Rabbit Anti-APP Recombinant Antibody (clone 6F10)

Name: Rabbit Anti-APP Recombinant Antibody (clone 6F10)
SKU: ZG-0048U
Rating: 5 (1 reviews)

CAT#: ZG-0048U

The purified Rabbit Anti-APP Recombinant Antibody (clone 6F10) displayed high specificity against the APP protein of human, mouse and rat.

Tested Data Gene Expression

Figure 1 Rabbit Anti-APP Antibody (ZG-0048U) in WB

Positive WB detected in: Hela whole cell lysate, A375 whole cell lysate, HEK293 whole cell lysate, Rat Brain whole cell lysate, Mouse Brain whole cell lysate. All lanes: Amyloid beta A4 antibody at 1: 1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 87, 35, 77, 79, 79, 81, 83, 85, 86, 73, 85 kDa
Observed band size: 100 kDa

Figure 2 Rabbit Anti-APP Antibody (ZG-0048U) in IF

Immunofluorescence staining of Hela Cells with ZG-0048U at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).

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Subcellular Location Figure 1 IF staining of human cell line U-2 OS

Immunofluorescent staining of human cell line U-2 OS shows localization to the Golgi apparatus & vesicles.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1462/if_selected.jpg

Subcellular Location Figure 2 IF staining of human cell line A-431

Immunofluorescent staining of human cell line A-431 shows localization to the Golgi apparatus.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1462/60_E2_1_blue_red_green.jpg

Subcellular Location Figure 3 IF staining of human cell line U-251 MG

Immunofluorescent staining of human cell line U-251 MG shows localization to the Golgi apparatus.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1462/61_E2_1_blue_red_green.jpg

Normal Tissue Figure 4 Cerebral cortex

Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/157/2073_B_7_5.jpg

RNA Expression Figure 5 RNA cell line category: Low cell line specificity

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000142192-APP

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Specifications

Immunogen
A synthesized peptide derived from human Amyloid beta A4

Host Species
Rabbit

Type
Rabbit IgG

Specificity
Human APP

Species Reactivity
Human, Mouse, Rat

Clone
6F10

Applications
ELISA, WB, IF

Conjugate
Unconjugated

Related Disease
Cerebral Amyloid Angiopathy, App-Related and Alzheimer Disease

Product Property

Purification
Affinity purified

Concentration
See the COA

Buffer
PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Preservative
0.02% sodium azide

Storage
Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.

Applications

Application Notes
This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, western blot, Immunofluorescence. Specific conditions should be optimized by the end user.
WB:1:500-1:5000, IF:1:20-1:200

Target

Alternative Names
Amyloid-beta A4 protein; ABPP; APPI; APP; Alzheimer disease amyloid protein; Amyloid precursor protein; Amyloid-beta precursor protein; Cerebral vascular amyloid peptide; CVAP; PreA4; Protease nexin-II; PN-II; Cleaved into: N-APP; Soluble APP-alpha; S-APP-alpha; Soluble APP-beta;S-APP-beta; C99; Beta-secretase C-terminal fragment; Beta-CTF; Amyloid-beta protein 42; Abeta42; Beta-APP42; Amyloid-beta protein 40; Abeta40; Beta-APP40; C83; Alpha-secretase C-terminal fragment; Alpha-CTF; P3; 42; P3; 40; C80; Gamma-secretase C-terminal fragment 59; Amyloid intracellular domain 59; AICD-59; AID 59; Gamma-CTF 59; Gamma-secretase C-terminal fragment 57; Amyloid intracellular domain 57; AICD-57; AID 57; Gamma-CTF 57; Gamma-secretase C-terminal fragment 50; Amyloid intracellular domain 50; AICD-50; AID 50; Gamma-CTF 50; C31; APP; A4 AD1

Gene ID
351

UniProt ID
P05067

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Downloadable Resources

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - ZG-0048U.pdf

MSDS

MSDS - ZG-0048U.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

Lot Number:

Protocol & Troubleshooting

Immunofluorescence (IF)

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

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