Rabbit Anti-ATP5F1B Recombinant Antibody (clone 5F10) (CAT#: ZG-0561J)
This product is a rabbit antibody that recognizes ATP5F1B.
Specific Inquiry
WB
Figure 1 Rabbit Anti-ATP5F1B Antibody (ZG-0561J) in WB
Western Blot
Positive WB detected in: 293T whole cell lysate, HT29 whole cell lysate, HepG2 whole cell lysate, Jurkat whole cell lysate, 293 whole cell lysate, Rat Heart tissue, Mouse Heart tissue
All lanes: ATP5F1B antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 57 kDa
Observed band size: 57 kDa
IHC
Figure 2 Rabbit Anti-ATP5F1B Antibody (ZG-0561J) in IHC
IHC image of ZG-0561J diluted at 1:100 and staining in paraffin-embedded human heart tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
IHC
Figure 3 Rabbit Anti-ATP5F1B Antibody (ZG-0561J) in IHC
IHC image of ZG-0561J diluted at 1:100 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Specifications
- Immunogen
- A synthesized peptide derived from human ATPB
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human ATP5F1B
- Species Reactivity
- Human, Mouse, Rat
- Clone
- 5F10
- Applications
- ELISA, WB, IHC
- Conjugate
- Uconjugated
- Related Disease
- Cancer
Product Property
- Purification
- Affinity purified
- Format
- Liquid
- Concentration
- See the COA
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The antibody was validated for Enzyme-linked Immunosorbent Assay, Western Blot, Immunohistochemistry.
WB:1:500-1:5000, IHC:1:50-1:200
Target
- Alternative Names
- ATP5B; ATPMB; ATPSB; HEL-S-271; ATP synthase subunit beta, mitochondrial; ATP synthase, H+ transporting, mitochondrial F1 complex, beta polypeptide; epididymis secretory protein Li 271; mitochondrial ATP synthase beta subunit; mitochondrial ATP synthetase, beta subunit
- Gene ID
- 506
- UniProt ID
- P06576
Related Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "ATP5F1B"
Select a product category from the dropdown menu below to view related products.
Please select product type
IgG Antibody Products
ScFv Antibody Products
Fab Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-J0040-YC | Mouse Anti-ATP5F1B Recombinant Antibody (clone m7A8) | ELISA, WB, IF, IHC | Mouse IgG |
| HPAB-J0041-YC | Human Anti-ATP5F1B Recombinant Antibody (clone c7A8) | ELISA, WB, IF, IHC | Chimeric (mouse/human) IgG1 |
| VS3-FY119 | Recombinant Rabbit Anti-ATP5F1B Antibody (clone R01-4G1) | WB, IHC-F, IHC-P, ICC, IF, IP | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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