Rabbit Anti-ATP5F1B Recombinant Antibody (clone 5F10) (CAT#: ZG-0561J)
This product is a rabbit antibody that recognizes ATP5F1B.
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WB
Figure 1 Rabbit Anti-ATP5F1B Antibody (ZG-0561J) in WB
Western Blot
Positive WB detected in: 293T whole cell lysate, HT29 whole cell lysate, HepG2 whole cell lysate, Jurkat whole cell lysate, 293 whole cell lysate, Rat Heart tissue, Mouse Heart tissue
All lanes: ATP5F1B antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 57 kDa
Observed band size: 57 kDa
IHC
Figure 2 Rabbit Anti-ATP5F1B Antibody (ZG-0561J) in IHC
IHC image of ZG-0561J diluted at 1:100 and staining in paraffin-embedded human heart tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
IHC
Figure 3 Rabbit Anti-ATP5F1B Antibody (ZG-0561J) in IHC
IHC image of ZG-0561J diluted at 1:100 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Specifications
- Immunogen
- A synthesized peptide derived from human ATPB
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human ATP5F1B
- Species Reactivity
- Human, Mouse, Rat
- Clone
- 5F10
- Applications
- ELISA, WB, IHC
- Conjugate
- Uconjugated
- Related Disease
- Cancer
Product Property
- Purification
- Affinity purified
- Format
- Liquid
- Concentration
- See the COA
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The antibody was validated for Enzyme-linked Immunosorbent Assay, Western Blot, Immunohistochemistry.
WB:1:500-1:5000, IHC:1:50-1:200
Target
- Alternative Names
- ATP5B; ATPMB; ATPSB; HEL-S-271; ATP synthase subunit beta, mitochondrial; ATP synthase, H+ transporting, mitochondrial F1 complex, beta polypeptide; epididymis secretory protein Li 271; mitochondrial ATP synthase beta subunit; mitochondrial ATP synthetase, beta subunit
- Gene ID
- 506
- UniProt ID
- P06576
Related Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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See other products for "ATP5F1B"
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IgG Antibody Products
ScFv Antibody Products
Fab Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-J0040-YC | Mouse Anti-ATP5F1B Recombinant Antibody (clone m7A8) | ELISA, WB, IF, IHC | Mouse IgG |
| HPAB-J0041-YC | Human Anti-ATP5F1B Recombinant Antibody (clone c7A8) | ELISA, WB, IF, IHC | Chimeric (mouse/human) IgG1 |
| VS3-FY119 | Recombinant Rabbit Anti-ATP5F1B Antibody (clone R01-4G1) | WB, IHC-F, IHC-P, ICC, IF, IP | Rabbit IgG |
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