AbPlus™ Anti-ATF5 Magnetic Beads (VS-0724-YC423)
CAT#: VS-0724-YC423
The AbPlus Anti-ATF5 Magnetic Beads (VS-0724-YC423) is an innovative affinity resin which is bound with anti-ATF5 specific antibody. The beads were designed for small-scale affinity purification and immunoprecipitation (IP) of ATF5 protein under native and denaturing conditions.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/30187/if_selected.jpg
Normal Tissue
Figure 2 IHC staining of human duodenum
Immunohistochemical staining of human duodenum shows moderate nuclear positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/30187/ihc_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Endothelial cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Glial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/30187/62507_B_7_5.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Peripheral nerve/ganglion Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/30187/62507_A_8_3.jpg
Normal Tissue
Figure 5 Liver
Hepatocytes Staining: Low Intensity: Weak Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/30187/62507_A_7_4.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Cells in tubules Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/30187/62507_A_7_5.jpg
Normal Tissue
Figure 7 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/30187/62507_A_6_6.jpg
Normal Tissue
Figure 8 Lymph node
Germinal center cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Nuclear Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/30187/62507_A_9_8.jpg
RNA Expression
Figure 9 RNA cell line category: Cell line enhanced (HDLM-2, Karpas-707, THP-1, U-266/84, U-698)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000169136-ATF5
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Specifications
- Applications
- Immunoprecipitation, Protein Purification
- Matrix
- Magnetic bead
- Bead Ligand
- Anti-ATF5 specific antibody
- Target
- ATF5
- Immunogen
- Recombinant human ATF5 fragment derived from E. coli.
- Target Species
- Human
- Bead Capacity
- 40 mg/mL
- Bead size
- 25 μm
- Format
- Suspension
- Concentration
- 2 mg/mL
- Buffer
- PBS, pH 7.4
- Preservative
- 0.1% Sodium azide
- Storage
- Stored at 4°C, and is stable for up to 2 years. Do not centrifuge, dry or freeze the magnetic beads.
Applications
- Application Notes
- The beads are in suspension and will settle upon storage. Prior to use, mix the vial gently (do not vortex) to ensure delivery of proper bead volume.
Target
- Introduction
- Transcription factor that either stimulates or represses gene transcription through binding of different DNA regulatory elements such as cAMP response element (CRE) (consensus: 5-GTGACGT[AC][AG]-3), ATF5-specific response element (ARE) (consensus: 5-C[CT]TCT[CT]CCTT[AT]-3) but also the amino acid response element (AARE), present in many viral and cellular promoters. Critically involved, often in a cell type-dependent manner, in cell survival, proliferation, and differentiation (PubMed:10373550, PubMed:15358120, PubMed:21212266, PubMed:20654631). Its transcriptional activity is enhanced by CCND3 and slightly inhibited by CDK4 (PubMed:15358120). Important regulator of the cerebral cortex formation, functions in cerebral cortical neuroprogenitor cells to maintain proliferation and to block differentiation into neurons. Must be down-regulated in order for such cells to exit the cycle and differentiate (By similarity). Participates in the pathways by which SHH promotes cerebellar granule neuron progenitor cells proliferation (By similarity). Critical for survival of mature olfactory sensory neurons (OSN), directs expression of OSN-specific genes (By similarity). May be involved in osteogenic differentiation (PubMed:22442021). Promotes cell proliferation and survival by inducing the expression of EGR1 sinergistically with ELK1. Once acetylated by EP300, binds to ARE sequences on target genes promoters, such as BCL2 and EGR1 (PubMed:21791614). Plays an anti-apoptotic role through the transcriptional regulation of BCL2, this function seems to be cell type-dependent (By similarity). Cooperates with NR1I3/CAR in the transcriptional activation of CYP2B6 in liver (PubMed:18332083). In hepatic cells, represses CRE-dependent transcription and inhibits proliferation by blocking at G2/M phase (PubMed:22528486, PubMed:18701499). May act as a negative regulator of IL1B transduction pathway in liver (PubMed:24379400). Upon IL1B stimulus, cooperates with NLK to activate the transactivation activity of C/EBP subfamily members (PubMed:25512613). Besides its function of transcription factor, acts as a cofactor of CEBPB to activate CEBPA and promote adipocyte differentiation (PubMed:24216764). Regulates centrosome dynamics in a cell-cycle- and centriole-age-dependent manner. Forms 9-foci symmetrical ring scaffold around the mother centriole to control centrosome function and the interaction between centrioles and pericentriolar material (PubMed:26213385).
- Alternative Names
- Activating Transcription Factor 5; CAMP-Dependent Transcription Factor ATF-5; Transcription Factor ATFx; ATFX; Cyclic AMP-Dependent Transcription Factor ATF-5; HMFN0395;
- Gene ID
- 22809
- UniProt ID
- Q9Y2D1
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Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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See other products for "ATF5"
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-0132-CN | Rabbit Anti-ATF5 Recombinant Antibody (clone CBACN-049) | WB, IF, IHC, IP | Rabbit IgG |
| ZG-0554J | Rabbit Anti-ATF5 Recombinant Antibody (clone 9D8) | ELISA, WB, IHC | Rabbit IgG |
| VS3-WK1222 | Rabbit Anti-ATF5 Recombinant Antibody (VS3-WK1222) | WB, ICC, IF, IHC, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
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| VS-0525-XY580 | Anti-ATF5 Immunohistochemistry Kit | IHC | |
| VS-0525-XY581 | Anti-Mouse ATF5 Immunohistochemistry Kit | IHC |
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