CytoStream™ Rabbit Anti-BRD2 Recombinant Antibody (VS13-YC107)
CAT#: VS13-YC107
The CytoStream™ Rabbit Anti-BRD2 Recombinant Antibody is specific for human BRD2. BRD2, a transcriptional regulator in the BET family, associates with transcription complexes and chromatin, possibly linked to juvenile myoclonic epilepsy, with several splice variants. The anti-BRD2 antibody was developed and selected by our CytoStream™ platform and the mAb is recommended for use in flow cytometry staining (1/50).
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IHC staining of human testis
Immunohistochemical staining of human testis shows strong nuclear positivity in spermatogonia.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/42816/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/42816/486_H3_1_red_green.jpg
Subcellular Location and Protein Expression
Figure 3 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear speckles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/42816/490_H3_1_red_green.jpg
Subcellular Location and Protein Expression
Figure 4 IF staining of human cell line U-251MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nuclear speckles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/42816/509_H3_1_red_green.jpg
Normal Tissue
Figure 5 Colon
Glandular cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/42816/92904_A_7_3.jpg
Normal Tissue
Figure 6 Testis
Pachytene spermatocytes
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Preleptotene spermatocytes
Staining:High
Intensity: Strong
Quantity:>75%
Round or early spermatids
Staining:High
Intensity: Strong
Quantity:>75%
Spermatogonia cells
Staining:High
Intensity: Strong
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/42816/92904_A_4_6.jpg
Normal Tissue
Figure 7 Lymph node
Germinal center cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/42816/92904_A_7_8.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000204256-BRD2
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Specifications
- Immunogen
- Recombinant human BRD2, aa 1-50.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human BRD2
- Species Reactivity
- Human
- Applications
- WB, ICC, IF, IHC-P, FC
- Conjugate
- NONE
- Related Disease
- Epilepsy, Myoclonic Juvenile and Nut Midline Carcinoma
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- TBS (pH 7.4) containing 40% Glycerol and 0.05% BSA.
- Preservative
- 0.05% Sodium azide
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- Western blot (WB): 1/500
Immunocytochemistry (ICC): 1/50
Immunohistochemistry-paraffin (IHC-P): 1/100-1/400
Flow cytometry (FC): 1/50
Target
- Alternative Names
- Bromodomain Containing 2; Really Interesting New Gene 3 Protein; BRD2 Intronic Transcript 1; O27.1.1; RING3; Female Sterile Homeotic-Related Gene 1; Bromodomain-Containing Protein 2; Bromodomain-Containing 2;
- Gene ID
- 6046
- UniProt ID
- P25440
- Long Name
- Bromodomain Containing 2
- Sequence Similarities
- Belongs to the BET family.
- Cellular Localization
- Nucleus
- Protein Refseq
- XP_047275178.1; XP_047275179.1; XP_054185819.1; XP_054185820.1; XP_054186593.1
- Function
- Chromatin reader protein that specifically recognizes and binds histone H4 acetylated at 'Lys-5' and 'Lys-12' (H4K5ac and H4K12ac, respectively), thereby controlling gene expression and remodeling chromatin structures.
Recruits transcription factors and coactivators to target gene sites, and activates RNA polymerase II machinery for transcriptional elongation.
Plays a key role in genome compartmentalization via its association with CTCF and cohesin: recruited to chromatin by CTCF and promotes formation of topologically associating domains (TADs) via its ability to bind acetylated histones, contributing to CTCF boundary formation and enhancer insulation.
Also recognizes and binds acetylated non-histone proteins, such as STAT3.
Involved in inflammatory response by regulating differentiation of naive CD4⁺ T-cells into T-helper Th17: recognizes and binds STAT3 acetylated at 'Lys-87', promoting STAT3 recruitment to chromatin.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "BRD2"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-246 | Human Anti-BRD2 Recombinant Antibody (clone D380) | ELISA, IP-MS | Human IgG1 |
| MRO-0191-CN | Rabbit Anti-BRD2 Recombinant Antibody (clone JG40-42) | WB, IF, IHC, FC | Rabbit IgG |
| HPAB-0353-FY | Mouse Anti-BRD2 Recombinant Antibody (HPAB-0353-FY) | WB, ELISA, Cyt, FuncS | Mouse IgG1 |
| HPAB-0354-FY | Mouse Anti-BRD2 Recombinant Antibody (HPAB-0354-FY) | ELISA, Cyt | Mouse IgG2a, κ |
| VS7-HM257 | Mouse Anti-BRD2 Recombinant Antibody (clone 7C1B10) | WB, FCM | Mouse IgG2b |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0383 | Hi-Affi™ Rabbit Anti-BRD2 Recombinant Antibody (clone DS383AB) | WB, FC, IF, IHC-P | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0355-FY | Mouse Anti-BRD2 Recombinant Antibody (HPAB-0355-FY) | Neut, ELISA, Cyt, FuncS | Mouse IgM, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0353-FY-S(P) | Mouse Anti-BRD2 Recombinant Antibody; scFv Fragment (HPAB-0353-FY-S(P)) | WB, ELISA | Mouse scFv |
| HPAB-0354-FY-S(P) | Mouse Anti-BRD2 Recombinant Antibody; scFv Fragment (HPAB-0354-FY-S(P)) | ELISA | Mouse scFv |
| HPAB-0355-FY-S(P) | Mouse Anti-BRD2 Recombinant Antibody; scFv Fragment (HPAB-0355-FY-S(P)) | ELISA | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0353-FY-F(E) | Mouse Anti-BRD2 Recombinant Antibody; Fab Fragment (HPAB-0353-FY-F(E)) | WB, ELISA | Mouse Fab |
| HPAB-0354-FY-F(E) | Mouse Anti-BRD2 Recombinant Antibody; Fab Fragment (HPAB-0354-FY-F(E)) | ELISA | Mouse Fab, κ |
| HPAB-0355-FY-F(E) | Mouse Anti-BRD2 Recombinant Antibody; Fab Fragment (HPAB-0355-FY-F(E)) | ELISA | Mouse Fab, κ |
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