Recombinant Human Anti-Bacillus anthracis PA Antibody (8A7) (CAT#: HPAB-0633LY)

Figure 1 Protective efficacy of PA-specific human monoclonal antibodies (hMAbs) in vitro and in vivo. Serial 2-fold dilutions of the indicated hMAbs mixed with LeTx were incubated with J774A.1

Serial 2-fold dilutions of the indicated hMAbs mixed with LeTx were incubated with J774A.1 cells for 4 h. The viability of the J774A.1 cells was determined using the MTT assay. Data are means ± SDs from at least three independent experiments, each conducted in duplicate.

Chi, X., Li, J., Liu, W., Wang, X., Yin, K., Liu, J., ... & Zhang, X. (2015). Generation and characterization of human monoclonal antibodies targeting anthrax protective antigen following vaccination with a recombinant protective antigen vaccine. Clinical and Vaccine Immunology, 22(5), 553-560.

Figure 2 Clone 8H6 neutralized PA by inhibiting furin cleavage.

PA was incubated with furin in the presence or absence of each hMAb. Furin cleavage was determined by the absence of the full-length PA83 band and the presence of the PA63 and PA20 bands. NS Ab, nonspecific antibody.

Chi, X., Li, J., Liu, W., Wang, X., Yin, K., Liu, J., ... & Zhang, X. (2015). Generation and characterization of human monoclonal antibodies targeting anthrax protective antigen following vaccination with a recombinant protective antigen vaccine. Clinical and Vaccine Immunology, 22(5), 553-560.