Rabbit Anti-ANXA4 Recombinant Antibody (clone R04-3H8) (CAT#: VS3-FY1970)
The R04-3H8 is a rabbit recombinant monoclonal antibody that has been developed to detect human ANXA4. It cross-reacts with mouse, rat, hamster. It is suitable for use in a variety of assays, including WB, IHC-F, IHC-P, ICC/IF. The R04-3H8 is recommended for dilution of target protein by western blot (dilution 1/500-1/1000), immunohistochemistry (dilution 1/50-1/100), immunofluorescence (dilution 1/50-1/200).
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ICC
Figure 1 Anti-ANXA4 Antibody (VS3-FY1970) in ICC.
Immunocytochemistry staining of Annexin 4 (green) in Hela using the anti-Annexin 4 antibody. Nuclear DNA was labeled with DAPI (shown in blue).
WB
Figure 2 Anti-ANXA4 Antibody (VS3-FY1970) in WB.
The Annexin4/ANXA4 expression in mouse kidney, mouse lung lysates were detected using the anti-Annexin4/ANXA4 by western blot assay.
Specifications
- Immunogen
- Recombinant human Annexin-4/ANXA4 protein
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse, Rat, Hamster ANXA4
- Species Reactivity
- Human, Mouse, Rat, Hamster
- Clone
- R04-3H8
- Applications
- Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunofluorescence
- MW
- The calculated weight of the target protein is approximately 36 kDa, while the observed molecular weight stands at 36 kDa.
- Related Disease
- Chronic Gonococcal Salpingitis and Ovarian Clear Cell Adenocarcinoma
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Immunofluorescence: 1/50-1/200
Target
- Alternative Names
- Annexin A4; Carbohydrate-Binding Protein P33/P41; Placental Anticoagulant Protein II; 35-Beta Calcimedin; Chromobindin-4; Lipocortin IV; Endonexin I; Protein II; Annexin-4; PAP-II; P32.5
- Gene ID
- 307
- UniProt ID
- P09525
- Sequence Similarities
- Belongs to the annexin family.
- Cellular Localization
- Cytoplasmic vesicle, Membrane
- Post Translation Modifications
- Ubiquitination at Lys293 (NX_P09525 [NX_P09525-1])
Modification sites at PhosphoSitePlus (P09525)
Glycosylation from GlyGen (P09525) 1 site, 1 O-linked glycan (1 site)
- Protein Refseq
- XP_016859432.1; XP_024308603.1; XP_047300036.1; XP_047300037.1; XP_047300038.1
- Function
- Calcium/phospholipid-binding protein which promotes membrane fusion and is involved in exocytosis.
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IgG Antibody Products
Rabbit Monoclonal Antibody Products
Antibody Magnetic Beads
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1621z | Mouse Anti-ANXA4 Recombinant Antibody (clone 2A5) | WB, ELISA, IHC | Mouse IgG2a, κ |
| MOB-0623MZ | Mouse Anti-ANXA4 Recombinant Antibody (clone 2E4) | ELISA, WB | Mouse IgG2a |
| MRO-0100-CN | Rabbit Anti-ANXA4 Recombinant Antibody (clone JG37-83) | WB, IF, IHC, FC | Rabbit IgG |
| FAMAB-1012CQ | Mouse Anti-ANXA4 Recombinant Antibody (clone SAIC-14C-10F12) | WB, Immuno-MRM, ELISA | Mouse IgG1 |
| VS-0923-FY16 | Recombinant Mouse Anti-ANXA4 Antibody (VS-0923-FY16) | ELISA, WB | Mouse IgG1 |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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