Mouse Anti-BAX Recombinant Antibody (clone 6F11)

Name: Mouse Anti-BAX Recombinant Antibody (clone 6F11)
SKU: ZG-0045J
Rating: 5 (1 reviews)

CAT#: ZG-0045J

This product is a mouse antibody that recognizes BAX.

Tested Data Gene Expression

Figure 1 Mouse Anti-BAX Recombinant Antibody (clone 6F11) in WB

Western blot analysis of lysates from 1) Hela Cell Lysate, 2) C2C12 Cell Lysate, 3) PC12 Cell Lysate cells, (Green) primary Antibody was diluted at 1:1000, 4°C over night, Dylight 800 secondary Antibody was diluted at 1:10000, 37°C 1 hour. (Red) Actin β Polyclonal Antibody Antibody was diluted at 1:5000 as loading control, 4°Cover night, Dylight 680 secondary Antibody was diluted at 1:10000, 37°C1 hour.

IHC

Figure 2 Mouse Anti-BAX Recombinant Antibody (clone 6F11) in IHC

Immunohistochemical analysis of paraffin-embedded Human-Tonsil tissue. 1.Bax Mouse Monoclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for Antibody retrieval(>98°C, 20min). 3, Secondary Antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary Antibody only.

IHC

Figure 3 Mouse Anti-BAX Recombinant Antibody (clone 6F11) in IHC

Immunohistochemical analysis of paraffin-embedded Rat-spleen tissue. 1.Bax Mouse Monoclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for Antibody retrieval(>98°C, 20min). 3, Secondary Antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary Antibody only.

Figure 4 Mouse Anti-BAX Recombinant Antibody (clone 6F11) in WB

Western blot analysis of 1) Hela Cell Lysate, 2) C2C12 Cell Lysate, 3) PC12 Cell Lysate using Bax Mouse mAb diluted at 1:1000.

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Normal Tissue Figure 1 Cerebral cortex

Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Glial cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Neuronal cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4206/13374_B_7_5.jpg

Normal Tissue Figure 2 Colon

Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4206/13374_A_7_3.jpg

Normal Tissue Figure 3 Liver

Cholangiocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4206/13374_A_9_4.jpg

Normal Tissue Figure 4 Kidney

Cells in glomeruli
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Cells in tubules
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4206/13374_A_7_5.jpg

Normal Tissue Figure 5 Testis

Cells in seminiferous ducts
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Leydig cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4206/13374_A_4_6.jpg

Normal Tissue Figure 6 Lymph node

Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4206/13374_A_7_8.jpg

RNA Expression Figure 7 RNA cell line category: Low cell line specificity

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000087088-BAX

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Specifications

Immunogen
Synthetic Peptide of Bax at AA range of 30-110

Host Species
Mouse

Type
Mouse IgG

Specificity
Human BAX

Species Reactivity
Human, Rat, Mouse, Chicken

Clone
6F11

Applications
IF, ICC, WB, IHC-P

Product Property

Storage
Store at 4°C for short term. For long term storage, store at-20°C, avoiding freeze/thaw cycles.

Applications

Application Notes
The antibody was validated for Immunofluorescence/Immunocytochemistry, Western Blot, Immunohistochemistry-Paraffin.

Target

Alternative Names
BAX; BCL2-associated X protein; BAX; BCL2-associated X protein; BCL2L

Gene ID
581

UniProt ID
Q07812

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Related Diseases

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Related Signaling Pathways

p53 Signaling Pathway

Downloadable Resources

Download resources about recombinant antibody development and antibody engineering to boost your research.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - ZG-0045J.pdf

MSDS

MSDS - ZG-0045J.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

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Protocol & Troubleshooting

Immunofluorescence (IF)

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

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