CytoStream™ Rabbit Anti-BCL2L1 Recombinant Antibody (VS13-YC97)
CAT#: VS13-YC97
The CytoStream™ Rabbit Anti-BCL2L1 Recombinant Antibody is specific for human BCL2L1. BCL2L1 encodes a BCL-2 family protein, regulating mitochondrial membrane potentials and reactive oxygen species, with longer isoforms inhibiting apoptosis and shorter ones activating it, resulting from alternative splicing. The anti-BCL2L1 antibody was developed and selected by our CytoStream™ platform and the mAb is recommended for use in flow cytometry staining (1/50-1/100).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line MCF7
Immunofluorescent staining of human cell line MCF7 shows localization to mitochondria.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/72801/1349_F9_5_selected.jpg
Normal Tissue
Figure 2 IHC staining of human urinary bladder
Immunohistochemical staining of human urinary bladder shows moderate cytoplasmic positivity in urothelial cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/35734/ihc_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuropil Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/35734/143255_B_8_5.jpg
Normal Tissue
Figure 4 Liver
Hepatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/35734/143255_A_9_4.jpg
Normal Tissue
Figure 5 Kidney
Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/35734/143255_A_8_5.jpg
Normal Tissue
Figure 6 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/35734/143255_A_6_6.jpg
Normal Tissue
Figure 7 Lymph node
Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/35734/143255_A_8_8.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000171552-BCL2L1
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Specifications
- Immunogen
- Recombinant human Bcl-XL, aa 37-86.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human BCL2L1
- Species Reactivity
- Human
- Applications
- WB, ICC, IF, IHC-P, FC, IP
- Conjugate
- NONE
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- TBS (pH 7.4) containing 40% Glycerol and 0.05% BSA.
- Preservative
- 0.05% Sodium azide
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- Western blot (WB): 1/1000-1/5000
Immunocytochemistry (ICC): 1/50-1/200
Immunofluorescence (IF): 1/50-1/200
Immunohistochemistry-paraffin (IHC-P): 1/50-1/1000
Flow cytometry (FC): 1/50-1/100
Target
- Alternative Names
- BCL2 Like 1; Protein Phosphatase 1, Regulatory Subunit 52; Apoptosis Regulator Bcl-X; BCL2L; BCLX; Bcl-2-Like Protein 1;
- Gene ID
- 598
- UniProt ID
- Q07817
- Long Name
- BCL2 Like 1
- Cellular Localization
- Nucleus; Mitochondrion inner membrane; Mitochondrion matrix; Mitochondrion outer membrane; Cytoplasmic vesicle; Cytoplasm membrane
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Related Diseases
Amyotrophic Lateral Sclerosis
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "BCL2L1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1570z | Mouse Anti-BCL2L1 Recombinant Antibody (clone 24B10) | ELISA, IF, FuncS | Mouse IgG2a |
| MOB-250YJL | Rabbit Anti-BCL2L1 Recombinant Antibody (clone CBB235) | ELISA | Rabbit IgG |
| ZG-0710F | Mouse Anti-BCL2L1 Recombinant Antibody (clone 2H12) | WB | Mouse IgG2a |
| VS3-FY153 | Recombinant Rabbit Anti-BCL2L1 Antibody (clone R02-2D3) | WB, IHC-F, IHC-P, ICC, IF, IP | Rabbit IgG |
| VS-0723-WK214 | Rabbit Anti-BCL2L1 Recombinant Antibody (VS-0723-WK214) | ELISA, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0068MZ | Chicken Anti-BCL2L1 Polyclonal IgY | WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0344 | Hi-Affi™ Rabbit Anti-BCL2L1 Recombinant Antibody (clone DS344AB) | WB, FC, IP, IHC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-CN0425 | PE-A*02:01/Human BCL2L1 (YLNDHLEPWI) MHC Tetramer | FCM, IHC | |
| MHC-CN0426 | APC-A*02:01/Human BCL2L1 (YLNDHLEPWI) MHC Tetramer | FCM, IHC | |
| MHC-CN0427 | FITC-A*02:01/Human BCL2L1 (YLNDHLEPWI) MHC Tetramer | FCM, IHC | |
| MHC-YF328 | A*03:01/Human BCL-2L1 (RIAAWMATY) MHC Pentamer | FCM | |
| MHC-YF329 | A*02:01/Human BCL-X (YLNDHLEPWI) MHC Pentamer | FCM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC1434 | AbPlus™ Anti-BCL2L1 Magnetic Beads (VS-0724-YC1434) | IP, Protein Purification | |
| VS-0724-YC1435 | AbPlus™ Anti-Bcl2l1 Magnetic Beads (VS-0724-YC1435) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY232 | Anti-BCL2L1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY733 | Anti-Mouse BCL2L1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY732 | Anti-Human BCL2L1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY734 | Anti-Porcine BCL2L1 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC20 | Recombinant Anti-BCL2L1 Vesicular Antibody, EV Displayed (VS-0425-YC20) | ELISA, FC, Cell-uptake |
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