1621-CN) | WB | Rabbit IgG |
| ZG-0006U | Rabbit Anti-ALOX5 Recombinant Antibody (clone 1D4) | ELISA, IHC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0152 | Hi-Affi™ Rabbit Anti-ALOX5 Recombinant Antibody (clone DS152AB) | WB, IHC-P, IF | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY326 | Anti-ALOX5 Immunohistochemistry Kit | IHC | |
| VS-0525-XY327 | Anti-Mouse ALOX5 Immunohistochemistry Kit | IHC | |
| VS-0525-XY328 | Anti-Rat ALOX5 Immunohistochemistry Kit | IHC |
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