Rabbit Anti-APOB Recombinant Antibody (clone R02-6G3) (CAT#: VS3-XY3262)
This is a recombinant rabbit antibody targeting the human Apolipoprotein B (APOB), which functions as a recognition signal for the cellular binding and internalization of LDL particles by the apoB/E receptor. It has been determined using Western Blot, Immunoprecipitation and Flow cytometry.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line Hep G2
(Immunofluorescent staining of human cell line Hep G2 shows localization to cytosol & vesicles.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/49793/762_C6_1_selected.jpg
(Immunofluorescent staining of human cell line Hep G2 shows localization to cytosol & vesicles.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/49793/762_C6_1_selected.jpg
Figure 2 IHC staining of human duodenum
(Immunohistochemical staining of human duodenum shows distinct positivity in plasma.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/49793/ihc_selected.jpg
(Immunohistochemical staining of human duodenum shows distinct positivity in plasma.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/49793/ihc_selected.jpg
Figure 3 Duodenum
(Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/16070/35958_A_8_2.jpg
(Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/16070/35958_A_8_2.jpg
Figure 4 Colon
(Glandular cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/16070/35958_A_8_3.jpg
(Glandular cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/16070/35958_A_8_3.jpg
Figure 5 RNA cell line category: Cell line enriched (Hep G2)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000084674-APOB
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000084674-APOB
Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human APOB
- Species Reactivity
- Human
- Clone
- R02-6G3
- Applications
- WB, IP, FC
- Related Disease
- Hypobetalipoproteinemia, Familial, 1; Hypercholesterolemia, Familial, 2
Product Property
- Clonality
- Monoclonal
- Aggregation
- < 5%
- Format
- Liquid
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% glycerol
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IP: 1/20
FC: 1/50-1/100
Target
- Alternative Names
- FLDB; FCHL2; LDLCQ4; apoB-48; apoB-100
- Gene ID
- 338
- UniProt ID
- P04114
- Long Name
- Apolipoprotein B
- Cellular Localization
- Cytoplasm, Lipid droplet
- Post Translation Modifications
- Palmitoylated; structural requirement for proper assembly of the hydrophobic core of the lipoprotein particle.
- Protein Refseq
- NP_000375.3
- Function
- Apolipoprotein B is a major protein constituent of chylomicrons (apo B-48), LDL (apo B-100) and VLDL (apo B-100). Apo B-100 functions as a recognition signal for the cellular binding and internalization of LDL particles by the apoB/E receptor.
Recommended Products
Isotype Control
Recommended Dilution Buffer
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "APOB"
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1364z | Mouse Anti-APOB Recombinant Antibody (clone 21F3) | ELISA, FC, ICC, IF, WB | Mouse IgG1 |
| MOB-0666MZ | Mouse Anti-APOB Recombinant Antibody (clone 23H20) | FC | Mouse IgG1 |
| FAMAB-0013CQ | Mouse Anti-APOB Recombinant Antibody (clone MabB23) | ELISA | Mouse IgG2b, λ |
| FAMAB-0014CQ | Mouse Anti-APOB Recombinant Antibody (clone MabB9) | ELISA | Mouse IgG2b, κ |
| VS3-XY84 | Mouse Anti-APOB Recombinant Antibody (clone 6G6) | ELISA, WB, ICC, FC | Mouse IgG1 |
Single-domain Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NABG-015 | Recombinant Anti-Human Apob VHH Single Domain Antibody | CA, IP, IF, FuncS | Llama VHH |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0207 | Hi-Affi™ Rabbit Anti-APOB Recombinant Antibody (clone DS207AB) | WB, ICC | Rabbit IgG |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0013CQ-F(E) | Mouse Anti-APOB Recombinant Antibody (clone MabB23); Fab Fragment | ELISA | Mouse Fab |
| FAMAB-0014CQ-F(E) | Mouse Anti-APOB Recombinant Antibody (clone MabB9); Fab Fragment | ELISA | Mouse Fab |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0013CQ-S(P) | Mouse Anti-APOB Recombinant Antibody (clone MabB23); scFv Fragment | ELISA | Mouse scFv |
| FAMAB-0014CQ-S(P) | Mouse Anti-APOB Recombinant Antibody (clone MabB9); scFv Fragment | ELISA | Mouse scFv |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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