Rabbit Anti-ARNT Recombinant Antibody (clone 2F11) (CAT#: ZG-0550J)
This product is a rabbit antibody that recognizes ARNT.
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Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
We require custom production
WB
Figure 1 Rabbit Anti-ARNT Antibody (ZG-0550J) in WB
Western Blot
Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, HepG2 whole cell lysate, K562 whole cell lysate
All lanes: ARNT antibody at 1.83μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 87, 85, 86 KDa
Observed band size: 87 KDa
IHC
Figure 2 Rabbit Anti-ARNT Antibody (ZG-0550J) in IHC
IHC image of ZG-0550J diluted at 1:183 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IP
Figure 3 Rabbit Anti-ARNT Antibody (ZG-0550J) in IP
Immunoprecipitating HIF-1 beta in Hela whole cell lysate
Lane 1: Rabbit control IgG instead of ZG-0550J in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: ZG-0550J (3μg) + Hela whole cell lysate (500μg)
Lane 3: Hela whole cell lysate (20μg)
Specifications
- Immunogen
- A synthesized peptide derived from human ARNT
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human ARNT
- Species Reactivity
- Human
- Clone
- 2F11
- Applications
- ELISA, WB, IHC, IP
- Conjugate
- Uconjugated
- Related Disease
- Cardiovascular
Product Property
- Purification
- Affinity purified
- Format
- Liquid
- Concentration
- See the COA
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The antibody was validated for Enzyme-linked Immunosorbent Assay, Western Blot, Immunohistochemistry, Immunoprecipitation.
WB:1:500-1:5000, IHC:1:50-1:200, IP:1:200-1:1000
Target
- Alternative Names
- HIF1B; TANGO; bHLHe2; HIF1BETA; HIF-1beta; HIF1-beta; HIF-1-beta; aryl hydrocarbon receptor nuclear translocator; class E basic helix-loop-helix protein 2; dioxin receptor, nuclear translocator; hypoxia-inducible factor 1, beta subunit
- Gene ID
- 405
- UniProt ID
- P27540
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IgG Antibodies
IHC Kits & Antibodies
| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-214 | Human Anti-ARNT Recombinant Antibody (clone U39) | IP, IF | Human IgG1 |
| MRO-0735-CN | Rabbit Anti-ARNT Recombinant Antibody (clone CBACN-269) | WB, IHC | Rabbit IgG |
| ZG-0102C | Mouse Anti-ARNT Recombinant Antibody (clone 4C5) | WB, IHC-P, IF | Mouse IgG |
| VS3-CJ838 | Rabbit Anti-ARNT Recombinant Antibody (VS3-CJ838) | WB, IHC | Rabbit IgG |
| VS3-XY94 | Mouse Anti-ARNT Recombinant Antibody (clone 4D9) | WB |
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