Rabbit Anti-BUB1 Recombinant Antibody (clone 9C11)
CAT#: ZG-0574J
This product is a rabbit antibody that recognizes BUB1.
Tested Data
Gene Expression
WB
Figure 1 Rabbit Anti-BUB1 Antibody (ZG-0574J) in WB
Western Blot
Positive WB detected in: Hela whole cell lysate, K562 whole cell lysate, HepG2 whole cell lysate
All lanes: Bub1 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 123, 116, 120 kDa
Observed band size: 130 kDa
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Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm, cytosol & vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/6123/967_C5_4_selected.jpg
RNA Expression
Figure 2 RNA cell line category: Cell line enhanced (HEL)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000169679-BUB1
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Specifications
- Immunogen
- A synthesized peptide derived from human Bub1
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human BUB1
- Species Reactivity
- Human
- Clone
- 9C11
- Applications
- ELISA, WB
- Conjugate
- Uconjugated
- Related Disease
- Cancer
Product Property
- Purification
- Affinity purified
- Format
- Liquid
- Concentration
- See the COA
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The antibody was validated for Enzyme-linked Immunosorbent Assay, Western Blot.
WB:1:500-1:5000
Target
- Alternative Names
- BUB1A; BUB1L; hBUB1; mitotic checkpoint serine/threonine-protein kinase BUB1; BUB1 budding uninhibited by benzimidazoles 1 homolog; budding uninhibited by benzimidazoles 1 homolog; mitotic spindle checkpoint kinase; putative serine/threonine-protein kinase
- Gene ID
- 699
- UniProt ID
- O43683
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "BUB1"
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0944MZ | Mouse Anti-BUB1 Recombinant Antibody (clone CBLS-179) | IF | Mouse IgG1 |
| VS1-YJ16 | Mouse Anti-BUB1 Recombinant Antibody (clone 14H5) | IF, WB, IP | Mouse IgG1 |
| VS3-XY3281 | Rabbit Anti-BUB1 Recombinant Antibody (clone R05-1E2) | WB, IHC-P | Rabbit IgG |
| VS7-HM274 | Mouse Anti-BUB1 Recombinant Antibody (clone 1A11C7) | FCM | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0395 | Hi-Affi™ Rabbit Anti-BUB1 Recombinant Antibody (clone DS395AB) | WB, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY860 | Anti-BUB1 Immunohistochemistry Kit | IHC |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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