Rat Anti-BAX Recombinant Antibody (clone 3C10) (CAT#: PABC-255)
This product is a recombinant Rat antibody that can recognize BAX. 3C10 inhibits cytosolic BAX by limiting exposure of the membrane-associating helix α9. The 3C10 antibody engages the N-terminal residues (33-41) of the α1-α2 loop.
Specific Inquiry
IP
Figure 1 Immunoprecipitation
3C10 binds non-activated BaxS184L. Membrane fractions from Bak−/−Bax−/− MEFs expressing BaxS184L were immunoprecipitated with the 3C10 or anti-FLAG (negative control) antibodies and immunoblotted for Bax.
Iyer, S., Anwari, K., Alsop, A. E., Yuen, W. S., Huang, D. C., Carroll, J., ... & Kluck, R. M. (2016). Identification of an activation site in Bak and mitochondrial Bax triggered by antibodies. Nature communications, 7, 11734.
ELISA
Figure 2 Epitope mapping
The 3C10 epitope maps to the Bax α1–α2 loop, close to α1. Immunoreactivity of the rat monoclonal antibody, clone 3C10, towards biotinylated human Bax peptides as determined by enzyme-linked immunosorbent assay. X axis indicates Bax residue number or control reactions. Mean and s.d. of three independent experiments.
Iyer, S., Anwari, K., Alsop, A. E., Yuen, W. S., Huang, D. C., Carroll, J., ... & Kluck, R. M. (2016). Identification of an activation site in Bak and mitochondrial Bax triggered by antibodies. Nature communications, 7, 11734.
WB
Figure 3 3C10 binds WT Bax.
Lysates of Bak−/−Bax−/− MEFs expressing Bax (Exp.) or of Bak−/−Bax−/− membranes reconstituted with recombinant Bax (100 nM, Rec.) were immunoprecipitated with 3C10 and immunoblotted for Bax.
Iyer, S., Anwari, K., Alsop, A. E., Yuen, W. S., Huang, D. C., Carroll, J., ... & Kluck, R. M. (2016). Identification of an activation site in Bak and mitochondrial Bax triggered by antibodies. Nature communications, 7, 11734.
Specifications
- Host Species
- Rat
- Type
- Rat IgG
- Specificity
- Human BAX
- Species Reactivity
- Human
- Clone
- 3C10
- Applications
- IP, ELISA, WB, Activ, Inhib
Product Property
- Purity
- >95% as determined by analysis by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The antibody 3C10 has been reported in applications of Immunoprecipitation, Enzyme-linked Immunosorbent Assay, Western Blot, Activation, Inhibition. It's recommended that the optimal antibody concentration, dilution, incubition time etc. be carefully titrated in specific assays.
Activation: The concentration 0.1 mg/mL of this antibody can be used for activation.
Target
- Alternative Names
- BCL2-Associated X Protein; Bcl-2-Like Protein 4; Bcl2-L-4; BCL2L4; BCL2-Associated X Protein Omega; Baxdelta2G9omega; Baxdelta2omega; Baxdelta2G9
- Gene ID
- 581
- UniProt ID
- Q07812
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Non-Human Primate (NHP) Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-101-F(E) | Recombinant Anti-human BAX Antibody Fab Fragment | IP, FuncS | Fab |
| PFBC-255 | Rat Anti-BAX Recombinant Antibody (clone 3C10); Fab Fragment | ELISA, WB, Inhib | Rat Fab |
| HPAB-2961LY-F(E) | Human Anti-BAX Recombinant Antibody; Fab Fragment (HPAB-2961LY-F(E)) | ELISA, Inhib | Human Fab |
| HPAB-2962LY-F(E) | Human Anti-BAX Recombinant Antibody; Fab Fragment (HPAB-2962LY-F(E)) | ELISA, Inhib | Human Fab |
| HPAB-2963LY-F(E) | Human Anti-BAX Recombinant Antibody; Fab Fragment (HPAB-2963LY-F(E)) | ELISA, Inhib | Human Fab |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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