Rabbit Anti-ANXA7 Recombinant Antibody (clone R07-3F6)
CAT#: VS3-FY1649
Clone R07-3F6 is a rabbit recombinant antibody that has been developed to detect human ANXA7 protein, a calculated weight of 53 kDa. It cross-reacts with rat.
The following recommendations serve as a starting point for assay optimization. The actual working concentration may vary and should be determined by the user. The R07-3F6 is recommended for detection of target protein by western blotting (dilution range 1:500-1:1000), immunohistochemistry (dilution range 1:50-1:100), immunoprecipitation (dilution range 1:20).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/64290/1200_H2_1_selected.jpg
Normal Tissue
Figure 2 IHC staining of human pancreas
Immunohistochemical staining of human pancreas shows strong cytoplasmic and membranous positivity in islet cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4312/ihc_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuronal cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4312/13119_B_8_5.jpg
Normal Tissue
Figure 4 Thyroid gland
Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4312/13119_B_2_5.jpg
Normal Tissue
Figure 5 Liver
Hepatocytes Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4312/13119_A_9_4.jpg
Normal Tissue
Figure 6 Kidney
Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4312/13119_A_9_5.jpg
Normal Tissue
Figure 7 Testis
Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4312/13119_A_4_6.jpg
Normal Tissue
Figure 8 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Nuclear Non-germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4312/13119_A_8_8.jpg
RNA Expression
Figure 9 RNA cell line category: Cell line enhanced (PC-3)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000138279-ANXA7
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Specifications
- Immunogen
- Recombinant human annexin VII protein
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Rat ANXA7
- Species Reactivity
- Human, Rat
- Clone
- R07-3F6
- Applications
- Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation
- MW
- The calculated weight of the target protein is approximately 53 kDa, while the observed molecular weight stands at 51,47 kDa.
- Related Disease
- Developmental And Epileptic Encephalopathy 33 and Gastric Tubular Adenocarcinoma
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Immunoprecipitation: 1/20
Target
- Alternative Names
- Annexin A7; Annexin VII; Annexin-7; SYNEXIN; ANX7; SNX
- Gene ID
- 310
- UniProt ID
- P20073
- Sequence Similarities
- Belongs to the annexin family.
- Protein Refseq
- NP_001147.1; NP_001307808.1; NP_001307809.1; NP_004025.1
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "ANXA7"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0040MZ | Chicken Anti-ANXA7 (ab1) Polyclonal IgY | WB | Chicken antibody |
| BRD-0041MZ | Chicken Anti-ANXA7 (ab2) Polyclonal IgY | WB | Chicken antibody |
| BRD-1024MZ | Chicken Anti-Annexin A7 Polyclonal IgY | WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY122 | Anti-ANXA7 Immunohistochemistry Kit | IHC | |
| VS-0525-XY420 | Anti-Mouse ANXA7 Immunohistochemistry Kit | IHC | |
| VS-0525-XY419 | Anti-Human ANXA7 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC53 | Rabbit Anti-ANXA7 Recombinant Antibody (VS13-YC53) | IHC-P | Rabbit IgG |
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