Mouse Anti-APOB Recombinant Antibody (clone MabB9) (CAT#: FAMAB-0014CQ)
This product is a recombinant mouse antibody clone MabB9, which specifically binds to APOB.
Specific Inquiry
ELISA
Figure 1 Mouse Anti-APOB Antibody (clone MabB9) in ELISA.
Detection of LDL with phage-displayed antibodies (ScFvB9 and ScFvB9-6) by ELISA. Phage-displayed antibodies were produced from E. coli TG1 with pCAN-ScFvB9 and pCAN-ScFvB9-6. LDL, BSA, and albumin were used as antigen. Each antigen-bound phage-displayed antibody was detected by ELISA using HRP/Anti-M13 conjugated.
Yang, E. J. , Kwak, J. W. , & Chang, H. C. . (2001). Expression and detection of scfvb9 and its mutant in recombinant phage antibody system. Hybrid Hybridomics, 20(5-6), 369-375.
ELISA
Figure 2 Mouse Anti-APOB Antibody (clone MabB9) in ELISA.
Detection of LDL using soluble antibodies (ScFvB9 and ScFvB9-6). Soluble antibodies were produced from E. coli HB2151. Soluble antibodies were detected by ELISA using HRP/Anti-E Tag conjugate; primary antibodies were soluble antibodies ScFvB9 and ScFvB9-6, and secondary antibody was HRP/Anti-E Tag conjugate, which was diluted 1: 10,000 in PBS.
Yang, E. J. , Kwak, J. W. , & Chang, H. C. . (2001). Expression and detection of scfvb9 and its mutant in recombinant phage antibody system. Hybrid Hybridomics, 20(5-6), 369-375.
ELISA
Figure 3 Mouse Anti-APOB Antibody (clone MabB9) in ELISA.
SDS-PAGE and Western blotting of soluble antibodies. Samples were separated by 15% SDS-PAGE and stained with Coomassie brilliant blue (A), and then transferred onto nitrocellulose membrane for Western blot analysis (B). Lanes: 1, ScFvB9 soluble antibody; 2, ScFvB9-6 soluble antibody; 3, proteins in the supernatant of the E. coli HB2151 culture.
Yang, E. J. , Kwak, J. W. , & Chang, H. C. . (2001). Expression and detection of scfvb9 and its mutant in recombinant phage antibody system. Hybrid Hybridomics, 20(5-6), 369-375.
Specifications
- Immunogen
- Human Apolipoprotein B-100
- Host Species
- Mouse
- Type
- Mouse IgG2b, κ
- Specificity
- Human plasma apolipoprotein B-100
- Species Reactivity
- Human
- Clone
- MabB9
- Applications
- ELISA
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- Apolipoprotein B; Apolipoprotein B (Including Ag(X) Antigen); Apolipoprotein B48; Apo B-100; ApoB-100; ApoB-48; LDLCQ4; FLDB
- Gene ID
- 338
- UniProt ID
- P04114
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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See other products for "Clone MabB9"
See other products for "APOB"
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1364z | Mouse Anti-APOB Recombinant Antibody (clone 21F3) | ELISA, FC, ICC, IF, WB | Mouse IgG1 |
| MOB-0666MZ | Mouse Anti-APOB Recombinant Antibody (clone 23H20) | FC | Mouse IgG1 |
| FAMAB-0013CQ | Mouse Anti-APOB Recombinant Antibody (clone MabB23) | ELISA | Mouse IgG2b, λ |
| VS3-XY84 | Mouse Anti-APOB Recombinant Antibody (clone 6G6) | ELISA, WB, ICC, FC | Mouse IgG1 |
Single-domain Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NABG-015 | Recombinant Anti-Human Apob VHH Single Domain Antibody | CA, IP, IF, FuncS | Llama VHH |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0207 | Hi-Affi™ Rabbit Anti-APOB Recombinant Antibody (clone DS207AB) | WB, ICC | Rabbit IgG |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0013CQ-F(E) | Mouse Anti-APOB Recombinant Antibody (clone MabB23); Fab Fragment | ELISA | Mouse Fab |
| FAMAB-0014CQ-F(E) | Mouse Anti-APOB Recombinant Antibody (clone MabB9); Fab Fragment | ELISA | Mouse Fab |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0013CQ-S(P) | Mouse Anti-APOB Recombinant Antibody (clone MabB23); scFv Fragment | ELISA | Mouse scFv |
| FAMAB-0014CQ-S(P) | Mouse Anti-APOB Recombinant Antibody (clone MabB9); scFv Fragment | ELISA | Mouse scFv |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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