Rabbit Anti-ATF2 Recombinant Antibody (clone 4F2) (CAT#: ZG-0551J)
This product is a rabbit antibody that recognizes ATF2.
Specific Inquiry
WB
Figure 1 Rabbit Anti-ATF2 Antibody (ZG-0551J) in WB
Western Blot
Positive WB detected in 293 whole cell lysate,A549 whole cell lysate,HepG2 whole cell lysate(treated with Calyculin A or EGF)
All lanes Phospho-ATF2 antibody at 1.015μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 70 KDa
Observed band size: 70 KDa
IF
Figure 2 Rabbit Anti-ATF2 Antibody (ZG-0551J) in IF
Immunofluorescence staining of A549 cells(treated with 100mM EGF for 20min) with ZG-0551J at 1:63,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG (H+L).
Specifications
- Immunogen
- A synthesized peptide derived from human Phospho-ATF2 (T71)
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human ATF2
- Species Reactivity
- Human
- Clone
- 4F2
- Applications
- ELISA, WB, IF
- Conjugate
- Uconjugated
- Related Disease
- Cancer
Product Property
- Purification
- Affinity purified
- Format
- Liquid
- Concentration
- See the COA
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The antibody was validated for Enzyme-linked Immunosorbent Assay, Western Blot, Immunofluorescence.
WB:1:500-1:5000, IF:1:20-1:200
Target
- Alternative Names
- HB16; CREB2; TREB7; CREB-2; CRE-BP1; cyclic AMP-dependent transcription factor ATF-2; activating transcription factor 2 splice variant ATF2-var2; cAMP response element-binding protein CRE-BP1; cAMP responsive element binding protein 2, formerly; cAMP-dependent transcription factor ATF-2; cyclic AMP-responsive element-binding protein 2; histone acetyltransferase ATF2
- Gene ID
- 1386
- UniProt ID
- P15336
Related Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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See other products for "ATF2"
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IgG Antibody Products
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Antibody Magnetic Beads
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0673MZ | Mouse Anti-ATF2 Recombinant Antibody (clone CBLS-122) | ELISA | Mouse IgG2a |
| MRO-2301-CN | Rabbit Anti-ATF2 Recombinant Antibody (clone CBACN-595) | WB, IF, IP | Rabbit IgG |
| ZG-0315F | Mouse Anti-ATF2 Recombinant Antibody (ZG-0315F) | WB, IHC, ELISA | Mouse IgG |
| VS3-XY99 | Mouse Anti-ATF2 Recombinant Antibody (clone 4F12) | ELISA, WB, IHC | Mouse IgG1 |
| VS3-FY102 | Recombinant Rabbit Anti-ATF2 Antibody (clone R09-8F4) | WB, ICC, IF, IP | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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