Rabbit Anti-APBB1 Recombinant Antibody (clone R02-0C4) (CAT#: VS3-FY2002)

• Immunogen
• Synthetic peptide of human FE65

• Host Species
• Rabbit

• Type
• Rabbit IgG

• Specificity
• Human APBB1

• Species Reactivity
• Human

• Clone
• R02-0C4

• Applications
• Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunofluorescence

• MW
• The calculated weight of the target protein is approximately 77 kDa, while the observed molecular weight stands at 100 kDa.

• Related Disease
• Alzheimer's diseases

• Purification
• Affinity Purified

• Format
• Liquid

• Concentration
• Please refer to the vial label for the specific concentration.

• Buffer
• 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.05% BSA

• Preservative
• 0.01% Sodium azide

• Storage
• Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

• Application Notes
• Western Blot: 1/500-1/1000
  Immunohistochemistry: 1/50-1/100
  Immunofluorescence: 1/50-1/200

• Alternative Names
• Amyloid Beta Precursor Protein Binding Family B Member 1; Amyloid Beta (A4) Precursor Protein-Binding, Family B, Member 1 (Fe65); FE65; RIR; Amyloid Beta A4 Precursor Protein-Binding Family B Member 1; Amyloid-Beta A4 Precursor Protein-Binding Family B Member 1

• Gene ID
• 322

• UniProt ID
• O00213

• Cellular Localization
• Cell membrane, Cell projection, Cytoplasm, Membrane, Nucleus

• Post Translation Modifications
• Phosphorylation at Ser-610 by SGK1 promotes its localization to the nucleus ().
  Phosphorylated following nuclear translocation (PubMed:15031292, 18922798).
  Phosphorylation at Tyr-547 by ABL1 enhances transcriptional activation activity and reduces the affinity for RASD1/DEXRAS1 (PubMed:15031292, 18922798).
  Acetylation at Lys-204 and Lys-701 by KAT5 promotes its transcription activator activity.
  Polyubiquitination by RNF157 leads to degradation by the proteasome (PubMed:25342469).
  Ubiquitination at Lys48 (NX_O00213 [NX_O00213-1])
  Modification sites at PhosphoSitePlus (O00213)
  Glycosylation from GlyGen (O00213) 1 site, 1 O-linked glycan (1 site)

• Protein Refseq
• NP_001155.1; NP_001244248.1; NP_001244249.1; NP_001244250.1; NP_001244252.1

• Function
• May act by specifically recognizing and binding histone H2AX phosphorylated on 'Tyr-142' (H2AXY142ph) at double-strand breaks (DSBs), recruiting other pro-apoptosis factors such as MAPK8/JNK1.
  Required for histone H4 acetylation at double-strand breaks (DSBs).
  Its ability to specifically bind modified histones and chromatin modifying enzymes such as KAT5/TIP60, probably explains its transcription activation activity.
  Functions in association with TSHZ3, SET and HDAC factors as a transcriptional repressor, that inhibits the expression of CASP4.

Isotype Control

• MOB-208CQ
• Rabbit IgG Isotype Control Antibody

Secondary Antibody

• 2ND-054CQ
• Goat Anti-Rabbit IgG Antibody, R-PE

• 2ND-059CQ
• Goat Anti-Rabbit IgG (H&L) Antibody

• 2ND-055CQ
• Chicken Anti-Rabbit IgG (H&L) Antibody

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