AbPlus™ Anti-BCAM Magnetic Beads (BRIC 221)
CAT#: VS-0424-XY28
The AbPlus Anti-BCAM Magnetic Beads (BRIC 221) is an innovative affinity resin which is bound with anti-BCAM specific antibody (BRIC 221). The beads were designed for small-scale affinity purification and immunoprecipitation (IP) of BCAM protein under native and denaturing conditions.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoli fibrillar center.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5654/if_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoli fibrillar center.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5654/607_G4_1_blue_red_green.jpg
Subcellular Location
Figure 3 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoli fibrillar center.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5654/604_G4_1_blue_red_green.jpg
Normal Tissue
Figure 4 Cerebral cortex
Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5654/55689_B_9_5.jpg
Normal Tissue
Figure 5 Colon
Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5654/55689_A_9_3.jpg
Normal Tissue
Figure 6 Kidney
Bowman's capsule
Staining: High
Intensity: Strong
Quantity:>75%
Collecting ducts
Staining: High
Intensity: Strong
Quantity:>75%
Distal tubules
Staining: High
Intensity: Strong
Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5654/55689_A_8_5.jpg
Normal Tissue
Figure 7 Testis
Leydig cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5654/55689_A_6_6.jpg
RNA Expression
Figure 8 RNA cell line category: Cell line enhanced (BEWO, HaCaT, SK-BR-3, T-47d, U-2 OS)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000187244-BCAM
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Specifications
- Applications
- Immunoprecipitation, Protein Purification
- Matrix
- Magrose bead (> 50 μmol/mL gel)
- Bead Ligand
- Anti-BCAM specific antibody (BRIC 221)
- Target
- BCAM
- Immunogen
- Erythrocytes
- Target Species
- Human
- Antibody Clone
- BRIC 221
- Bead Capacity
- 20-30 mg/mL binding antibody
- Bead size
- 10-37 μm
- Stability
- pH 2-14
- Format
- 20% Suspension
- Buffer
- PBS, pH 7.4, with 1% BSA
- Preservative
- 0.03% Proclin 300
- Storage
- Stored at 4°C, and is stable for up to 2 years. Do not centrifuge, dry or freeze the magnetic beads.
Applications
- Application Notes
- The beads are in suspension and will settle upon storage. Prior to use, mix the vial gently (do not vortex) to ensure delivery of proper bead volume.
Target
- Introduction
- This gene encodes Lutheran blood group glycoprotein, a member of the immunoglobulin superfamily and a receptor for the extracellular matrix protein, laminin. The protein contains five extracellular immunoglobulin domains, a single transmembrane domain, and a short C-terminal cytoplasmic tail. This protein may play a role in epithelial cell cancer and in vaso-occlusion of red blood cells in sickle cell disease. Polymorphisms in this gene define some of the antigens in the Lutheran system and also the Auberger system. Inactivating variants of this gene result in the recessive Lutheran null phenotype, Lu(a-b-), of the Lutheran blood group. Two transcript variants encoding different isoforms have been found for this gene.
- Alternative Names
- AU; CD239; LU; MSK19
- Gene ID
- 4059
- UniProt ID
- P50895
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Downloadable Resources
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Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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