CytoStream™ Rabbit Anti-Aqp5 Polyclonal Antibody (VS13-YC61)
CAT#: VS13-YC61
The CytoStream™ Rabbit Anti-Aqp5 Polyclonal Antibody is specific for human, mouse and rat Aqp5. Aqp5, functioning in protein binding and water channel activity, is expressed in several structures and implicated in eye morphogenesis and saliva secretion, with human orthologs linked to palmoplantar keratoderma. The anti-Aqp5 antibody was developed and selected by our CytoStream™ platform and the mAb is recommended for use in flow cytometry staining (1 μg per 1x 10⁶ cells).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line SiHa
Immunofluorescent staining of human cell line SiHa shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/65008/1712_E3_2_cr5804dac1ad659_selected.jpg
Normal Tissue
Figure 2 High expression in salivary gland
Glandular cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/65008/145416_B_1_1.jpg
Normal Tissue
Figure 3 Colon
Glandular cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/65008/145416_A_9_3.jpg
Normal Tissue
Figure 4 Testis
Elongated or late spermatids Staining: High Intensity: Strong Quantity:>75% Leydig cells Staining: Medium Intensity: Strong Quantity: <25% Pachytene spermatocytes Staining: High Intensity: Strong Quantity:>75% Round or early spermatids Staining: High Intensity: Strong Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/65008/145416_A_4_6.jpg
RNA Expression
Figure 5 RNA cell line category: Cell line enriched (OE19)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000161798-AQP5
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Specifications
- Immunogen
- Recombinant mouse AQP5 (aa 201-265) conjugated with KLH.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat Aqp5
- Species Reactivity
- Human, Mouse, Rat, Dog (Predicted), Pig (Predicted), Cow (Predicted), Horse (Predicted), Sheep (Predicted)
- Applications
- WB, ELISA, IHC-P, IHC-Fr, FC, IF
- Conjugate
- NONE
Product Property
- Clonality
- Polyclonal
- Purification
- Affinity Chromatography purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 0.01× TBS (pH 7.4) containing 50% Glycerol and 1% BSA.
- Preservative
- 0.03% Proclin 300
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- Western blot (WB): 1/500-1/2000
ELISA: 1/5000-1/10000
Immunohistochemistry-paraffin (IHC-P): 1/100-1/500
Immunohistochemistry-frozen (IHC-Fr): 1/100-1/500
Flow cytometry (FC): 1 μg per 1x 10⁶ cells
Immunofluorescence (IF): 1/100-1/500
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "AQP5"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0224 | Hi-Affi™ Rabbit Anti-AQP5 Recombinant Antibody (clone DS224AB) | ICC, IHC-P, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-FY91 | Recombinant Rabbit Anti-AQP5 Antibody (clone R03-9E3) | WB, IHC-F, IHC-P, ICC, IF | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY142 | Anti-AQP5 Immunohistochemistry Kit | IHC | |
| VS-0525-XY482 | Anti-Human AQP5 Immunohistochemistry Kit | IHC |
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