Rabbit Anti-AR Recombinant Antibody (VS3-CJ559)
CAT#: VS3-CJ559
This product is a rabbit antibody that recognizes human AR.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line RH-30
Immunofluorescent staining of human cell line RH-30 shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4733/1914_G6_27_cr5c8779fe8d0bf_selected.jpg
Normal Tissue
Figure 2 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Leydig cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1/135_A_6_6.jpg
Normal Tissue
Figure 3 High expression in seminal vesicle.
Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1/135_A_9_6.jpg
RNA Expression
Figure 4 RNA cell line category: Cell line enhanced (AF22, BJ hTERT+, HAP1, LHCN-M2, RH-30, T-47d)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000169083-AR
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Specifications
- Immunogen
- Recombinant protein
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human AR
- Species Reactivity
- Human
- Applications
- WB, ICC, IF, IHC, FC
- Conjugate
- Unconjugated
Product Property
- Purification
- Protein A affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Buffer
- 40% Glycerol, 1% BSA, TBS, pH7.4.
- Preservative
- 0.05% Sodium Azide
- Storage
- Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry.
Target
- Alternative Names
- KD; AIS; AR8; TFM; DHTR; SBMA; HYSP1; NR3C4; SMAX1; HUMARA
- Gene ID
- 367
- UniProt ID
- P10275
- Sequence Similarities
- Belongs to the nuclear hormone receptor family. NR3 subfamily.
- Cellular Localization
- Cytoplasm, Nucleus
- Post Translation Modifications
- Sumoylated on Lys-388 (major) and Lys-521. Ubiquitinated. Deubiquitinated by USP26. 'Lys-6' and 'Lys-27'-linked polyubiquitination by RNF6 modulates AR transcriptional activity and specificity.
Phosphorylated in prostate cancer cells in response to several growth factors including EGF. Phosphorylation is induced by c-Src kinase (CSK). Tyr-535 is one of the major phosphorylation sites and an increase in phosphorylation and Src kinase activity is associated with prostate cancer progression. Phosphorylation by TNK2 enhances the DNA-binding and transcriptional activity and may be responsible for androgen-independent progression of prostate cancer. Phosphorylation at Ser-83 by CDK9 regulates AR promoter selectivity and cell growth. Phosphorylation by PAK6 leads to AR-mediated transcription inhibition.
Palmitoylated by ZDHHC7 and ZDHHC21. Palmitoylation is required for plasma membrane targeting and for rapid intracellular signaling via ERK and AKT kinases and cAMP generation.
- Protein Refseq
- NP_000035.2; NP_001011645.1; NP_001334990.1
- Function
- Steroid hormone receptors are ligand-activated transcription factors that regulate eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues (PubMed:19022849).
Transcription factor activity is modulated by bound coactivator and corepressor proteins like ZBTB7A that recruits NCOR1 and NCOR2 to the androgen response elements/ARE on target genes, negatively regulating androgen receptor signaling and androgen-induced cell proliferation (PubMed:20812024).
Transcription activation is also down-regulated by NR0B2. Activated, but not phosphorylated, by HIPK3 and ZIPK/DAPK3.
Isoform 3:
Lacks the C-terminal ligand-binding domain and may therefore constitutively activate the transcription of a specific set of genes independently of steroid hormones.
Isoform 4:
Lacks the C-terminal ligand-binding domain and may therefore constitutively activate the transcription of a specific set of genes independently of steroid hormones.
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Related Diseases
Prostate Cancer
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "AR"
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0226 | Hi-Affi™ Rabbit Anti-AR Recombinant Antibody (clone DS226AB) | ICC, IHC-P, IP, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| ZG-0307F | Mouse Anti-AR Recombinant Antibody (ZG-0307F) | WB, ELISA | Mouse IgG |
| ZG-0308F | Mouse Anti-AR Recombinant Antibody (ZG-0308F) | WB, IHC, ELISA | Mouse IgG |
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| ZG-0549J | Rabbit Anti-AR Recombinant Antibody (clone 10A5) | ELISA, IHC | Rabbit IgG |
| VS3-XY3265 | Rabbit Anti-AR Recombinant Antibody (clone R07-6E7) | WB, IHC-P, ICC, IF | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY24 | Rat Anti-NHP AR Recombinant Antibody (clone AN1-15) | WB, IHC, IF | Rat IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY143 | Anti-AR Immunohistochemistry Kit | IHC | |
| VS-0525-XY485 | Anti-Mouse AR Immunohistochemistry Kit | IHC | |
| VS-0525-XY484 | Anti-Human AR Immunohistochemistry Kit | IHC | |
| VS-0525-XY486 | Anti-Monkey AR Immunohistochemistry Kit | IHC |
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