Rabbit Anti-APOE Recombinant Antibody (VS3-CJ300)
CAT#: VS3-CJ300
This product is a rabbit antibody that recognizes APOE.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to vesicles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/65539/1270_D3_2_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Neuronal cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous nuclear Neuropil Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/65539/154001_B_9_5.jpg
Normal Tissue
Figure 3 High expression in liver
Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/65539/154001_A_7_4.jpg
Normal Tissue
Figure 4 Testis
Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/65539/154001_A_6_6.jpg
RNA Expression
Figure 5 RNA cell line category: Cell line enhanced (ASC diff, CACO-2, Hep G2, HSkMC, SK-MEL-30)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000130203-APOE
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Specifications
- Immunogen
- Recombinant protein
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Mouse APOE
- Species Reactivity
- Human, Mouse
- Applications
- WB, ICC, IF, IHC, IP
- Conjugate
- Unconjugated
Product Property
- Purification
- Protein A affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- 40% Glycerol, 1% BSA, TBS, pH7.4.
- Preservative
- 0.05% sodium azide
- Storage
- Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- This antibody has been tested for use in Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation.
Target
- Alternative Names
- AD2; APO-E; ApoE4; LDLCQ5; LPG
- Gene ID
- 348
- UniProt ID
- P02649
- Sequence Similarities
- Belongs to the apolipoprotein A1/A4/E family.
- Cellular Localization
- Chylomicron, Endosome, Extracellular matrix, HDL, Secreted, VLDL
- Post Translation Modifications
- APOE exists as multiple glycosylated and sialylated glycoforms within cells and in plasma (PubMed:29516132).
The extent of glycosylation and sialylation are tissue and context specific (PubMed:29516132).
Plasma APOE undergoes desialylation and is less glycosylated and sialylated than the cellular form (PubMed:2498325, PubMed:19838169, PubMed:20511397, PubMed:23234360).
Glycosylation is not required for proper expression and secretion (PubMed:2498325).
O-glycosylated with core 1 or possibly core 8 glycans. Thr-307 and Ser-314 are minor glycosylation sites compared to Ser-308 (PubMed:19838169, PubMed:23234360).
Glycated in plasma VLDL of normal subjects, and of hyperglycemic diabetic patients at a higher level (2-3 fold).
Phosphorylated by FAM20C in the extracellular medium.
Undergoes C-terminal proteolytic processing in neurons. C-terminally truncated APOE has a tendency to form neurotoxic intracellular neurofibrillary tangle-like inclusions in neurons.
- Protein Refseq
- NP_000032.1; NP_001289617.1; NP_001289618.1
- Function
- APOE is an apolipoprotein, a protein associating with lipid particles, that mainly functions in lipoprotein-mediated lipid transport between organs via the plasma and interstitial fluids (PubMed:6860692, PubMed:1911868, PubMed:14754908).
APOE is a core component of plasma lipoproteins and is involved in their production, conversion and clearance (PubMed:6860692, PubMed:2762297, PubMed:1911868, PubMed:1917954, PubMed:9395455, PubMed:14754908, PubMed:23620513).
Apoliproteins are amphipathic molecules that interact both with lipids of the lipoprotein particle core and the aqueous environment of the plasma (PubMed:6860692, PubMed:2762297, PubMed:9395455).
As such, APOE associates with chylomicrons, chylomicron remnants, very low density lipoproteins (VLDL) and intermediate density lipoproteins (IDL) but shows a preferential binding to high-density lipoproteins (HDL) (PubMed:6860692, PubMed:1911868).
It also binds a wide range of cellular receptors including the LDL receptor/LDLR, the LDL receptor-related proteins LRP1, LRP2 and LRP8 and the very low-density lipoprotein receptor/VLDLR that mediate the cellular uptake of the APOE-containing lipoprotein particles (PubMed:2762297, PubMed:1917954, PubMed:7768901, PubMed:8939961, PubMed:12950167, PubMed:20030366, PubMed:2063194, PubMed:8756331, PubMed:20303980, PubMed:1530612, PubMed:7635945).
Finally, APOE has also a heparin-binding activity and binds heparan-sulfate proteoglycans on the surface of cells, a property that supports the capture and the receptor-mediated uptake of APOE-containing lipoproteins by cells (PubMed:9395455, PubMed:9488694, PubMed:23676495, PubMed:7635945).
A main function of APOE is to mediate lipoprotein clearance through the uptake of chylomicrons, VLDLs, and HDLs by hepatocytes (PubMed:1911868, PubMed:1917954, PubMed:9395455, PubMed:23676495, PubMed:29516132).
APOE is also involved in the biosynthesis by the liver of VLDLs as well as their uptake by peripheral tissues ensuring the delivery of triglycerides and energy storage in muscle, heart and adipose tissues (PubMed:2762297, PubMed:29516132).
By participating in the lipoprotein-mediated distribution of lipids among tissues, APOE plays a critical role in plasma and tissues lipid homeostasis (PubMed:2762297, PubMed:1917954, PubMed:29516132).
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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| HPAB-N0315-YC-S(P) | Mouse Anti-APOE Recombinant Antibody; scFv Fragment (HPAB-N0315-YC-S(P)) | ELISA, WB | Mouse scFv |
| HPAB-N0316-YC-S(P) | Mouse Anti-APOE Recombinant Antibody; scFv Fragment (HPAB-N0316-YC-S(P)) | ELISA, WB | Mouse scFv |
| HPAB-N0317-YC-S(P) | Mouse Anti-APOE Recombinant Antibody; scFv Fragment (HPAB-N0317-YC-S(P)) | IP, ELISA, WB | Mouse scFv |
| HPAB-N0318-YC-S(P) | Mouse Anti-APOE Recombinant Antibody; scFv Fragment (HPAB-N0318-YC-S(P)) | ELISA, WB | Mouse scFv |
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| CAT | Product Name | Application | Type |
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| HPAB-N0315-YC-F(E) | Mouse Anti-APOE Recombinant Antibody; Fab Fragment (HPAB-N0315-YC-F(E)) | ELISA, WB | Mouse Fab |
| HPAB-N0316-YC-F(E) | Mouse Anti-APOE Recombinant Antibody; Fab Fragment (HPAB-N0316-YC-F(E)) | ELISA, WB | Mouse Fab |
| HPAB-N0317-YC-F(E) | Mouse Anti-APOE Recombinant Antibody; Fab Fragment (HPAB-N0317-YC-F(E)) | IP, ELISA, WB | Mouse Fab |
| HPAB-N0318-YC-F(E) | Mouse Anti-APOE Recombinant Antibody; Fab Fragment (HPAB-N0318-YC-F(E)) | ELISA, WB | Mouse Fab |
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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