Mouse Anti-APP Recombinant Antibody (clone 2C1) (CAT#: HPAB-0988-FY)

This product is a recombinant antibody that recognizes APP. The monoclonal antibody 2C1 reacts with the epitope of APP.


Specific Inquiry
  • Size:
  • Conjugation:
  • Endotoxin:
  • Purity:
  • Fc Engineering:
  • Published Data
  • Gene Expression
  • Datasheet
  • MSDS
  • COA
ELISA

Figure 1 Comparing antibodies against Aβ3–7 in the ex vivo assay, IgG2a antibodies clear β amyloid plaque more efficiently that either the IgG1 or IgG2b isotypes.

Figure 1 Comparing antibodies against Aβ3–7 in the ex vivo assay, IgG2a antibodies clear β amyloid plaque more efficiently that either the IgG1 or IgG2b isotypes.

Murine primary microglial cells were cultured with unfixed cryostat sections of PDAPP mouse brain in the presence of antibodies of different isotypes directed against Aβ3–7. Irrelevant IgG1, IgG2a, and IgG2b antibodies were used as the respective isotype-matched negative controls. After 24 h of incubation, the total level of Aβ remaining in the cultures was measured by ELISA. The two anti-Aβ IgG2a antibodies reduced Aβ levels in the cultures (69% for 2C1 and 73% for 12B4; P < 0.001) more efficiently than the IgG2b isotype antibodies (48% for 12A11, P < 0.05, and 59% for 3A3, P < 0.001). The anti-Aβ IgG1 antibodies did not significantly reduce Aβ levels. Data were analyzed with ANOVA followed by a post hoc Dunnett's test.

Bard, F., Barbour, R., Cannon, C., Carretto, R., Fox, M., Games, D., ... & Khan, K. (2003). Epitope and isotype specificities of antibodies to β-amyloid peptide for protection against Alzheimer's disease-like neuropathology. Proceedings of the National Academy of Sciences, 100(4), 2023-2028.

FuncS

Figure 2 Anti-Aβ IgG2a antibodies reduced AD-like neuropathology more efficiently than other isotypes in vivo.

Figure 2 Anti-Aβ IgG2a antibodies reduced AD-like neuropathology more efficiently than other isotypes in vivo.

The percentage of frontal cortex occupied by neuritic dystrophy was determined by image analysis. The different groups within this experiment were analyzed in two sets by using the same PBS group as an internal standard (PBS-1 and PBS-2). PBS-1 was the control for the 6C6, 10D5, 12B4, and 12A11 groups, and PBS-2 was the control for the 2C1 and 3A3 groups. To compare the groups, values for individual animals are expressed as a percentage of the mean of their respective PBS control group (set at 100%). The bars represent median values, and the dashed horizontal line indicates the control level. Neuritic dystrophy was reduced significantly only by the IgG2a isotype antibodies (12B4, P < 0.05, and 2C1, P < 0.001; ANOVA followed by post hoc Dunnett's test).

Bard, F., Barbour, R., Cannon, C., Carretto, R., Fox, M., Games, D., ... & Khan, K. (2003). Epitope and isotype specificities of antibodies to β-amyloid peptide for protection against Alzheimer's disease-like neuropathology. Proceedings of the National Academy of Sciences, 100(4), 2023-2028.


Specifications

  • Host Species
  • Mouse
  • Type
  • Mouse IgG
  • Specificity
  • Human APP
  • Species Reactivity
  • Human
  • Clone
  • 2C1
  • Applications
  • ELISA, FuncS

Product Property

  • Purity
  • >95% as determined by SDS-PAGE and HPLC analysis
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Buffer
  • PBS
  • Preservative
  • No preservatives
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • The APP antibody has been reported in applications of Enzyme-linked Immunosorbent Assay, Functional Assay.

Target

  • Alternative Names
  • Amyloid Beta Precursor Protein; Amyloid Beta (A4) Precursor Protein; Alzheimer Disease Amyloid Protein; Cerebral Vascular Amyloid Peptide; Amyloid Precursor Protein; Peptidase Nexin-II; Protease Nexin-II; PreA4; PN-II; ABPP; APPI; CVAP; AD1; Beta-Amyloid Precursor Protein

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "Clone 2C1"

See other products for "APP"

Single-domain Antibody

CAT Product Name Application Type
TAB-0019CL-VHH Camelid Anti-APP Recombinant Antibody (TAB-0019CL-VHH) IHC, WB Camelid VHH
TAB-0020CL-VHH Anti-Human APP VHH Single Domain Antibody (MPL D7) ELISA, IHC Single domain antibody
TAB-0021CL-VHH Anti-Human APP VHH Single Domain Antibody (MPL C2) ELISA, In vivo, FuncS Single domain antibody
TAB-0022CL-VHH Anti-Human APP VHH Single Domain Antibody (MPL H3) ELISA, In vivo, FuncS Single domain antibody
TAB-0023CL-VHH Anti-Human APP VHH Single Domain Antibody (MPL H6) ELISA, In vivo, FuncS Single domain antibody

Chicken IgY Antibody

CAT Product Name Application Type
BRD-0685MZ Chicken Anti-APP Polyclonal IgY IHC Chicken antibody

ADCC Enhanced Antibody

CAT Product Name Application Type
AFC-TAB-225 Afuco™ Anti-APP ADCC Recombinant Antibody (Bapineuzumab), ADCC Enhanced FuncS, IF, Neut, ELISA, FC ADCC enhanced antibody
AFC-TAB-228 Afuco™ Anti-APP ADCC Recombinant Antibody (Solanezumab), ADCC Enhanced FuncS, IF, Neut, ELISA, FC ADCC enhanced antibody
AFC-TAB-229 Afuco™ Anti-APP ADCC Recombinant Antibody (Gantenerumab), ADCC Enhanced FuncS, IF, Neut, ELISA, FC, IP ADCC enhanced antibody
AFC-TAB-227 Afuco™ Anti-APP ADCC Recombinant Antibody (Ponezumab), ADCC Enhanced ELISA, IP, FC, FuncS, Neut ADCC enhanced antibody
AFC-TAB-226 Afuco™ Anti-APP ADCC Recombinant Antibody (Crenezumab), ADCC Enhanced IF, IP, Neut, FuncS, ELISA ADCC enhanced antibody

Customer Reviews and Q&As

Submit a review or a question
There are currently no Customer reviews or questions for HPAB-0988-FY. Click the button above to contact us or submit your feedback about this product.
View the frequently asked questions answered by Creative Biolabs Support.

For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

Send Inquiry

This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.

© 2024 Creative Biolabs.
  • 0
  • 0
Cart

    Go to compare