Mouse Anti-BCAP31 Recombinant Antibody (VS3-FY2631) (CAT#: VS3-FY2631)
The anti-BCAP31 product is a recombinant mouse monoclonal antibody that binds to BAP31 protein, a calculated weight of 30.0 kDa. It shows capability of cross-reacting with human and mouse. The monoclonal antibody can be used in the area of Deafness, Dystonia, and Cerebral Hypomyelination and Adrenoleukodystrophy. This monoclonal antibody can be used in Western Blot, Immunohistochemistry.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to endoplasmic reticulum.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3906/if_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to endoplasmic reticulum.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3906/if_selected.jpg
Figure 2 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to endoplasmic reticulum.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/15350/if_selected.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to endoplasmic reticulum.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/15350/if_selected.jpg
Figure 3 IHC staining of human pancreas
(Immunohistochemical staining of human pancreas shows cytoplasmic positivity in exocrine glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/15350/ihc_selected.jpg
(Immunohistochemical staining of human pancreas shows cytoplasmic positivity in exocrine glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/15350/ihc_selected.jpg
Figure 4 Testis
(Cells in seminiferous ducts Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3906/11734_A_5_6.jpg
(Cells in seminiferous ducts Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3906/11734_A_5_6.jpg
Figure 5 Lymph node
(Germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3906/11734_A_9_8.jpg
(Germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3906/11734_A_9_8.jpg
Figure 6 Cerebral cortex
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3906/11734_B_8_5.jpg
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3906/11734_B_8_5.jpg
Figure 7 Colon
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3906/11734_A_7_3.jpg
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3906/11734_A_7_3.jpg
Figure 8 Liver
(Cholangiocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3906/11734_A_8_4.jpg
(Cholangiocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3906/11734_A_8_4.jpg
Figure 9 Kidney
(Cells in glomeruli Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3906/11734_A_9_5.jpg
(Cells in glomeruli Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3906/11734_A_9_5.jpg
Figure 10 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000185825-BCAP31
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000185825-BCAP31
Specifications
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- B cell receptor associated protein 31 of human and mouse origins
- Species Reactivity
- Human, Mouse
- Applications
- WB, IHC
- Conjugate
- Unconjugated, FITC
- MW
- The molecular weight of the target protein is approximately 30.0 kDa.
- Related Disease
- Deafness, Dystonia, And Cerebral Hypomyelination and Adrenoleukodystrophy
Product Property
- Clonality
- Monoclonal Antibody
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/2000
Immunohistochemistry: 1/50-1/200
Target
- Alternative Names
- B Cell Receptor Associated Protein 31; C6-AG Tumor-Associated Antigen; DXS1357E; BAP31; B-Cell Receptor-Associated Protein 31; B-Cell Receptor Associated Protein 31; BCR-Associated Protein Bap31; BCR-Associated Protein 31
- Gene ID
- 10134
- UniProt ID
- P51572
- Sequence Similarities
- Belongs to the BCAP29/BCAP31 family.
- Cellular Localization
- Endoplasmic reticulum membrane; Multi-pass membrane protein
Endoplasmic reticulum-Golgi intermediate compartment membrane; Multi-pass membrane protein
- Post Translation Modifications
- Cleaved by CASP8 and other caspases.
Ubiquitination at Lys79, Lys95, Lys149, Lys158, Lys159, Lys167, Lys185, and Lys204
Modification sites at PhosphoSitePlus
Glycosylation from GlyGen 1 site, 1 O-linked glycan (1 site)
- Protein Refseq
- NP_001132913.1; NP_001132929.1; NP_001243376.1; NP_005736.3
- Function
- Functions as a chaperone protein (PubMed:9396746, 18287538).
Is one of the most abundant endoplasmic reticulum (ER) proteins (PubMed:9396746, 18287538).
Plays a role in the export of secreted proteins in the ER, the recognition of abnormally folded protein and their targeting to the ER associated-degradation (ERAD) (PubMed:9396746, 18287538).
Also serves as a cargo receptor for the export of transmembrane proteins (By similarity).
Plays a role in the assembly of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) by stimulating the translocation of NDUFS4 and NDUFB11 from the cytosol to the mitochondria via interaction with TOMM40.
Recommended Products
Secondary Antibody
Isotype Control
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2978z | Mouse Anti-BCAP31 Recombinant Antibody (clone 35H7) | WB, ELISA | Mouse IgG1, κ |
| MOB-0676MZ | Mouse Anti-BCAP31 Recombinant Antibody (clone 8B4CC7) | FC, IP, WB | Mouse IgG1 |
| MRO-0154-CN | Rabbit Anti-BCAP31 Recombinant Antibody (clone JG37-81) | WB, IF, IHC, FC | Rabbit IgG |
| VS3-CJ1018 | Mouse Anti-BCAP31 Recombinant Antibody (VS3-CJ1018) | WB, IHC | Mouse IgG1, κ |
| VS4-WK46 | Rabbit Anti-BCAP31 Recombinant Antibody (clone 12G12) | ELISA, WB, IHC, IF, FC | Rabbit IgG |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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