Human Anti-B2M Recombinant Antibody (clone B2M-2F6)
CAT#: HPAB-0131-FY
Recombinant monoclonal antibody to B2M. This product is a recombinant humanized antibody that recognizes human B2M.
Published Data
Gene Expression
ELISA
Figure 1 ELISA result, proves that selected B2M antibodies specific ground suppresses combining of B2M antibody (B2) and B2M.
FC
Figure 2 Flow cytometer result, confirms that selected B2M antibody combines with the B2M specificity of tumor cell surface.
FC
Figure 3 The flow cytometer result, proves that selected B2M antibody can be at external evoked apoptosis of tumor cells.
Fig. A shows the tumour cell of apoptosis for Annexin V dyeing.Fig. B is the result that TUNEL measures.
FC
Figure 4 The flow cytometer result, proves that selected B2M antibody can be at external evoked former generation cloak cell lymphoma cell apoptosis.
Figure 5 Selected B2M antibody does not cause apoptosis external to normal blood cell (comprising PMBC, CD3+T cell, CD16+NK cell and CD19+B cell).
FC
Figure 6 Flow cytometer result has shown that humanization B2M antibody can suppress mouse source B2M antibody and combine with the B2M of tumor cell surface, proves that the humanization B2M antibody that is obtained can combine with the B2M specificity.
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Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane, cytosol & the Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/6361/if_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane, cytosol & the Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2572/if_selected.jpg
Normal Tissue
Figure 3 Spleen
High expression in spleen
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/6361/18752_B_7_4_val_selected.jpg
Normal Tissue
Figure 4 Bone marrow
Hematopoietic cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2572/6948_B_6_4.jpg
Normal Tissue
Figure 5 Lymph node
Non-germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2572/6948_A_8_8.jpg
Normal Tissue
Figure 6 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2572/6948_B_8_5.jpg
Normal Tissue
Figure 7 Kidney
Cells in glomeruli Staining: High Intensity: Strong Quantity: 75%-25% Collecting ducts Staining: Low Intensity: Moderate Quantity: <25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2572/6948_A_8_5.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000166710-B2M
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Specifications
- Immunogen
- Mouse spleen cells with SP2 / 0 myeloma cells by three B2M immunized BALB under action of PEG2000, B2M using antigen-coated indirect ELISA screening anti-B2M antibody positive secreting hybridomas.
- Host Species
- Human
- Derivation
- Humanized
- Type
- Humanized IgG1, λ
- Specificity
- Human B2M
- Species Reactivity
- Human
- Clone
- B2M-2F6
- Applications
- ELISA, FC
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay and Flow Cytometry.
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
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- CAT
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