Mouse Anti-AURKA Recombinant Antibody (ZG-0311F)
CAT#: ZG-0311F
This product is a mouse antibody that recognizes AURKA.
Tested Data
Gene Expression
WB
Figure 1 Mouse Anti-AURKA Recombinant Antibody (ZG-0311F) in WB.
Use ARK-1 monoclonal antibodies against HEK293 (1), Sw620 (2), MCF-7 (3), Jurkat (4), HeLa (5), HepG2 (6), Cos7 (7) and PC-12 Western blot analysis (8) Cell lysate.
IHC
Figure 2 Mouse Anti-AURKA Recombinant Antibody (ZG-0311F) in IHC.
Immunohistochemical analysis of paraffin-embedded ovarian cancer (left) and lung cancer (right) by DAB staining with ARK-1 monoclonal antibody.
ELISA
Figure 3 Mouse Anti-AURKA Recombinant Antibody (ZG-0311F) in ELISA.
ELISA analysis of ZG-0311F was performed by coating with human AURKA Protein.
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Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm, cytosol & centrosome.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2636/80_F6_2_selected.jpg
RNA Expression
Figure 2 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000087586-AURKA
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Specifications
- Immunogen
- Recombinant fragment of human ARK-1 expressed in E. coli.
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human AURKA
- Species Reactivity
- Human, Rat, Monkey
- Applications
- Western Blot, Immunohistochemistry, Enzyme-linked Immunosorbent Assay
- Conjugate
- NONE
Product Property
- Format
- Liquid
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C for short term. For long term storage, store at-20°C, avoiding freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/2000
Immunohistochemistry: 1/200-1/1000
ELISA: 1/10000
Target
- Alternative Names
- Aurora Kinase A; Protein Phosphatase 1, Regulatory Subunit 47; Serine/Threonine-Protein Kinase Aurora-A; Serine/Threonine-Protein Kinase 6; Breast Tumor-Amplified Kinase; Aurora/IPL1-Related Kinase 1; Aurora 2; STK15; STK6; ARK1; AURA; BTAK; AIK; Serine/Threonine Protein Kinase 15; Serine/Threonine-Protein Kinase 15; AURKA; AIK; AIRK1; ARK1; AURA; AYK1; BTAK; IAK1; STK15; STK6; Aurora kinase A; Aurora 2; Aurora/IPL1-related kinase 1; ARK-1; Aurora-related kinase 1; hARK1; Breast tumor-amplified kinase; Serine/threonine-protein kinase 15; Serine/threonin
- Gene ID
- 6790
- UniProt ID
- O14965
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "AURKA"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0304 | Hi-Affi™ Rabbit Anti-AURKA Recombinant Antibody (clone DS304AB) | IHC-P, IP, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-0145-CN | Rabbit Anti-AURKA Recombinant Antibody (clone CBACN-055) | WB, IF, FC | Rabbit IgG |
| ZG-0318F | Mouse Anti-AURKA Recombinant Antibody (ZG-0318F) | WB, ICC | Mouse IgG |
| ZG-0562J | Rabbit Anti-AURKA Recombinant Antibody (clone 1H8) | ELISA, IF | Rabbit IgG |
| VS3-QX91 | Mouse Anti-AURKA Recombinant Antibody (clone A4-8D) | WB, ICC, IHC, FC | Mouse IgG2b |
| VS3-CJ132 | Rabbit Anti-AURKA Recombinant Antibody (VS3-CJ132) | WB, ICC, IF, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY42 | Mouse Anti-NHP AURKA Recombinant Antibody (clone 1F8) | WB, FC, ELISA | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY214 | Anti-AURKA Immunohistochemistry Kit | IHC | |
| VS-0525-XY659 | Anti-Mouse AURKA Immunohistochemistry Kit | IHC | |
| VS-0525-XY658 | Anti-Human AURKA Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC81 | CytoStream™ Rabbit Anti-AURKA Recombinant Antibody (VS13-YC81) | WB, ICC, IF, IHC-P, FC | Rabbit IgG |
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