Mouse Anti-ALPP Recombinant Antibody (clone H17E2) (CAT#: HPAB-0014-YJ)

This product is a recombinant mouse antibody that recognizes human placental alkaline phosphatase (PLAP). Mab H17E2 is used to evaluate placental-like alkaline phosphatase (PLAP) as a serum marker of testicular germ cell tumours and functions in the treatment or diagnosis of cancer.


Specific Inquiry
  • Size:
  • Conjugation:
  • Endotoxin:
  • Purity:
  • Fc Engineering:
  • Published Data
  • Gene Expression
  • Datasheet
  • MSDS
  • COA
WB

Figure 1 Western blot and immunostaining of lysates prepared from various tumour cell lines. Commercially available standard PLAP (molecular weight 70 kDa) was run in parallel as a positive control.

Figure 1 Western blot and immunostaining of lysates prepared from various tumour cell lines. Commercially available standard PLAP (molecular weight 70 kDa) was run in parallel as a positive control.

Nouri, A. M. E., Torabi‐Pour, N., & Dabare, A. A. N. P. M. (2000). A new highly specific monoclonal antibody against placental alkaline phosphatase: a potential marker for the early detection of testis tumour. BJU international, 86(7), 894-900.

ICC

Figure 2 A competition assay between FITC-conjugated H17E2 and unconjugated ATC2, using Hep2 cells immunocytochemically stained with FITC-H17E2 at 300 mg/mL and ATC2 at different concentrations.

Figure 2 A competition assay between FITC-conjugated H17E2 and unconjugated ATC2, using Hep2 cells immunocytochemically stained with FITC-H17E2 at 300 mg/mL and ATC2 at different concentrations.

Nouri, A. M. E., Torabi‐Pour, N., & Dabare, A. A. N. P. M. (2000). A new highly specific monoclonal antibody against placental alkaline phosphatase: a potential marker for the early detection of testis tumour. BJU international, 86(7), 894-900.

IHC

Figure 3 Non-villous trophoblast staining with H17E2.

Figure 3 Non-villous trophoblast staining with H17E2.

Travers, P., & Bodmer, W. (1984). Preparation and characterization of monoclonal antibodies against placental alkaline phosphatase and other human trophoblast‐associated determinants. International journal of cancer, 33(5), 633-641.

Inhib

Figure 4 Inhibition of alkaline phosphatase activity bound by H17E2.

Figure 4 Inhibition of alkaline phosphatase activity bound by H17E2.

Travers, P., & Bodmer, W. (1984). Preparation and characterization of monoclonal antibodies against placental alkaline phosphatase and other human trophoblast‐associated determinants. International journal of cancer, 33(5), 633-641.


Specifications

  • Host Species
  • Mouse
  • Type
  • Mouse IgG, κ
  • Specificity
  • Human ALPP
  • Species Reactivity
  • Human
  • Clone
  • H17E2
  • Applications
  • IHC, ELISA, WB, ICC, Inhib
  • Related Disease
  • Cancer

Product Property

  • Purity
  • >95% as determined by SDS-PAGE and HPLC analysis
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Buffer
  • PBS
  • Preservative
  • No preservatives
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • The antibody H17E2 has been reported in applications of Immunohistochemistry, Enzyme-linked Immunosorbent Assay, Western Blot, Immunocytochemistry, Inhibition. It's recommended that the optimal antibody concentration, dilution, incubition time etc. be carefully titrated in specific assays.

Target

  • Alternative Names
  • Alkaline Phosphatase, Placental; Alkaline Phosphatase Regan Isozyme; Placental Alkaline Phosphatase 1; EC 3.1.3.1; PLAP-1; PLAP; Alkaline Phosphatase, Placental (Regan Isozyme); H17E2; H17EZ

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "Clone H17E2"

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Single-domain Antibody

CAT Product Name Application Type
NAB-146-sdAb Recombinant Anti-Human ALPP VHH Single Domain Antibody FUNC, FA, IP, ChiP Llama VHH

Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-0157 Hi-Affi™ Rabbit Anti-ALPP Recombinant Antibody (clone DS157AB) IHC-P, WB Rabbit IgG

scFv Fragment Antibody

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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