Rabbit Anti-ATF2 (pT71) Recombinant Antibody (clone R07-3E5) (CAT#: VS3-WK1993)
This product is a rabbit recombinant antibody that is specific to phosphorylated ATF2. The target of this antibody is phospho-ATF2 (Thr71). The clone R07-3E5 can be used in WB analysis at a dilution of 1/500-1/1000, IF analysis at a dilution of 1/50-1/200 and IP analysis at a dilution of 1/20. The molecular weight of ATF2 (pThr71) observed in WB experiments using this antibody is 70 kDa.
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/22134/if_selected.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/22134/if_selected.jpg
Figure 2 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3769/if_selected.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3769/if_selected.jpg
Figure 3 IHC staining of human prostate
(Immunohistochemical staining of human prostate shows moderate nuclear positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/22134/ihc_selected.jpg
(Immunohistochemical staining of human prostate shows moderate nuclear positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/22134/ihc_selected.jpg
Figure 4 IHC staining of human cerebellum
(Immunohistochemical staining of human cerebellum shows strong nuclear positivity in Purkinje cells and cells in molecular and granular layer.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3769/11607_B_7_8_selected.jpg
(Immunohistochemical staining of human cerebellum shows strong nuclear positivity in Purkinje cells and cells in molecular and granular layer.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3769/11607_B_7_8_selected.jpg
Figure 5 Lymph node
(Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Non-germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3769/11607_A_8_8.jpg
(Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Non-germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3769/11607_A_8_8.jpg
Figure 6 Cerebral cortex
(Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Glial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3769/11607_B_9_5.jpg
(Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Glial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3769/11607_B_9_5.jpg
Figure 7 Colon
(Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3769/11607_A_9_3.jpg
(Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3769/11607_A_9_3.jpg
Figure 8 Liver
(Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: nuclear Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3769/11607_A_9_4.jpg
(Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: nuclear Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3769/11607_A_9_4.jpg
Figure 9 Kidney
(Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3769/11607_A_7_5.jpg
(Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3769/11607_A_7_5.jpg
Figure 10 Testis
(Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Sertoli cells Staining: Medium Intensity: Moderate Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3769/11607_A_4_6.jpg
(Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Sertoli cells Staining: Medium Intensity: Moderate Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3769/11607_A_4_6.jpg
Figure 11 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000115966-ATF2
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000115966-ATF2
Specifications
- Immunogen
- A synthetic phosphopeptide corresponding to residues Thr71 of human ATF2
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Phospho-Thr71 of human and mouse ATF2
- Species Reactivity
- Human, Mouse
- Clone
- R07-3E5
- Applications
- WB, ICC, IF, IP
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IF: 1/50-1/200
IP: 1/20
Target
- Alternative Names
- Activating Transcription Factor 2; Cyclic AMP-Responsive Element-Binding Protein 2; CAMP Response Element-Binding Protein CRE-BP1; CAMP-Dependent Transcription Factor ATF-2; Histone Acetyltransferase ATF2; CREB-2; CREB2; HB16; Activating Transcription Factor 2 Splice Variant ATF2-Var2
- Gene ID
- 1386
- UniProt ID
- P15336
- Long Name
- Activating Transcription Factor 2
Recommended Products
Isotype Control
Secondary Antibody
Related Resources
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Non-Canonical Wnt Signaling Pathway
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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