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Rabbit Anti-BCL2 Recombinant Antibody (clone P21-P), FITC

Rabbit Anti-BCL2 Recombinant Antibody (clone P21-P), FITC (CAT#: ZG-0421U)

Name: Rabbit Anti-BCL2 Recombinant Antibody (clone P21-P), FITC
SKU: ZG-0421U
Rating: 5 (1 reviews)

Rabbit anti-BCL2 antibody (clone P21-P) was conjugated with FITC under optimal conditions. The FITC-conjugated antibody product displayed high specificity against the BCL2 protein of Human. And the solution is free of unconjugated FITC and unconjugated antibody.

Tested Data
Gene Expression
Datasheet
MSDS
COA

Figure 1 Rabbit Anti-BCL2 Antibody (ZG-0421U) in FC

Figure 1 Rabbit Anti-BCL2 Antibody (ZG-0421U) in FC

Profile of Bcl2 expression in human Hek293 cell line analyzed by the BD FACSCanto II. HEK293 cells were fixed, permeabilized and stained with anti-Bcl2 FITC (blue, 10 μl per test) or with an isotype control (black).

Subcellular Location

Figure 1 IF staining of human cell line SK-MEL-30
(Immunofluorescent staining of human cell line SK-MEL-30 shows localization to nucleoplasm & nuclear membrane.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/55295/1484_G3_6_selected.jpg

Normal Tissue

Figure 2 Thyroid gland
(Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3/155167_B_3_5.jpg

Figure 3 Colon
(Mucosal lymphoid cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Peripheral nerve/ganglion
Staining: High
Intensity: Strong
Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3/155167_A_7_3.jpg

Figure 4 Kidney
(Cells in tubules
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3/155167_A_8_5.jpg

Figure 5 Lymph node
(Germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3/155167_A_7_8.jpg

RNA Expression

Figure 6 RNA cell line category: Cell line enhanced (HDLM-2, HL-60, Karpas-707, U-266/70)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000171791-BCL2

Datasheet

Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number

Specifications

Host Species
Rabbit

Type
Rabbit IgG

Specificity
Human BCL2

Species Reactivity
Human

Clone
P21-P

Applications
FC

Conjugate
FITC

Related Disease
Follicular Lymphoma 1 and High-Grade B-Cell Lymphoma Double-Hit/Triple-Hit.

Product Property

Buffer
Aqueous buffer solution containing 10mg/ml of BSA and 0.05% sodium azide.

Preservative
0.05% sodium azide

Storage
Store at 4°C, in the dark. Do not freeze.

Applications

Application Notes
This antibody has been used 10 µL per test for Flow Cytometry. Specific conditions should be optimized by the end user.

Target

Alternative Names
Bcl-2; PPP1R50; apoptosis regulator Bcl-2; B-cell CLL/lymphoma 2; protein phosphatase 1, regulatory subunit 50

Gene ID
596

UniProt ID
P10415

Related Resources

Related Signaling Pathways
Related Diseases

Hedgehog Signaling Pathway

p53 Signaling Pathway

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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