Mouse Anti-BCAP31 Recombinant Antibody (VS3-FY2631)
CAT#: VS3-FY2631
The anti-BCAP31 product is a recombinant mouse monoclonal antibody that binds to BAP31 protein, a calculated weight of 30.0 kDa. It shows capability of cross-reacting with human and mouse. The monoclonal antibody can be used in the area of Deafness, Dystonia, and Cerebral Hypomyelination and Adrenoleukodystrophy. This monoclonal antibody can be used in Western Blot, Immunohistochemistry.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to endoplasmic reticulum.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3906/if_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to endoplasmic reticulum.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/15350/if_selected.jpg
Normal Tissue
Figure 3 IHC staining of human pancreas
Immunohistochemical staining of human pancreas shows cytoplasmic positivity in exocrine glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/15350/ihc_selected.jpg
Normal Tissue
Figure 4 Testis
Cells in seminiferous ducts Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3906/11734_A_5_6.jpg
Normal Tissue
Figure 5 Lymph node
Germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3906/11734_A_9_8.jpg
Normal Tissue
Figure 6 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3906/11734_B_8_5.jpg
Normal Tissue
Figure 7 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3906/11734_A_7_3.jpg
Normal Tissue
Figure 8 Liver
Cholangiocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3906/11734_A_8_4.jpg
Normal Tissue
Figure 9 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3906/11734_A_9_5.jpg
RNA Expression
Figure 10 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000185825-BCAP31
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Specifications
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- B cell receptor associated protein 31 of human and mouse origins
- Species Reactivity
- Human, Mouse
- Applications
- WB, IHC
- Conjugate
- Unconjugated, FITC
- MW
- The molecular weight of the target protein is approximately 30.0 kDa.
- Related Disease
- Deafness, Dystonia, And Cerebral Hypomyelination and Adrenoleukodystrophy
Product Property
- Clonality
- Monoclonal Antibody
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/2000
Immunohistochemistry: 1/50-1/200
Target
- Alternative Names
- B Cell Receptor Associated Protein 31; C6-AG Tumor-Associated Antigen; DXS1357E; BAP31; B-Cell Receptor-Associated Protein 31; B-Cell Receptor Associated Protein 31; BCR-Associated Protein Bap31; BCR-Associated Protein 31
- Gene ID
- 10134
- UniProt ID
- P51572
- Sequence Similarities
- Belongs to the BCAP29/BCAP31 family.
- Cellular Localization
- Endoplasmic reticulum membrane; Multi-pass membrane protein
Endoplasmic reticulum-Golgi intermediate compartment membrane; Multi-pass membrane protein
- Post Translation Modifications
- Cleaved by CASP8 and other caspases.
Ubiquitination at Lys79, Lys95, Lys149, Lys158, Lys159, Lys167, Lys185, and Lys204
Modification sites at PhosphoSitePlus
Glycosylation from GlyGen 1 site, 1 O-linked glycan (1 site)
- Protein Refseq
- NP_001132913.1; NP_001132929.1; NP_001243376.1; NP_005736.3
- Function
- Functions as a chaperone protein (PubMed:9396746, 18287538).
Is one of the most abundant endoplasmic reticulum (ER) proteins (PubMed:9396746, 18287538).
Plays a role in the export of secreted proteins in the ER, the recognition of abnormally folded protein and their targeting to the ER associated-degradation (ERAD) (PubMed:9396746, 18287538).
Also serves as a cargo receptor for the export of transmembrane proteins (By similarity).
Plays a role in the assembly of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) by stimulating the translocation of NDUFS4 and NDUFB11 from the cytosol to the mitochondria via interaction with TOMM40.
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "BCAP31"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2978z | Mouse Anti-BCAP31 Recombinant Antibody (clone 35H7) | WB, ELISA | Mouse IgG1, κ |
| MOB-0676MZ | Mouse Anti-BCAP31 Recombinant Antibody (clone 8B4CC7) | FC, IP, WB | Mouse IgG1 |
| MRO-0154-CN | Rabbit Anti-BCAP31 Recombinant Antibody (clone JG37-81) | WB, IF, IHC, FC | Rabbit IgG |
| VS3-CJ1018 | Mouse Anti-BCAP31 Recombinant Antibody (VS3-CJ1018) | WB, IHC | Mouse IgG1, κ |
| VS4-WK46 | Rabbit Anti-BCAP31 Recombinant Antibody (clone 12G12) | ELISA, WB, IHC, IF, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0336 | Hi-Affi™ Rabbit Anti-BCAP31 Recombinant Antibody (clone DS336AB) | FC, IF, IHC-P, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-CN0401 | PE-A*02:01/Human BCAP31 (KLDVGNAEV) MHC Tetramer | FCM, IHC | |
| MHC-CN0402 | APC-A*02:01/Human BCAP31 (KLDVGNAEV) MHC Tetramer | FCM, IHC | |
| MHC-CN0403 | FITC-A*02:01/Human BCAP31 (KLDVGNAEV) MHC Tetramer | FCM, IHC | |
| MHC-CN0404 | A*02:01/Human BCAP31 (KLDVGNAEV) MHC Tetramer | FCM, IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY226 | Anti-BCAP31 Immunohistochemistry Kit | IHC | |
| VS-0525-XY710 | Anti-Human BCAP31 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC92 | CytoStream™ Rabbit Anti-BCAP31 Recombinant Antibody (VS13-YC92) | WB, IF, ICC, IHC-P, FC | Rabbit IgG |
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