Mouse Anti-BECN1 Recombinant Antibody (ZG-0329F)
CAT#: ZG-0329F
This product is a mouse antibody that recognizes BECN1.
Tested Data
Gene Expression
WB
Figure 1 Mouse Anti-BECN1 Recombinant Antibody (ZG-0329F) in WB.
Western blot detection of Beclin 1 in Hela, 3T3, C6, COS7, CHO-K1, rat brain and Jurkat cell lysates was performed using Beclin 1 mouse mAb (1:1000 dilution). Predicted band size: 52KDa. Observed band size: 55KDa.
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Subcellular Location
Figure 1 IF staining of human cell line SiHa
Immunofluorescent staining of human cell line SiHa shows localization to nuclear bodies & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3949/1891_D2_8_cr600ff296b7030_selected.jpg
Normal Tissue
Figure 2 IHC staining of human cerebellum
Immunohistochemical staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/28949/ihc_selected.jpg
Normal Tissue
Figure 3 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10143/25093_A_9_8.jpg
Normal Tissue
Figure 4 Cerebral cortex
Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10143/25093_B_7_5.jpg
Normal Tissue
Figure 5 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10143/25093_A_7_3.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10143/25093_A_7_5.jpg
Normal Tissue
Figure 7 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10143/25093_A_6_6.jpg
Normal Tissue
Figure 8 Placenta
Trophoblastic cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10143/25093_A_2_7.jpg
RNA Expression
Figure 9 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000126581-BECN1
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Specifications
- Immunogen
- Recombinant human Beclin 1 protein fragments expressed in E. coli.
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human BECN1
- Species Reactivity
- Human, Mouse, Rat, Monkey, Hamster
- Applications
- Western Blot, Immunoprecipitation
- Conjugate
- NONE
Product Property
- Format
- Liquid
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C for short term. For long term storage, store at-20°C, avoiding freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1:1000
Immunoprecipitation: 1:50-200
Target
- Alternative Names
- Beclin 1; Coiled-Coil Myosin-Like BCL2-Interacting Protein; Beclin 1, Autophagy Related; Beclin 1 (Coiled-Coil, Moesin-Like BCL2 Interacting Protein); Beclin 1 (Coiled-Coil, Moesin-Like BCL2-Interacting Protein); ATG6 Autophagy Related 6 Homolog (S. Cerevisiae); Testis Secretory Sperm-Binding Protein Li 215e; ATG6 Autophagy Related 6 Homolog; APG6; ATG 6; ATG6; ATG6 autophagy related 6 homolog; Bcl-2-interacting protein beclin; Beclin 1 (coiled coil moesin like BCL2 interacting protein); Beclin 1 autophagy related; Beclin-1; Beclin1; BECN 1; BECN1; BECN1_HUMAN; Coiled coil myosin like BCL2 interacting protein; Coiled-coil myosin-like BCL2-interacting protein; GT197; Protein GT197; VPS 30; VPS30
- Gene ID
- 8678
- UniProt ID
- Q14457
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "BECN1"
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0352 | Hi-Affi™ Rabbit Anti-BECN1 Recombinant Antibody (clone DS352AB) | IF, IHC-P, IP, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-QX116 | Mouse Anti-BECN1 Recombinant Antibody (clone 5H8-7A4-5D7) | IHC | Mouse IgG1 |
| VS3-QX117 | Mouse Anti-BECN1 Recombinant Antibody (clone 10H3-2F4-7H2) | IHC | Mouse IgG1 |
| VS3-QX118 | Mouse Anti-BECN1 Recombinant Antibody (clone 1C1-9F10-8C2) | WB, IHC | Mouse IgG1 |
| VS3-QX119 | Mouse Anti-BECN1 Recombinant Antibody (clone 2A4) | WB, IHC, ICC, FC, ELISA | Mouse IgG1 |
| VS7-HM225 | Mouse Anti-BECN1 Recombinant Antibody (clone CBL069HM) | WB, IHC-p, IF, FCM, ELISA | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY30 | AbPlus™ Anti-BECN1 Magnetic Beads (VS-0424-XY30) | IP, Protein Purification | |
| VS-0724-YC1274 | AbPlus™ Anti-BECN1 Magnetic Beads (VS-0724-YC1274) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY238 | Anti-BECN1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY756 | Anti-Mouse BECN1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY755 | Anti-Human BECN1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY757 | Anti-Rat BECN1 Immunohistochemistry Kit | IHC |
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