Mouse Anti-B2M Recombinant Antibody (clone 5G12-E9-E1) (CAT#: VS3-QX95)
This product is a mouse antibody that recognizes B2M. The antibody can be used in applications: WB, IHC, ICC, FC, ELISA.
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane, cytosol & the Golgi apparatus.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/6361/if_selected.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane, cytosol & the Golgi apparatus.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/6361/if_selected.jpg
Figure 2 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane, cytosol & the Golgi apparatus.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2572/if_selected.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane, cytosol & the Golgi apparatus.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2572/if_selected.jpg
Figure 3 Spleen
(High expression in spleen)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/6361/18752_B_7_4_val_selected.jpg
(High expression in spleen)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/6361/18752_B_7_4_val_selected.jpg
Figure 4 Bone marrow
(Hematopoietic cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2572/6948_B_6_4.jpg
(Hematopoietic cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2572/6948_B_6_4.jpg
Figure 5 Lymph node
(Non-germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2572/6948_A_8_8.jpg
(Non-germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2572/6948_A_8_8.jpg
Figure 6 Cerebral cortex
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2572/6948_B_8_5.jpg
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2572/6948_B_8_5.jpg
Figure 7 Kidney
(Cells in glomeruli Staining: High Intensity: Strong Quantity: 75%-25% Collecting ducts Staining: Low Intensity: Moderate Quantity: <25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2572/6948_A_8_5.jpg
(Cells in glomeruli Staining: High Intensity: Strong Quantity: 75%-25% Collecting ducts Staining: Low Intensity: Moderate Quantity: <25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2572/6948_A_8_5.jpg
Figure 8 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000166710-B2M
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000166710-B2M
Specifications
- Immunogen
- Purified recombinant fragment of human B2M (aa 21-100) expressed in E. coli.
- Host Species
- Mouse
- Derivation
- Mouse
- Type
- Mouse IgG2a
- Specificity
- β2-microglobulin
- Species Reactivity
- Human
- Clone
- 5G12-E9-E1
- Applications
- WB, IHC, ICC, FC, ELISA
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Store at 4°C for short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1:500-1:2000; IHC: 1:200-1:1000; FC: 1:200-1:400; ELISA: 1:10000
Target
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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IHC Kit and Antibody Products: Precision Tools for Immunohistochemistry
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1431z-F(E) | Recombinant Anti-Human B2M Antibody Fab Fragment | Neut, WB, FuncS | Fab |
| HPAB-0130-FY-F(E) | Human Anti-B2M Recombinant Antibody (clone B2M-1D8); Fab Fragment | ELISA | Humanized Fab |
| HPAB-0131-FY-F(E) | Human Anti-B2M Recombinant Antibody (clone B2M-2F6); Fab Fragment | ELISA | Humanized Fab |
| HPAB-0350-FY-F(E) | Mouse Anti-B2M Recombinant Antibody (clone FY-007); Fab Fragment | ELISA | Mouse Fab |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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