Rabbit Anti-ANXA4 Recombinant Antibody (clone R04-3H8)
CAT#: VS3-FY1970
The R04-3H8 is a rabbit recombinant monoclonal antibody that has been developed to detect human ANXA4. It cross-reacts with mouse, rat, hamster. It is suitable for use in a variety of assays, including WB, IHC-F, IHC-P, ICC/IF. The R04-3H8 is recommended for dilution of target protein by western blot (dilution 1/500-1/1000), immunohistochemistry (dilution 1/50-1/100), immunofluorescence (dilution 1/50-1/200).
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7393/if_selected.jpg
Normal Tissue
Figure 2 Liver
Cholangiocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/17560/40847_A_8_4.jpg
Normal Tissue
Figure 3 Gallbladder
Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/17560/40847_A_6_4.jpg
Normal Tissue
Figure 4 Kidney
Bowman's capsule Staining: High Intensity: Strong Quantity:>75% Collecting ducts Staining: High Intensity: Strong Quantity: 75%-25% Distal tubules Staining: High Intensity: Strong Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/17560/40847_A_7_5.jpg
Normal Tissue
Figure 5 Colon
Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/17560/40847_A_9_3.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced (EFO-21, Hep G2, RPTEC TERT1)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000196975-ANXA4
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ICC
Figure 1 Anti-ANXA4 Antibody (VS3-FY1970) in ICC.
Immunocytochemistry staining of Annexin 4 (green) in Hela using the anti-Annexin 4 antibody. Nuclear DNA was labeled with DAPI (shown in blue).
WB
Figure 2 Anti-ANXA4 Antibody (VS3-FY1970) in WB.
The Annexin4/ANXA4 expression in mouse kidney, mouse lung lysates were detected using the anti-Annexin4/ANXA4 by western blot assay.
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Specifications
- Immunogen
- Recombinant human Annexin-4/ANXA4 protein
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse, Rat, Hamster ANXA4
- Species Reactivity
- Human, Mouse, Rat, Hamster
- Clone
- R04-3H8
- Applications
- Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunofluorescence
- MW
- The calculated weight of the target protein is approximately 36 kDa, while the observed molecular weight stands at 36 kDa.
- Related Disease
- Chronic Gonococcal Salpingitis and Ovarian Clear Cell Adenocarcinoma
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Immunofluorescence: 1/50-1/200
Target
- Alternative Names
- Annexin A4; Carbohydrate-Binding Protein P33/P41; Placental Anticoagulant Protein II; 35-Beta Calcimedin; Chromobindin-4; Lipocortin IV; Endonexin I; Protein II; Annexin-4; PAP-II; P32.5
- Gene ID
- 307
- UniProt ID
- P09525
- Sequence Similarities
- Belongs to the annexin family.
- Cellular Localization
- Cytoplasmic vesicle, Membrane
- Post Translation Modifications
- Ubiquitination at Lys293 (NX_P09525 [NX_P09525-1])
Modification sites at PhosphoSitePlus (P09525)
Glycosylation from GlyGen (P09525) 1 site, 1 O-linked glycan (1 site)
- Protein Refseq
- XP_016859432.1; XP_024308603.1; XP_047300036.1; XP_047300037.1; XP_047300038.1
- Function
- Calcium/phospholipid-binding protein which promotes membrane fusion and is involved in exocytosis.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "ANXA4"
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1621z | Mouse Anti-ANXA4 Recombinant Antibody (clone 2A5) | WB, ELISA, IHC | Mouse IgG2a, κ |
| MOB-0623MZ | Mouse Anti-ANXA4 Recombinant Antibody (clone 2E4) | ELISA, WB | Mouse IgG2a |
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| VS-0923-FY16 | Recombinant Mouse Anti-ANXA4 Antibody (VS-0923-FY16) | ELISA, WB | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0185 | Hi-Affi™ Rabbit Anti-ANXA4 Recombinant Antibody (clone DS185AB) | WB, ICC, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC1397 | AbPlus™ Anti-ANXA4 Magnetic Beads (VS-0724-YC1397) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY415 | Anti-ANXA4 Immunohistochemistry Kit | IHC |
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