Mouse Anti-AMACR Recombinant Antibody (clone 4A12) (CAT#: HPAB-0570-YJ)
This product is a recombinant mouse antibody that recognizes (both natural and recombinant) human AMACR. It can be used for scientific research or clinical expression of P504S immunohistochemical detection.
Specific Inquiry
IHC
Figure 1 Mouse Anti-AMACR Recombinant Antibody (clone 4A12) in IHC
Immunohistochemical analysis of paraffin-embedded Rat-kidney tissue. 1.AMACR Monoclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for Antibody retrieval(>98°C, 20min). 3, Secondary Antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary Antibody only.
IHC
Figure 2 Mouse Anti-AMACR Recombinant Antibody (clone 4A12) in IHC
IHC staining of mouse prostate adenocarcinoma tissue, diluted at 1:200.
IF
Figure 3 Mouse Anti-AMACR Recombinant Antibody (clone 4A12) in IF
Immunofluorescence analysis of Mouse-kidney tissue. 1.AMACR Monoclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary Antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI.Picture C: merge of A+B
IF
Figure 4 Mouse Anti-AMACR Recombinant Antibody (clone 4A12) in IF
Immunofluorescence analysis of Rat-brain tissue. 1.AMACR Monoclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary Antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C:merge of A+B
WB
Figure 5 Mouse Anti-AMACR Recombinant Antibody (clone 4A12) in WB
Western blot analysis of 1) HepG2, 2) Mouse Kidney, diluted at 1:1000.
Specifications
- Immunogen
- Recombinant human P504S expressed in E. coli
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human AMACR
- Species Reactivity
- Human
- Clone
- 4A12
- Applications
- ELISA, IHC
- Related Disease
- Cancer
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- RM; RACE; CBAS4; P504S; AMACRD; alpha-methylacyl-CoA racemase; 2-methylacyl-CoA racemase
- Gene ID
- 23600
- UniProt ID
- Q9UHK6
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IgG Antibody Products
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1622z | Mouse Anti-AMACR Recombinant Antibody (clone 7B6) | WB, ELISA, IF, IHC | Mouse IgG2b |
| ZG-0283F | Mouse Anti-AMACR Recombinant Antibody (ZG-0283F) | WB, IHC, IF, ELISA | Mouse IgG |
| ZG-0284F | Mouse Anti-AMACR Recombinant Antibody (ZG-0284F) | WB, ICC | Mouse IgG |
| VS3-WK1193 | Mouse Anti-AMACR Recombinant Antibody (VS3-WK1193) | WB, IHC | Mouse IgG1 |
| VS3-WK1194 | Mouse Anti-AMACR Recombinant Antibody (clone PT1743) | IHC-P, WB | Mouse IgG1, κ |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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