Mouse Anti-ANPEP Recombinant Antibody (clone 22A5) (CAT#: VS3-CJ662)
This product is a mouse antibody that recognizes human ANPEP (CD13).
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line BJ shows localization to plasma membrane.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4625/1612_A1_3_selected.jpg
(Immunofluorescent staining of human cell line BJ shows localization to plasma membrane.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4625/1612_A1_3_selected.jpg
Figure 2 Colon
(Enterocytes - Microvilli
Staining: High
Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2417/6169_A_9_3.jpg
(Enterocytes - Microvilli
Staining: High
Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2417/6169_A_9_3.jpg
Figure 3 Liver
(Cholangiocytes
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Hepatocytes
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2417/6169_A_9_4.jpg
(Cholangiocytes
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Hepatocytes
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2417/6169_A_9_4.jpg
Figure 4 Gallbladder
(Glandular cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2417/6169_A_5_4.jpg
(Glandular cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2417/6169_A_5_4.jpg
Figure 5 Kidney
(Proximal tubules (cell body)
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Proximal tubules (microvilli)
Staining: High
Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2417/6169_A_9_5.jpg
(Proximal tubules (cell body)
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Proximal tubules (microvilli)
Staining: High
Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2417/6169_A_9_5.jpg
Figure 6 RNA cell line category: Cell line enhanced (ASC diff, ASC TERT1, BJ, BJ hTERT+, fHDF/TERT166, HMC-1, HSkMC, HUVEC TERT2, TIME, U-2197)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000166825-ANPEP
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000166825-ANPEP
Specifications
- Immunogen
- Native purified human CD13
- Host Species
- Mouse
- Type
- Mouse IgG2a
- Specificity
- Human ANPEP (CD13)
- Species Reactivity
- Human
- Clone
- 22A5
- Applications
- IF, FC
- Conjugate
- Unconjugated
Product Property
- Purification
- Affinity chromatography
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Buffer
- PBS, pH 7.3.
- Preservative
- 0.02% Sodium Azide
- Storage
- Store at 4°C for one year. Do not freeze.
- Shipping
- Shipped at 4°C
Applications
- Application Notes
- This antibody has been tested for use in Immunofluorescence, Flow Cytometry.
Target
- Alternative Names
- APN; AP-M; AP-N; CD13; LAP1; P150; PEPN; hAPN; GP150
- Gene ID
- 290
- UniProt ID
- P15144
- Sequence Similarities
- Belongs to the peptidase M1 family.
- Cellular Localization
- Cell membrane, Membrane
- Post Translation Modifications
- Sulfated.
N- and O-glycosylated.
May undergo proteolysis and give rise to a soluble form.
- Protein Refseq
- NP_001141.2
- Function
- Broad specificity aminopeptidase which plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. Also involved in the processing of various peptides including peptide hormones, such as angiotensin III and IV, neuropeptides, and chemokines. May also be involved the cleavage of peptides bound to major histocompatibility complex class II molecules of antigen presenting cells. May have a role in angiogenesis and promote cholesterol crystallization. May have a role in amino acid transport by acting as binding partner of amino acid transporter SLC6A19 and regulating its activity (By similarity).
Recommended Products
Secondary Antibody
Recommended Dilution Buffer
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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Non-Human Primate (NHP) Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-69-F(E) | Recombinant Anti-human ANPEP Antibody Fab Fragment | WB, IP, FuncS | Fab |
| MHH-69-F(E) | Recombinant Human Anti-human ANPEP Antibody Fab Fragment | WB, FC, FuncS | Fab |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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