CytoStream™ Rabbit Anti-BCAM Recombinant Antibody (VS13-YC91) (CAT#: VS13-YC91)
The CytoStream™ Rabbit Anti-BCAM Recombinant Antibody is specific for human, mouse and rat BCAM. BCAM encodes the Lutheran blood group glycoprotein, part of the immunoglobulin superfamily, serving as a receptor for laminin and potentially involved in epithelial cell cancer and sickle cell disease-related vaso-occlusion. It includes polymorphisms defining Lutheran and Auberger blood group antigens, with two known transcript variants. The anti-BCAM antibody was developed and selected by our CytoStream™ platform and the mAb is recommended for use in flow cytometry staining (1/50-1/100).
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoli fibrillar center.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5654/if_selected.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoli fibrillar center.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5654/if_selected.jpg
Figure 2 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoli fibrillar center.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5654/607_G4_1_blue_red_green.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoli fibrillar center.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5654/607_G4_1_blue_red_green.jpg
Figure 3 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoli fibrillar center.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5654/604_G4_1_blue_red_green.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoli fibrillar center.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5654/604_G4_1_blue_red_green.jpg
Figure 4 Cerebral cortex
(Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5654/55689_B_9_5.jpg
(Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5654/55689_B_9_5.jpg
Figure 5 Colon
(Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5654/55689_A_9_3.jpg
(Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5654/55689_A_9_3.jpg
Figure 6 Kidney
(Bowman's capsule
Staining: High
Intensity: Strong
Quantity:>75%
Collecting ducts
Staining: High
Intensity: Strong
Quantity:>75%
Distal tubules
Staining: High
Intensity: Strong
Quantity: 75%-25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5654/55689_A_8_5.jpg
(Bowman's capsule
Staining: High
Intensity: Strong
Quantity:>75%
Collecting ducts
Staining: High
Intensity: Strong
Quantity:>75%
Distal tubules
Staining: High
Intensity: Strong
Quantity: 75%-25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5654/55689_A_8_5.jpg
Figure 7 Testis
(Leydig cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5654/55689_A_6_6.jpg
(Leydig cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5654/55689_A_6_6.jpg
Figure 8 RNA cell line category: Cell line enhanced (BEWO, HaCaT, SK-BR-3, T-47d, U-2 OS)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000187244-BCAM
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000187244-BCAM
Figure 8 RNA cell line category: Cell line enhanced (BEWO, HaCaT, SK-BR-3, T-47d, U-2 OS)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000187244-BCAM
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000187244-BCAM
Specifications
- Immunogen
- Recombinant human CD239.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat BCAM
- Species Reactivity
- Human, Mouse, Rat
- Applications
- WB, IHC-P, IP, FC
- Conjugate
- NONE
- Related Disease
- Sickle cell disease
Product Property
- Clonality
- Monoclonal
- Purification
- Affinity Chromatography purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS (pH 7.4) containing 150 mM NaCl and 50% Glycerol.
- Preservative
- 0.02% Sodium azide
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- Western blot (WB): 1/500-1/1000
Immunohistochemistry (IHC): 1/50-1/100
Immunoprecipitation (IP): 1/50
Flow cytometry (FC): 1/50-1/100
Target
- Alternative Names
- Basal Cell Adhesion Molecule (Lutheran Blood Group); Basal Cell Adhesion Molecule (Lu And Au Blood Groups); B-CAM Cell Surface Glycoprotein; Auberger B Antigen; F8/G253 Antigen; MSK19; LU; Lutheran Blood Group (Auberger B Antigen Included); Lutheran Blood Group Variant LUGA;
- Gene ID
- 4059
- UniProt ID
- P50895
- Long Name
- Basal Cell Adhesion Molecule (Lutheran Blood Group)
Recommended Products
Secondary Antibody
Isotype Control
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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ScFv-Fc Chimera Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1207z | Mouse Anti-BCAM Recombinant Antibody (clone 38G1) | WB, IP, IF, ELISA | Mouse IgG2b, κ |
| FAMAB-0449WJ | Human Anti-BCAM Recombinant Antibody (clone A1) | ELISA, WB, FC | Human IgG |
| FAMAB-0450WJ | Human Anti-BCAM Recombinant Antibody (clone A4) | ELISA, WB, FC | Human IgG |
| FAMAB-0451WJ | Human Anti-BCAM Recombinant Antibody (clone B8) | ELISA, WB, FC | Human IgG |
| FAMAB-0452WJ | Human Anti-BCAM Recombinant Antibody (clone B11) | ELISA, WB, FC | Human IgG |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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