Rabbit Anti-APP Recombinant Antibody (clone 6F10) (CAT#: ZG-0048U)

The purified Rabbit Anti-APP Recombinant Antibody (clone 6F10) displayed high specificity against the APP protein of human, mouse and rat.


Specific Inquiry
  • Size:
  • Conjugation:
  • Endotoxin:
  • Purity:
  • Fc Engineering:
  • Tested Data
  • Gene Expression
  • Datasheet
  • MSDS
  • COA
WB

Figure 1 Rabbit Anti-APP Antibody (ZG-0048U) in WB

Figure 1 Rabbit Anti-APP Antibody (ZG-0048U) in WB

Positive WB detected in: Hela whole cell lysate, A375 whole cell lysate, HEK293 whole cell lysate, Rat Brain whole cell lysate, Mouse Brain whole cell lysate. All lanes: Amyloid beta A4 antibody at 1: 1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 87, 35, 77, 79, 79, 81, 83, 85, 86, 73, 85 kDa
Observed band size: 100 kDa

IF

Figure 2 Rabbit Anti-APP Antibody (ZG-0048U) in IF

Figure 2 Rabbit Anti-APP Antibody (ZG-0048U) in IF

Immunofluorescence staining of Hela Cells with ZG-0048U at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).


Specifications

  • Immunogen
  • A synthesized peptide derived from human Amyloid beta A4
  • Host Species
  • Rabbit
  • Type
  • Rabbit IgG
  • Specificity
  • Human APP
  • Species Reactivity
  • Human, Mouse, Rat
  • Clone
  • 6F10
  • Applications
  • ELISA, WB, IF
  • Conjugate
  • Unconjugated
  • Related Disease
  • Cerebral Amyloid Angiopathy, App-Related and Alzheimer Disease

Product Property

  • Purification
  • Affinity purified
  • Concentration
  • See the COA
  • Buffer
  • PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Preservative
  • 0.02% sodium azide
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, western blot, Immunofluorescence. Specific conditions should be optimized by the end user.
    WB:1:500-1:5000, IF:1:20-1:200

Target

  • Alternative Names
  • Amyloid-beta A4 protein; ABPP; APPI; APP; Alzheimer disease amyloid protein; Amyloid precursor protein; Amyloid-beta precursor protein; Cerebral vascular amyloid peptide; CVAP; PreA4; Protease nexin-II; PN-II; Cleaved into: N-APP; Soluble APP-alpha; S-APP-alpha; Soluble APP-beta;S-APP-beta; C99; Beta-secretase C-terminal fragment; Beta-CTF; Amyloid-beta protein 42; Abeta42; Beta-APP42; Amyloid-beta protein 40; Abeta40; Beta-APP40; C83; Alpha-secretase C-terminal fragment; Alpha-CTF; P3; 42; P3; 40; C80; Gamma-secretase C-terminal fragment 59; Amyloid intracellular domain 59; AICD-59; AID 59; Gamma-CTF 59; Gamma-secretase C-terminal fragment 57; Amyloid intracellular domain 57; AICD-57; AID 57; Gamma-CTF 57; Gamma-secretase C-terminal fragment 50; Amyloid intracellular domain 50; AICD-50; AID 50; Gamma-CTF 50; C31; APP; A4 AD1

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

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ADCC Enhanced Antibody

CAT Product Name Application Type
AFC-TAB-225 Afuco™ Anti-APP ADCC Recombinant Antibody (Bapineuzumab), ADCC Enhanced FuncS, IF, Neut, ELISA, FC ADCC enhanced antibody
AFC-TAB-228 Afuco™ Anti-APP ADCC Recombinant Antibody (Solanezumab), ADCC Enhanced FuncS, IF, Neut, ELISA, FC ADCC enhanced antibody
AFC-TAB-229 Afuco™ Anti-APP ADCC Recombinant Antibody (Gantenerumab), ADCC Enhanced FuncS, IF, Neut, ELISA, FC, IP ADCC enhanced antibody
AFC-TAB-227 Afuco™ Anti-APP ADCC Recombinant Antibody (Ponezumab), ADCC Enhanced ELISA, IP, FC, FuncS, Neut ADCC enhanced antibody
AFC-TAB-226 Afuco™ Anti-APP ADCC Recombinant Antibody (Crenezumab), ADCC Enhanced IF, IP, Neut, FuncS, ELISA ADCC enhanced antibody

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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