Mouse Anti-AOC3 Recombinant Antibody (clone TK8-14) (CAT#: FAMAB-1005CQ)
This product is a recombinant mouse antibody that recognizes AOC3. The antibody was purified by affinity chromatography. It can be used in IP, WB, ELISA, FC, IHC.
Specific Inquiry
IF
Figure 1 Confocal immunofluorescence of pericryptal MFs in a normal colon cryostat section.
AOC3 (TK8-14) is shown in green and DAPI in blue in all cases where not otherwise mentioned. (Magnification: 100×.) The red arrow identifies the MFs.
Hsia, L. T., Ashley, N., Ouaret, D., Wang, L. M., Wilding, J., & Bodmer, W. F. (2016). Myofibroblasts are distinguished from activated skin fibroblasts by the expression of AOC3 and other associated markers. Proceedings of the National Academy of Sciences, 113(15), E2162-E2171.
IF
Figure 2 AOC3 is expressed in primary MFs but not in fibroblasts.
Immunofluorescent staining of CCD 18CO cells and foreskin fibroblast cells with AOC3 mAb (TK8-14). (Magnification: 20×.)
Hsia, L. T., Ashley, N., Ouaret, D., Wang, L. M., Wilding, J., & Bodmer, W. F. (2016). Myofibroblasts are distinguished from activated skin fibroblasts by the expression of AOC3 and other associated markers. Proceedings of the National Academy of Sciences, 113(15), E2162-E2171.
WB
Figure 3 AOC3 is expressed in primary MFs but not in fibroblasts.
Western blot using AOC3 mAb (TK8-14) detected a 150-kDa protein in CCD 18CO, myo2020, myo1998, and myo6544 cells but not in foreskin fibroblasts (F. Fibro) or skin fibroblasts (Skin Fibro).
Hsia, L. T., Ashley, N., Ouaret, D., Wang, L. M., Wilding, J., & Bodmer, W. F. (2016). Myofibroblasts are distinguished from activated skin fibroblasts by the expression of AOC3 and other associated markers. Proceedings of the National Academy of Sciences, 113(15), E2162-E2171.
FC
Figure 4 AOC3 is expressed in primary MFs but not in fibroblasts.
Isotype control is shown in red, and anti-AOC3 (TK8-14) is shown in green.
Hsia, L. T., Ashley, N., Ouaret, D., Wang, L. M., Wilding, J., & Bodmer, W. F. (2016). Myofibroblasts are distinguished from activated skin fibroblasts by the expression of AOC3 and other associated markers. Proceedings of the National Academy of Sciences, 113(15), E2162-E2171.
FC
Figure 5 FACS isolation of MFs.
FACS isolation of MFs from fresh tissue. Cells expressing AOC3 (mAb TK8-14) were gated using the Alexa-488 fluorescent signals (y axis) with a cutoff based on unstained samples. The x axis is forward scatter.
Hsia, L. T., Ashley, N., Ouaret, D., Wang, L. M., Wilding, J., & Bodmer, W. F. (2016). Myofibroblasts are distinguished from activated skin fibroblasts by the expression of AOC3 and other associated markers. Proceedings of the National Academy of Sciences, 113(15), E2162-E2171.
Specifications
- Immunogen
- Affinity purified VAP-1
- Host Species
- Mouse
- Type
- Mouse IgG2a
- Specificity
- Human Vascular Adhesion Protein-1
- Species Reactivity
- Human
- Clone
- TK8-14
- Applications
- IF, WB, FC
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been reported for use in Immunofluorescence, Western Blot and Flow Cytometry.
Target
- Alternative Names
- Amine Oxidase, Copper Containing 3; Semicarbazide-Sensitive Amine Oxidase; Vascular Adhesion Protein 1; Copper Amine Oxidase; EC 1.4.3.21; VAP-1; SSAO
- Gene ID
- 8639
- UniProt ID
- Q16853
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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Antibody Magnetic Beads
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-261 | Anti-Human AOC3 Recombinant Antibody (Vapaliximab) | WB, FC, IP, ELISA, Neut, FuncS, IF | IgG2 |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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