Mouse Anti-AQP4 Recombinant Antibody (VS3-CJ1042)
CAT#: VS3-CJ1042
This product is a mouse antibody that recognizes human, mouse, and rat AQP4.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/14784/if_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to cell junctions.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5079/if_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Glial cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
Neuropil
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/14784/141530_B_7_5.jpg
Normal Tissue
Figure 4 Lung
Alveolar cells type I
Staining: High
Intensity: Strong
Quantity: 75%-25%
Alveolar cells type II
Staining: High
Intensity: Strong
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/14784/141530_A_3_4.jpg
Normal Tissue
Figure 5 Kidney
Distal tubules
Staining: Medium
Intensity: Strong
Quantity: <25%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/14784/141530_A_8_5.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced (AF22, RPTEC TERT1)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000171885-AQP4
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Specifications
- Immunogen
- KLH-conjugated synthetic peptide encompassing a sequence of human Aquaporin 4
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human, Mouse, Rat AQP4
- Species Reactivity
- Human, Mouse, Rat
- Applications
- WB, ICC, IF, IHC
- Conjugate
- Unconjugated
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Buffer
- 30% Glycerol, 0.87% NaCl, 0.42% Potassium phosphate, pH7.3.
- Preservative
- 0.01% Sodium Azide
- Storage
- Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipped at 4°C
Applications
- Application Notes
- This antibody has been tested for use in Western Blot (1:1000-1:3000), Immunocytochemistry/Immunofluorescence (1:100-1:200), Immunohistochemistry (1:50-1:200).
Target
- Alternative Names
- MIWC; WCH4; Haqp4
- Gene ID
- 361
- UniProt ID
- P55087
- Sequence Similarities
- Belongs to the MIP/aquaporin (TC 1.A.8) family.
- Cellular Localization
- Cell membrane, Cell projection, Endosome, Membrane
- Post Translation Modifications
- Phosphorylation by PKC at Ser-180 reduces conductance by 50%. Phosphorylation by PKG at Ser-111 in response to glutamate increases conductance by 40% (By similarity).
Isoform 2: Palmitoylated on its N-terminal region. Isoform 1: Not palmitoylated.
- Protein Refseq
- NP_001641.1; NP_004019.1
- Function
- Forms a water-specific channel (PubMed:7559426, PubMed:8601457, PubMed:19383790).
Plays an important role in brain water homeostasis and in glymphatic solute transport. Required for a normal rate of water exchange across the blood brain interface. Required for normal levels of cerebrospinal fluid influx into the brain cortex and parenchyma along paravascular spaces that surround penetrating arteries, and for normal drainage of interstitial fluid along paravenous drainage pathways. Thereby, it is required for normal clearance of solutes from the brain interstitial fluid, including soluble beta-amyloid peptides derived from APP. Plays a redundant role in urinary water homeostasis and urinary concentrating ability (By similarity).
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "AQP4"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1110z | Mouse Anti-AQP4 Recombinant Antibody (clone 28H5) | WB, IP, IF, IHC, FC | Mouse IgG3 |
| MOB-0672MZ | Mouse Anti-AQP4 Recombinant Antibody (clone 4E3) | ELISA, IHC-Fr, IHC-P, IP, WB | Mouse IgG1 |
| VS3-FY92 | Recombinant Mouse Anti-AQP4 Antibody (VS3-FY92) | WB, IHC, IF | Mouse IgG |
| VS4-WK34 | Mouse Anti-AQP4 Recombinant Antibody (VS4-WK34) | ELISA, WB, IHC, IF | Mouse IgG |
| VS3-XY3264 | Rabbit Anti-AQP4 Recombinant Antibody (clone R05-8C3) | IHC-P | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0977CLV | Anti-Human AQP4 Recombinant Antibody (D15107) | ELISA, FuncS, WB, IF, FC | Chimeric antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0223 | Hi-Affi™ Rabbit Anti-AQP4 Recombinant Antibody (clone DS223AB) | IHC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC282 | Recombinant Anti-AQP4 Vesicular Antibody, EV Displayed (VS-0425-YC282) | ELISA, FC, Cell-uptake |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY479 | Anti-AQP4 Immunohistochemistry Kit | IHC | |
| VS-0525-XY480 | Anti-Mouse AQP4 Immunohistochemistry Kit | IHC | |
| VS-0525-XY481 | Anti-Rat AQP4 Immunohistochemistry Kit | IHC |
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