CytoStream™ Rabbit Anti-ANXA2 Recombinant Antibody (VS13-YC52)
CAT#: VS13-YC52
The CytoStream™ Rabbit Anti-ANXA2 Recombinant Antibody is specific for human and mouse ANXA2. ANXA2 encodes an annexin family protein that facilitates osteoclast formation and bone resorption, linked to three pseudogenes, with multiple spliced variants manifesting in different isoforms. The anti-ANXA2 antibody was developed and selected by our CytoStream™ platform and the mAb is recommended for use in flow cytometry staining (1/50-1/200).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/46964/1865_G3_2_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/61798/138247_B_9_5.jpg
Normal Tissue
Figure 3 Lung
Alveolar cells type I Staining: Medium Intensity: Moderate Quantity:>75% Alveolar cells type I Endothelial cells Staining: Low Intensity: Weak Quantity:>75% Macrophages Staining: High Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/61798/138247_A_3_4.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/61798/138247_A_9_3.jpg
Normal Tissue
Figure 5 Liver
Cholangiocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/61798/138247_A_7_4.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/61798/138247_A_7_5.jpg
Normal Tissue
Figure 7 Lymph node
Non-germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/61798/138247_A_8_8.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000182718-ANXA2
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Specifications
- Immunogen
- Recombinant human Annexin A2, aa 26-75.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Mouse ANXA2
- Species Reactivity
- Human, Mouse
- Applications
- WB, IHC-P, ICC, IF, FC
- Conjugate
- NONE
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- TBS (pH 7.4) containing 40% Glycerol and 0.05% BSA.
- Preservative
- 0.05% Sodium azide
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- Western blot (WB): 1/500-1/2000
Immunohistochemistry-paraffin (IHC-P): 1/50-1/200
Immunocytochemistry (ICC): 1/100
Immunofluorescence (IF): 1/100-1/200
Flow cytometry (FC): 1/50-1/200
Target
- Alternative Names
- Annexin A2; Annexin II; Placental Anticoagulant Protein IV; Calpactin I Heavy Chain; Calpactin-1 Heavy Chain; Lipocortin II; Protein I; Annexin-2; ANX2L4; PAP-IV; CAL1H;
- Gene ID
- 302
- UniProt ID
- P07355
- Long Name
- Annexin A2
- Cellular Localization
- Basement membrane; Melanosome
- Function
- Signal transduction
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "ANXA2"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0622MZ | Mouse Anti-ANXA2 Recombinant Antibody (clone 1G7) | IF, WB | Mouse IgG1 |
| MRO-0099-CN | Rabbit Anti-ANXA2 Recombinant Antibody (clone JA42-30) | WB, IHC, IF, FC | Rabbit IgG |
| ZG-0185U | Mouse Anti-ANXA2 Recombinant Antibody (clone 1A1E1) | ELISA, WB, IHC | Mouse IgG1 |
| VS3-FY80 | Recombinant Rabbit Anti-ANXA2 Antibody (clone R09-5C1) | WB, ICC, IF | Rabbit IgG |
| VS4-WK32 | Rabbit Anti-ANXA2 Recombinant Antibody (clone 10H9) | ELISA, WB, IHC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0184 | Hi-Affi™ Rabbit Anti-ANXA2 Recombinant Antibody (clone DS184AB) | WB, IHC-P, IF, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY119 | Anti-ANXA2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY411 | Anti-Mouse ANXA2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY410 | Anti-Human ANXA2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY412 | Anti-Chicken ANXA2 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0625-FY2 | Mouse Anti-ANXA2 scFv-Fc Chimera (VS-0625-FY2) | FC, IP, ADCC, Cyt | Mouse IgM, scFv-Fc |
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